Unchained Labs (アンチェインドラブズ） List of News Articles

The SMPD division of Takeda Pharmaceutical Company has significantly evolved its high-throughput experimentation (HTE) and automation capabilities over the past few years. At the core of this evolution are the tools and devices from Unchained Labs, which offer high compatibility and enable an integrated comprehensive solution. Our ultimate goal is to build Self-Driving Labs (SDLs) that execute self-optimizing workflows utilizing AI and algorithms. Extensive parameter exploration is essential for process development and optimization, and iterative, data-driven SDLs demonstrate their true value, especially when combined with automated HTE synthesis platforms. In this webinar, we will present a vision for the future of fully automated optimization HTE workflows that can accommodate various applications in synthetic molecular pharmaceutical development. Additionally, we will delve into current success stories of existing hardware and software, as well as the challenges we face at this point through real-world examples.

**[Challenge]** Even with high-purity samples like cell culture samples, accurately characterizing extracellular vesicles (EVs) is not easy. Interference from lipoproteins, cellular debris, and protein aggregates poses challenges. Additionally, the complexity of biological fluids, limited sample volumes, and the presence of rare EV subpopulations further complicate the analysis. **[Solution]** Our Leprechaun separates EVs on Luni consumables, allowing for the measurement of particle size and concentration, and simultaneous analysis of up to four surface or internal markers. It can be applied to samples of less than 25µL, does not require sample purification, and offers sensitivity up to 5×10⁵ particles/mL, single particle analysis, and EV size measurement down to 35nm. **[Demonstration]** In this webinar, we will introduce how Leprechaun combines single particle interference measurement with immunofluorescence imaging to achieve comprehensive analysis of any EV sample. It is also possible to confirm the loading efficiency of cargo carried by EVs, distinguish whether proteins are soluble or EV-derived, and obtain a breakdown of all EV subpopulations.

We have launched a new product. Please check the link below for details.

**[Issue]** The evolution of LNPs is progressing more rapidly than ever, largely due to modifications in particle formulations. However, the formulations to be tested are complex in composition and involve a vast number of combinations, making large-scale screening difficult to achieve. Furthermore, there is the subsequent challenge of transferring to production. Evaluations are also required to be accurate, comprehensive, and high-throughput. **[Webinar Overview]** In this webinar, we will explain how our Sun series can accelerate the exploration and development of LNPs, based on the latest research findings and user case studies in LNP formulations. We will also introduce Stunner, which enables high-speed and accurate LNP evaluations.

Our company will be exhibiting at the 25th Annual Meeting of the Protein Science Society, which will be held from June 18 (Wednesday) to June 20 (Friday), 2025. At our booth, we will introduce groundbreaking solutions for the research and development of proteins and antibody drugs, including the Aunty stability evaluation device, which can also be automated. We invite you to visit our booth. Dates: June 18 (Wednesday) - June 20 (Friday), 2025 Venue: Akurie Himeji Details: https://aeplan.jp/pssj2025/

Unchained Labs, Inc. will exhibit at the 41st Annual Meeting of the Japanese DDS Society, which will be held from June 17 (Tuesday). At our booth, you can see a wide range of products, from the manufacturing of lipid nanoparticles (LNP) to their characterization, as well as products for ADC and the evaluation of extracellular vesicles. Additionally, on the first day of the event, July 17, we will also hold a presentation by exhibiting companies. All of our staff sincerely look forward to your visit. Event Name: 41st Annual Meeting of the Japanese DDS Society Date: July 17 (Tuesday) - July 18 (Wednesday) Venue: Makuhari Messe International Conference Hall Details: https://procomu.jp/dds2025/ <Exhibiting Company Presentation> July 17 (Tuesday) around 15:28

CPhI Japan Exhibition & Seminar! April 9 (Wednesday) - 11 (Friday) @ Tokyo Big Sight Unchained Labs, Inc. will be exhibiting in the biopharmaceutical zone at CPhI Japan this year as well. Additionally, we will hold a seminar on the second day! Please visit our booth at Tokyo Big Sight. <Exhibitor Seminar> Date and Time: April 10 (Thursday) 13:00-13:30 Venue: 5B "Stability Evaluation of Biopharmaceuticals - Towards a New Era of High-Speed and High-Throughput" *No prior reservation required, free of charge.

Stability testing of proteins and formulations can feel overwhelming when screening large numbers of candidates or conditions. Traditional tools often lack flexibility, provide only partial information, or have low throughput, wasting valuable time. Aunty is the world's fastest and highest throughput protein stability assessment tool. By setting up to 96 samples on a plate, Aunty processes thermal denaturation and aggregation tests at an astonishing speed. Using fluorescence measurements, SLS (Static Light Scattering), and DLS (Dynamic Light Scattering), it analyzes the entire plate every minute, allowing for instant access to detailed stability data. You can screen large numbers of candidate molecules and conditions and repeat experiments as many times as needed. Aunty delivers unprecedented detailed stability assessments with overwhelming speed and data resolution. In this webinar, we will provide a detailed explanation of the flow from experimental setup to data analysis with Aunty. You will see the analysis of Tm (melting temperature), Tagg (aggregation temperature), and colloidal stability, and experience high-resolution stability data like never before!

We have launched a new product. Please check the link below for details.