ダナフォーム List of Products and Services

Starting from an affordable 40,000 yen, we offer a variety of analysis plans. This plan includes data analysis, USB delivery, and operations on a web portal, making it an astonishingly comprehensive data package. We provide plans that cover everything from read counts to strand information. By utilizing three types of library preparation kits, we can accommodate a wide range of species, from mammals like humans and mice to plants, fish, yeast, and bacteria such as lactic acid bacteria. Common concerns include: - How many reads should I obtain? - Should I focus on full length or the 3' end? - Do I really need strand information? If you're unsure about analysis conditions or plans, please feel free to consult with us.

• Analysis and sequencing services

Collaboration plan between Qiagen and our company, Danaform. Contract services for four types of panels targeting bacteria, viruses, and antibiotic resistance genes. Each panel is priced at 80,000 yen per sample, with quantity discounts available starting from 50,000 yen.

• Analysis and sequencing services

Introducing specific factors associated with batch effects in RNA-seq.

• Other bio-related products and services

CAGE-seq and RNA-seq each have their own advantages and differences.

• Analysis and sequencing services

When outsourcing analysis, there may be concerns about the quality of RNA depending on the sample. Please read this as a hint for such situations.

• Analysis and sequencing services

When outsourcing analysis, concerns about the number of samples and sample size are common. Please read this as a hint for such situations.

• Analysis and sequencing services

One of the unique features of the RNA expression analysis method "CAGE-seq" is the "detection of transcription start sites (TSS)." There are instances where multiple TSS are found, or new TSS that are not registered in databases are discovered. We will introduce such cases as reading material.

• Analysis and sequencing services

Although it is not a product, we would like to introduce inquiries that are occasionally received as reading material.

• Analysis and sequencing services

In the conventional "CAGE" analysis, total RNA is input, allowing for the following: - RNA expression analysis (serving the same role as general RNA-seq) - Identification of transcription start sites (the greatest strength of CAGE, which is insufficient with RNA-seq) - Searching for transcription factor binding motifs based on the identified transcription start site data (unique to CAGE, a reverse search function of ChIP-seq) The central principle of library preparation is the "cap trap method," a unique technology developed by RIKEN that captures the cap structure at the 5' end of RNA. In this "NET-CAGE" analysis, "NET-RNA," which is currently being synthesized in the nucleus, is extracted and recovered using a unique technique and input for library preparation. (NET-RNA: Negative Elongating-Transcript: newly synthesized RNA) Generally, the lifespan of total RNA is relatively long, ranging from several hours to several days. In contrast, the eRNA (enhancer RNA) from NET-CAGE has an extremely short lifespan, being degraded within just a few minutes. "CAGE" allows us to observe the total RNA present in the cell, while "NET-CAGE" enables us to see the NET-RNA that is currently being synthesized. It has become possible to differentiate between the points we want to observe.

• Other Nucleic Acid/DNA Research
• Other bio-related products and services
• Other cell research

RNA-seq is one of the NGS sequencing contract analysis methods that comprehensively analyzes gene expression (transcriptome). It can accommodate various species and analyze the expression levels of genes and transcripts as well as splicing patterns. ■ Comprehensive analysis of the transcriptome ■ Analysis of expression levels of genes and transcripts and splicing patterns ■ Identification of novel transcripts ■ Proven results in various species, including model organisms. (e.g., humans, mice, plants, fish, frogs, yeast, bacteria, etc.) Our company also offers a unique technology contract analysis service called CAGE-seq. ■ PCR-free, providing excellent quantitativeness, with detection sensitivity approximately 7 times higher than RNA-seq analysis ■ Strong in detecting RNA transcription start sites ■ Applicable to eukaryotic organisms with genomic information, including model organisms ■ There are examples of usage where the differentiation of transcription start sites in cancer and diseases has been utilized for biomarker candidate exploration. * We are currently offering a booklet that clearly introduces the principles and benefits. It explains the differences between RNA-seq and CAGE-seq, as well as their technologies and prices in an easy-to-understand FAQ format.

• Analysis and sequencing services

Our NGS contract services come with data analysis included, ensuring peace of mind even for first-time users. For expression analysis, simply send Total RNA for "RNA-seq" or "CAGE," and we will deliver the analysis data via USB or HDD. We also offer rRNA removal and de novo assembly tailored to your needs. "CAGE" allows for more quantitative gene expression analysis compared to "RNA-seq." "NET-CAGE" is a new technology for enhancer RNA analysis. Other services include "ATAC-seq," "ChIP-seq," "WES," "WGS," and more. The MGI DNBSEQ sequencer has been fully operational since autumn 2021, with an expansion in autumn 2022 and the introduction of the Illumina NextSeq 2000. 【Features】 ■ The submitted RNA for RNA-seq is total RNA 1ug ■ For RNA-seq, starting with 10ng is possible for human and mouse samples ■ Analysis of a single cell is available, and Ultra Low RNA-seq is a popular plan ■ RNA extraction option ■ We also provide unnecessary RNA removal and de novo assembly tailored to your objectives *Please feel free to contact us for arrangements regarding analysis conditions.

• Analysis and sequencing services
• Other analysis and sequence
• Other Analysis

The patient simulator "LEONARDO-kun Essential" responds according to physiological conditions. It simulates situations such as pulse, blinking, coughing, groaning, electrocardiograms, seizures, ventilator malfunction, airway obstruction, and critical conditions, allowing you to experience the implementation of emergency first aid and coping methods.

• Other medical supplies and cosmetic manufacturing materials

We offer a "contract service for RNA-seq analysis." Our service allows for genome-wide expression analysis unique to NGS, enabling comprehensive detection of genes ranging from low to high expression levels. We can also explore novel transcripts and splicing variants. Additionally, we have a plan with the shortest delivery time of "3 weeks." (This applies when the analysis conditions are 50+25bp paired-end or 75bp paired-end.) We can also provide proposals tailored to your needs. 【Features】 ■ Active in various institutions, including companies, research institutions, and universities, regardless of industry or academia ■ Analysis is possible for any organism that possesses RNA, whether eukaryotic or prokaryotic ■ By combining with ChIP-seq, it is possible to confirm the activity of transcription factors from expression levels *We are currently offering materials that clearly introduce the principles and benefits of RNA-seq! For more details, please check the PDF download. Feel free to contact us with any inquiries.

• others

"LapVision" is a laparoscopic surgery simulator that enables the standardization, structuring, and supplementation of manual training. This product provides high-precision sensory feedback that is crucial for training practice through patented haptic feedback. Additionally, the software includes various modules, featuring different types of blood vessels and exercises that increase in difficulty. 【Features】 ■ Can be installed in various training rooms ■ Standardization of training is possible ■ Haptic feedback ■ Exercise modules for basic skills and medical procedures training ■ Abundant educational content *For more details, please refer to the PDF materials or feel free to contact us.

• Other Machine Parts
• others
• Other medical supplies and cosmetic manufacturing materials

"AngioVision" is an endovascular treatment simulator that allows training without causing invasiveness to actual patients, enabling users to make mistakes in a controlled and safe environment. It features a virtual model of the patient's arterial system based on actual patient images and 3D technology. The software of this product offers a wide range of training, tools, and functions. It is designed not only for instructors but also as an effective educational base for behavior training in rules and knowledge to safely perform endovascular procedures. [Features] ■ Can be installed in various training rooms ■ Standardization of training is possible ■ Haptic feedback - Suitable for training in radiology, cardiology, and neurosurgery ■ Capable of operating actual endovascular treatment devices ■ Can handle multiple virtual endovascular treatment devices *For more details, please refer to the PDF materials or feel free to contact us.

• Other Machine Parts
• others

Essential for gene research, gene analysis and genetic testing. Differences in various analysis methods We are currently offering a free booklet in FAQ format titled "Differences in Gene Expression Analysis Methods" that explains the benefits of CAGE and compares it with other analysis methods! The vague question of "What is CAGE?" may transform into a gentle hope of "CAGE might be good!" You will also understand the principles of the analysis and the required amount of RNA sample. 【Examples of FAQ】 ■ What are the advantages compared to microarrays? ■ How compatible is it with ChIP-seq? ■ How should I send Total RNA? *You can view it by clicking the "PDF Download" button below. *Feel free to contact us with any inquiries.

• Analysis and sequencing services

We explain the benefits of CAGE and compare it with other analysis methods in a FAQ format. The vague question of "What is CAGE?" may transform into a gentle hope of "CAGE might be good!" You will also understand the principles of the analysis and the required amount of RNA sample.

• Analysis and sequencing services

　↓　↓　↓　

• Analysis and sequencing services

We offer a contract analysis service using the technology 'CAGE' to quantitatively analyze the expression of mRNA and ncRNA at each transcription start site. It has approximately seven times the detection sensitivity compared to RNA-seq analysis, and because it is PCR-free, it excels in quantitativeness, providing comprehensive expression data. We provide thorough support for the analysis. The delivered data includes standard sets for expression levels, expression comparisons, clustering, and more. Quality checks of sample RNA and data analysis are part of the basic service, with no additional costs. This service is also recommended for first-time users or those concerned about data analysis. 【Features】 ■ Jointly developed technology with RIKEN ■ Genome-wide quantitative analysis of transcription start sites ■ Comprehensive expression analysis not only of genes but also of promoters ■ Enables exploration of gene expression networks at the promoter level ■ Standard implementation of transcription factor binding motif exploration ■ The price is approximately 100,000 yen per sample, including analysis *For more details about the product, please refer to the PDF document below. *You can also download the booklet "Differences in Gene Expression Analysis Methods as Seen in FAQs" for free from the link below.

• Gene expression analysis contract
• Other Nucleic Acid/DNA Research
• Other bio-related products and services

We offer a "qPCR Double-Labeled Probe Design and Synthesis Service." We take care of the tedious design of fluorescent probes and primers. Please utilize this service to save on total research costs and time. Additionally, we also introduce a "Bulk Scale Oligo Synthesis Service" that can synthesize guaranteed yields of 400 nmol in 5 days. 【Features】 ■ For qPCR ■ Saves on total research costs and time ■ If the probe does not function, we will design and resynthesize a second one for free ■ Simply order along with your gene information ■ Choose from two types of dyes and quenchers *For more details, please refer to the PDF document or feel free to contact us.

• Others Synthesis
• DNA primer custom synthesis
• Other contract services

"SINEUP" is a new tool that contributes to the analysis of gene function, which has been conducted so far using Knock-IN/OUT and Knock-Down methods such as RNAi. By increasing translation efficiency, it can enhance the expression of the target protein. Furthermore, it can be used in conjunction with other expression systems. 【Features】 ■ Provides a new direction for gene research ■ Up to 10 times increase in protein expression is expected ■ Functions simply by adding to the expression system ■ Compatible with other expression systems *For more details, please refer to the PDF materials or feel free to contact us.

• Other protein analysis

We introduce examples of significant contributions to FANTOM, which has developed and expanded around the field of transcriptome analysis. At FANTOM, we analyzed the expression profiles of mature miRNAs and primary transcripts in numerous primary cultured cells using short RNA-seq analysis and the CAGE method. Furthermore, the CAGE method revealed these promoter sequences. As a result, we demonstrated that miRNA promoters are highly conserved, there is a strong correlation between the expression profiles of mature miRNAs and primary transcripts, and there is a strong correlation in the expression profiles among multiple miRNAs derived from the same primary transcript. *For more details, please refer to the PDF materials or feel free to contact us.*

• DNA sequencer
• Analysis and sequencing services
• Gene expression analysis contract

"Eprobe PCR Reagent" is an enzyme suitable for the Eprobe PCR method. The Eprobe PCR method is a technology that applies the newly developed fluorescent probe Eprobe, created in collaboration between our company and RIKEN, to gene detection using PCR. It features capabilities such as real-time PCR detection and melt curve analysis using a single probe, allowing for a wide range of applications across various fields. Additionally, we offer custom synthesis of probes (Eprobe/Eprimer) used in the Eprobe PCR method. 【Features】 ■ Co-developed fluorescent probe Eprobe ■ Real-time PCR detection and melt curve analysis can be performed with a single probe ■ Custom synthesis of probes (Eprobe/Eprimer) is available *For more details, please refer to the PDF document or feel free to contact us.

• Other research reagents
• Other restriction enzymes

The "isothermal nucleic acid amplification reagent" is an enzyme reagent suitable for isothermal nucleic acid amplification methods such as the SmartAmp method. We offer a lineup that includes a thermophilic strand-displacing enzyme capable of synthesizing new DNA strands, a reaction buffer for Aac DNA Polymerase, and a reverse transcriptase with stable properties even at high temperatures. 【Lineup】 ■ Thermophilic strand-displacing enzyme / Aac DNA Polymerase ■ Reaction buffer for Aac DNA Polymerase / 2x Reaction Buffer ■ Reverse transcriptase / AMV Reverse Transcriptase *For more details, please refer to the PDF document or feel free to contact us.

• Other research reagents
• Other restriction enzymes

The "SmartAmp Kit" is a product that uses the new gene detection technology "SmartAmp method," which amplifies and detects genes specifically from a single drop of blood in a simple, rapid, and cost-effective manner. It can utilize existing real-time PCR devices as they are, and since it does not require temperature fluctuations like PCR methods, it allows for a reduction in amplification time and energy consumption, making it an environmentally friendly technology. Additionally, the simplification of the device is also possible. 【Features】 ■ Amplification reaction proceeds isothermally at 60°C ■ Using a characteristic primer set and a novel strand-displacing DNA polymerase, the amplification reaction is completed in about 30 minutes ■ The operation involves simply adding pre-treated peripheral blood to the reaction solution ■ Patented domestic technology ■ The enzyme is also developed in-house, ensuring stable supply *For more details, please refer to the PDF document or feel free to contact us.

• Other analytical and testing equipment
• Gene expression analysis contract

Danaform Inc. aims to be a company that satisfies "customers," "shareholders," and "employees" as a brain trust with high research capabilities and quality technology, specializing in the bio business, and contributing to the world as a venture from RIKEN. We sell library preparation kits for the CAGE method as a new solution for gene expression analysis, and we conduct analysis from custom synthesis of libraries. Additionally, we also provide contract manufacturing of cDNA libraries with high full-length rates and low duplication rates. 【Business Activities】 ■ Gene expression analysis using CAGE method and NGS ■ Manufacturing and sales of nucleic acid detection-related products ■ Custom synthesis of fluorescent oligos ■ Distribution of various clones *For more details, please refer to our catalog or feel free to contact us.

• Other bio products
• Other analytical and testing equipment
• Other research reagents

"Eprobe/Eprimer" is quenching in a single-stranded state, but emits fluorescence by specifically hybridizing with the complementary strand. When applied to PCR methods or SmartAmp methods, it is a fluorescent probe that can detect the target nucleic acid sequence with extreme sharpness. SNP analysis can be performed with a single probe in one tube. It has a higher Tm compared to unlabelled oligo DNA, enabling the detection of trace somatic mutations using PCR clamping methods. 【Features】 ■ High S/N ratio ■ Fluorescent detection possible through specific hybridization ■ Real-time PCR detection and melting curve analysis possible with a single probe *For more details, please refer to the catalog or feel free to contact us.

• Other microscopes and optical inspection equipment
• Other bio-related products and services
• Other analytical and testing equipment

ChIP-seq is an analytical method that can identify genomic regions where DNA-binding proteins, such as transcription factors, and histones that have specific modifications are bound. It identifies genome-wide DNA binding sites for transcription factors and other proteins. It explores the mechanisms of gene expression regulation through epigenetics, such as histone methylation. It provides candidate biomarkers for cancer and complex diseases. 【Features】 ■ Identifies genome-wide DNA binding sites for proteins ■ Explores mechanisms of gene expression regulation ■ Provides candidate biomarkers for complex diseases *For more details, please refer to the catalog or feel free to contact us.

• Analysis and sequencing services

RNA-seq is one of the next-generation sequencing analyses, a method for comprehensively detecting gene expression known as the transcriptome. It can accommodate various species and allows for the analysis of gene and transcript expression levels as well as splicing patterns. For model organisms, results can be delivered in as little as 3 to 4 weeks. A data confirmation guide (a booklet) is included with the delivery, making it reassuring for beginners. 【Features】 - We offer cost-effective plans and DNBseq sequencing contract services. - Identification of novel transcripts. - Total RNA starting from 1μg or 10ng is possible. - Proven results with various species, including model organisms (humans, mice, plants, fish, frogs, yeast, bacteria, etc.). *We are currently offering a booklet that clearly introduces the principles and benefits. You can view it immediately via "PDF download."

• Analysis and sequencing services

This catalog is the product catalog of Danaform Co., Ltd., which mainly distributes various clones and conducts gene expression analysis. We offer a wide range of resources for cDNA, genomic, shRNA, and RNAi clones from important model organisms for life science research, including the "RIKEN Human cDNA Clones," which far exceed the human MGC collection with 311,600 full-length human cDNA clones, as well as "Honeybee cDNA Clones" and "Mouse cDNA Clones." We have a rich selection, so please choose the products you need. [Contents] ■ Product Lineup *For more details, please refer to the catalog or feel free to contact us.

• RNA samples
• RNA samples

"CAGE" is a technology that allows for the quantification of gene transcription products at each transcription start site and enables genome-wide quantitative analysis of promoter activity. This allows for the accurate capture of transcriptional promoters and facilitates detailed profiling of expression. It serves as a powerful tool in genome annotation research from a new perspective, such as elucidating the cascade of transcriptional signal transduction. [Features] ■ High sensitivity technology ■ Large-scale and rapid analysis ■ Low cost ■ Excellent quantitativeness ■ Potential to discover new RNA molecules and promoters *For more details, please refer to the catalog or feel free to contact us.

• DNA sequencer