ユニーテック List of Products and Services

We offer contract testing services such as "cell surface marker analysis," "multiplex cytokine measurement," "cell cycle analysis," and "apoptosis analysis" using flow cytometry. In addition to measurements using samples sent to us, we can also conduct measurements after culturing at our facility, as well as measurements combined with animal experiments. Furthermore, we provide "cell sorting," which allows for the recovery of target cells from a population composed of multiple cells, such as blood-derived cells and primary cells. 【Representative Analyses (Partial)】 ■ Cell Cycle ■ Apoptosis ■ Cell Surface Antigens (e.g., CD Markers) ■ Immune Cell Evaluation (Lymphocyte Subset Analysis) ■ Intracellular Cytokine Analysis *For more details, please refer to the PDF document or feel free to contact us.

• Cell-related contract
• Other Testing Contract

This catalog focuses on "Genome Editing CRISPR/Cas9 Special Feature" and includes various genome editing technologies. We also introduce topics such as "from monoclonal antibody production to variable region analysis and recombinant antibody production." Additionally, we provide an overview of our contract services, making it a useful reference when considering options. 【Contents (Excerpt)】 ■ Genome Editing CRISPR/Cas9 Special Feature "Mouse Edition" ■ Genome Editing CRISPR/Cas9 Special Feature "Cell Edition" ■ Variable Region Analysis, Expression Vector Construction, Recombinant Antibody Production ■ From Monoclonal Antibody Production to Recombinant Antibody Production and Expression ■ Sales of LB Agar Plates and LB Liquid Medium Production *For more details, please refer to the PDF document or feel free to contact us.

• Cell-related contract

MEF (Mouse Embryonic Fibroblast)" is commonly used as feeder cells and is suitable for maintaining the state of ES/iPS cells. Our MEF has been treated with mitomycin C, so it is ready for immediate use. If you have any requests for changes to vials, please contact us. We also offer "Mouse ES Cell Total RNA" extracted and purified from ES cells cultured without feeder cells (derived from C57BL/6) and "Mouse iPS Cell Total RNA" extracted and purified from mouse iPS cells (derived from C57BL/6). 【Features of Feeder Cells for ES/iPS Cells (MEF)】 ■ Widely used as feeder cells for ES/iPS cells ■ Ready for immediate culture as feeder cells due to mitomycin C treatment ■ Prepared from 12-day-old mouse embryos ■ Cells used are from the third passage ■ Culture tests using mouse ES cells and mycoplasma testing are conducted for all lots *For more details, please refer to the PDF document or feel free to contact us.

• Animal cells

The "LB Agar Medium Plates and LB Liquid Medium" are pre-prepared media used for the selection and proliferation of Escherichia coli strains with various antibiotic resistances and Escherichia coli strains that carry antibiotic resistance markers on plasmids. We offer a wide range of options, from media containing ampicillin to custom-made LB media with additives. When ordering custom LB media, please let us know your desired antibiotics (multiple options are allowed) along with their concentrations. If you have other additive requests, please contact us. 【Features of LB Agar Medium Plates】 ■ Composition - Tryptone 1.0% - Yeast Extract 0.5% - NaCl 1.0% - Agar 1.5% ■ Storage 2°C to 8°C; light protection may be necessary depending on additives. *For more details, please refer to the PDF document or feel free to contact us.

• Culture medium

We would like to introduce the "Fibrosis Model" that we handle. Fibrosis caused by inflammatory responses is an important theme in treatment, but the detailed mechanisms and effective strategies have not yet been established. Our company is capable of producing model animals tailored to specific purposes such as elucidating mechanisms and evaluating drug efficacy, thanks to our experienced staff who can respond to a wide variety of induction conditions. 【Model Examples】 ■ Bleomycin-induced pulmonary fibrosis model ■ Thioacetamide-induced liver fibrosis model *For more details, please refer to the PDF materials or feel free to contact us.

• Animal cells

We would like to introduce the production of model mice/rats for "graft-versus-host disease (GvHD)" that we are conducting. The "acute GvHD model," which is a dangerous complication in bone marrow transplantation, is created by transplanting donor spleen cells into recipient mice, while the "chronic GvHD model" induces symptoms similar to lupus nephritis. Please feel free to contact us for more details. 【Model Examples】 ■ Acute GvHD Model ■ Chronic GvHD Model *For more information, please refer to the PDF document or feel free to contact us.

• Animal cells

We would like to introduce our "Anti-Tumor Evaluation" conducted at our company. Animal experiments are carried out under an environment that considers the spirit of animal welfare, with advanced techniques and thorough breeding management. It is also possible to conduct experiments using other cell lines or provided cell lines. 【Example of Pancreatic Cancer Evaluation】 ■ Animal Used: Nude Mice (8 weeks old, male, Japan Clea) ■ Cells: Human Pancreatic Cancer (BxPC-3, Panc-1) ■ Transplantation: Pancreas ■ Dissection: 42 days after transplantation, 56 days after transplantation *For more details, please refer to the PDF document or feel free to contact us.

• Animal cells

The "Multiple Sclerosis Model" is a central nervous system disease characterized by widespread inflammatory demyelination in the brain and spinal cord. Since paralysis occurs in the hind limbs and other areas, it is important to provide appropriate care, as even slight differences in technique can lead to varying experimental results. At Unitec, we are capable of producing and evaluating high-quality "Multiple Sclerosis Disease Models" through skilled techniques and advanced breeding management. [Features] - Appropriate care is essential due to paralysis in the hind limbs and other areas. - Experimental results can differ with slight variations in technique. - Skilled techniques. - Advanced breeding management. - Capable of producing and evaluating high-quality multiple sclerosis disease models. *For more details, please refer to the PDF materials or feel free to contact us.

• Animal cells

We would like to introduce the "muscle disease models" that we handle. In the "muscle disease models," where muscle weakness is the main symptom, it is important not only to create the models but also to provide appropriate care and evaluation for the symptoms. At our company, we are able to produce and evaluate high-quality models through advanced breeding management techniques and meticulous responses that take the pathological conditions into account. [Model Examples] ■ Muscular Dystrophy Model ■ Myasthenia Gravis Model *For more details, please refer to the PDF materials or feel free to contact us.

• Animal cells

We would like to introduce the "Arthritis Model" that we handle. In the production of autoimmune disease models, stress-free breeding under advanced microbiological control is essential for system stability. Furthermore, providing appropriate care for symptoms is also important for maintaining the health of the mice. Our company is capable of producing high-quality autoimmune disease models through advanced breeding management techniques and meticulous attention to the disease state. [Features] ■ Advanced breeding management techniques ■ Meticulous attention to the disease state ■ Capability to produce high-quality autoimmune disease models *For more details, please refer to the PDF materials or feel free to contact us.

• Animal cells

We would like to introduce the "DNA Methylation Analysis (Bisulfite Sequencing)" offered by Unitec Co., Ltd. We analyze the methylation rates of CpG sites in specific genomic DNA regions from sequences obtained through sequencing. DNA methylation plays a crucial role in gene regulation and is said to be associated with various diseases and disorders. Please leave the design to us. We will design and synthesize primers from the CpG sequences of specific genomic regions. 【Features】 ■ Leave the design to us ■ Primer design and synthesis from CpG sequences of specific genomic regions ■ Proven track record of completing the process within two weeks from sample receipt to report delivery ■ Consultation available for those in a hurry for conferences or publications *For more details, please refer to the PDF document or feel free to contact us.

• Other analysis and sequence

We would like to introduce "Real-time PCR Analysis of Specific Gene Expression" offered by Unitec Co., Ltd. In addition to obtaining experimental data, statistical analysis is also conducted. We accept analyses either in combination with our contracted items or as standalone services. You can choose the desired workflow according to your testing objectives. We offer special prices for the analysis of large samples, additional analyses with the same content, and regular analyses. 【Workflow】 ■Step 0 Experiment ■Step 1 Tissue Collection ■Step 2 Total RNA Extraction and Purification ■Step 3 cDNA Synthesis ■Step 4 Real-time PCR Analysis *For more details, please refer to the PDF document or feel free to contact us.

• Gene expression analysis contract

We would like to introduce the "Vector Construction Contract Service" offered by Unitec Co., Ltd. Our company brings together experienced researchers in dry (informatic) and wet (from experimental planning to execution) methods. As an example of the testing process, the workflow includes meetings, creating design diagrams, vector construction, quality confirmation, and scaling up. You can request services from any step. 【Contract Achievements】 ■ Construction of vectors containing difficult repetitive sequences (similar sequences) ■ Construction of challenging vectors over 20kbp ■ Construction of dual expression vectors (co-expression of gene A and gene B) ■ Simultaneous construction of over 50 specially designed vectors *For more details, please refer to the PDF materials or feel free to contact us.

• Other DNA/RNA

We would like to introduce the "Knock-in Mouse Production" offered by Unitec Co., Ltd. It is possible to create disease model mice by introducing disease-causing SNPs into mice. We can produce mice for in vivo localization and dynamics visualization of target proteins, as well as mice that express human target proteins within their bodies. As additional options, we offer increased targeting vector supply and the production of linked embryos (200 units). We also accept requests for the production of transgenic mice. 【KI Mouse Production Process (Excerpt)】 ■ Design and construction of targeting vectors ■ Establishment of homologous recombination ES cells (derived from C57BL/6J) ■ Production of chimeric mice ■ Production of F1 heterozygous mice ■ Production of Neo sequence removal mice *For more details, please refer to the PDF document or feel free to contact us.

• Other contract manufacturing

We would like to introduce the "Contract Production of Recombinant Retrovirus and Adeno-Associated Virus" handled by Unitec Co., Ltd. Retroviruses integrate into the host cell's genome and express. They are suitable for establishing iPS cells and introducing genes into primary cultured cells. Adeno-associated viruses can also achieve long-term and large-scale transient expression. Due to their almost non-pathogenic nature, experiments can be conducted at BSL1, which is an advantage. 【Features】 ■ Integrates into the host cell's genome and expresses ■ Suitable for establishing iPS cells and introducing genes into primary cultured cells ■ Adeno-associated viruses can also achieve long-term and large-scale transient expression ■ Advantage of being able to conduct experiments at BSL1 ■ Lentivirus production is also possible *For more details, please refer to the PDF materials or feel free to contact us.

• Gene expression analysis contract

We would like to introduce the "Gene-Modified Cell Line Production Service" offered by Unitec Co., Ltd. We carry out the production of cell lines with knockout or knock-in of target genes in cancer cell lines and iPS-derived cells. Our researchers will accompany you or meet with you online to propose methods that suit your objectives. We produce gene-modified cells from various cell types, including HEK293 cells, liver cancer-derived cell lines, lymphoblastoid cell lines, and osteoblast cell lines. 【Features】 ■ Researchers accompany you or meet with you online for direct discussions ■ Production of gene-modified cells from various cell types ■ Successful cases with cells that have difficulties in culture, such as those that cannot undergo limiting dilution ■ Possibility of pharmacological efficacy testing and large-scale stock *For more details, please refer to the PDF document or feel free to contact us.

• Other cells

We would like to introduce "Establishment, Culture, and Differentiation Induction of iPS Cells" handled by Unitec Co., Ltd. Feeder-free culture uses an episomal vector for the production of human iPS cells. It is important to produce high-quality iPS cells, as this affects the quality of the cells after differentiation. The expression of undifferentiated markers is confirmed through flow cytometry analysis and immunofluorescence staining, and verification is also conducted through embryoid body formation. 【Features】 ■ Feeder-free culture ■ Use of episomal vector ■ Impact on the quality of cells after differentiation ■ Confirmation of undifferentiated marker expression ■ Verification through embryoid body formation *For more details, please refer to the PDF materials or feel free to contact us.

• Cell culture contract service

We would like to introduce "Protein Expression and Purification Services Using Animal Cells" offered by Unitec Co., Ltd. From expression vector construction to large-scale culture and purification, we guarantee the final yield through trial culture and purification. Transient protein expression is suitable for those who need a high yield of the desired protein in a relatively short period of time (cultures up to about 1L). It is also possible to express large molecular weight proteins and form complexes through the simultaneous expression of multiple proteins. Additionally, we accept requests for the creation of stable expression cell lines. 【Features】 ■ Capable of expression vector construction, large-scale culture, and purification ■ Final yield guaranteed through trial culture and purification ■ Formation of complexes through the simultaneous expression of multiple proteins is possible ■ Capable of expressing and purifying small amounts of mammalian-type proteins *For more details, please refer to the PDF document or feel free to contact us.

• Other Culture

We would like to introduce "Insect Cell Culture and Purification Using Recombinant Baculovirus" handled by Unitec Co., Ltd. If you are looking for proteins that are closer to eukaryotic organisms, or if you have expressed your target protein in E. coli but were unable to obtain it due to insolubility, why not try expression in insect cells? Trial purification can be completed in as little as two months. Please feel free to consult with us if you have any requests. 【Culture and Protein Purification Using Recombinant Baculovirus】 - Vector construction, baculovirus production, trial culture, and purification culture are all possible. - The expression levels significantly exceed those of animal cells, allowing for the acquisition of proteins with modifications similar to those of animal cells. - Baculovirus cannot infect vertebrates, so it can be handled at BSL1. *For more details, please refer to the PDF materials or feel free to contact us.

• Cell culture contract service

We would like to introduce the "E. coli Cultivation and Purification" handled by Unitec Co., Ltd. We can accommodate your desired cultivation volume, from a single Sakaguchi flask to fermenter cultivation. We propose suitable testing methods for those who wish to conduct large-scale cultivation and purification after considering the conditions. Our company has extensive experience in constructing expression vectors and protein expression. We accept protein expression under various conditions, including E. coli, yeast, insect cells, and animal cells. 【Cultivation and Protein Purification Using Recombinant E. coli】 - Optimization for codon usage (converting the nucleotide sequence to one that is easier to express in E. coli while keeping the amino acid sequence unchanged) - Exploration of induction conditions and cultivation conditions that facilitate more soluble expression - Refolding of insoluble fraction (inclusion body) expressed proteins is also possible - Estimated expression amount: 1–10 mg/L *For more details, please refer to the PDF materials or feel free to contact us.

• Cell culture contract service

Our company offers in vitro pharmacological studies, including activity measurement and toxicity evaluation of various drugs (such as pharmaceutical candidate compounds) using various cell lines. In addition to the dozens of human cancer cell lines we possess, screening via MTS assay is also possible using cells provided by you. We also conduct pharmacological studies on iPS cells and cells differentiated from iPS cells. You can utilize our services for various purposes, including anticancer drug proliferation inhibition tests on cancer cell lines, impact assessments on normal cell lines, cytotoxicity tests, and determination of EC50/IC50 for various test substances. Please feel free to consult us with your requests. *For more details, please do not hesitate to contact us.*

• Other Testing Contract