サーモフィッシャーサイエンティフィック株式会社／Thermo Fisher Scientific K.K. Product Lineup

In the quality control of plating solutions, the quantification of additives is an important factor. In particular, the quantification of sulfur-containing compounds that contribute to film quality adjustment and prevention of liquid decomposition is essential. This document provides accurate and highly reproducible analytical results using a method that does not require complex pretreatment. For the HPLC measurement conditions, an ion-pair reagent was added to the mobile phase, and an injection method was adopted to reduce the influence of sample impurities. As a result, sharp peaks can be obtained without being affected by metals and inorganic ions. Additionally, by comparing the chromatograms of the standard solution and the plating solution sample, it can be confirmed that the concentration of additives is maintained consistently. Furthermore, as an evaluation of reproducibility, the same sample was measured five times, demonstrating high reproducibility. 【Features】 ■ High-sensitivity quantitative analysis without complex pretreatment ■ High reproducibility using ion-pair reagents ■ Injection method that reduces the influence of metals and inorganic ions ■ Accurate quantification through comparison of standard solutions and actual samples ■ Highly reproducible analytical results

This application note introduces the analysis of polymer compounds using size exclusion chromatography (SEC) with the Vanquish Charged-Aerosol Detector (CAD). The CAD has approximately 100 times the sensitivity compared to a differential refractive index detector (RI) and is a versatile detector capable of accurately detecting semi-volatile and non-volatile components. Its wide dynamic range and excellent stability make it ideal for analyzing molecular weight distributions. This document discusses the detection of calibration standard samples and calibration curves, comparisons of sensitivity, and changes in retention time due to sample concentration, using polystyrene samples to compare RI and explain the characteristics and advantages of CAD. 【Features】 ■ High sensitivity detection: CAD achieves approximately 100 times the sensitivity compared to RI ■ Wide dynamic range: Accurately measures samples of diverse molecular weights ■ Detection in the polymer range: Effectively detects polymers with molecular weights up to 400,000 ■ Low concentration measurement: Minimal concerns about carryover or column loading even at low concentrations ■ Stability: Provides rapid stabilization and consistent results

The HPLC Charged Aerosol Detector (CAD) system is a solution that allows for the rapid and highly accurate quantification of lipids in liposome formulations. This technical sample presents the results of validation using the ASTM method across 12 system configurations at 4 sites (Thermo Scientific Vanquish Flex UHPLC and Vanquish Core HPLC). Reliable results were obtained across all systems, and they passed the system suitability tests. Additionally, excellent separation performance was confirmed using the Thermo Scientific Hypersil GOLD column. 【Features】 ■ High precision quantification: Quantification is possible in the range of 0.2 to 300 µg/g using linear, quadratic, and log-log calibration curves. ■ High reliability: Passed system suitability tests at all sites. ■ Excellent performance: High separation capability and rapid analysis with the Hypersil GOLD column. ■ Efficient analysis: Consistent responsiveness and high-precision results obtained quickly.

The Thermo Scientific Vanquish Fraction Collector (VFC) is a system that enables peak-triggered fractionation using UV, CAD, and MS detectors. With CAD and MS detection, it has become possible to fractionate and evaluate unknown components and impurities that were difficult to detect with conventional UV detection. This application note explains the features of the VFC equipped with innovative valve technology, using examples from plant extracts. It provides detailed practical know-how from parameter settings for accurate fractionation to applications on a semi-preparative scale. 【Features】 ■ Low contamination: Cleaning functions for needle and buffer loop ■ High precision fractionation: Automatic calculation of delay volume, peak-triggered fractionation ■ Excellent versatility: Diverse fractionation settings using UV, CAD, and MS detection ■ High reliability: Fractionation enabled by innovative valve technology

This application note presents a case study of the high-precision separation and collection of cis/trans isomers of vitamin K1 using the Thermo Scientific Vanquish Core HPLC system and Vanquish Fraction Collector. The normal phase kit extends compatibility with organic solvents such as hexane, confirming excellent reproducibility that meets EP standards (RSD < 1%). Recovery rates of 99.5% to 101.4% were achieved for both peak-based and time-based collections. Furthermore, collection and storage were conducted under temperature control, comparing isomerization behavior at 8 °C and 35 °C, demonstrating that temperature management is crucial for suppressing structural changes. For more details on reliable isomer purification using the normal phase compatible collection system, please refer to the materials. 【Features】 ■ Extended solvent compatibility with normal phase HPLC compatible kit ■ High reproducibility with retention time and peak area RSD < 1% ■ High-precision collection with recovery rates of 99.5% to 101.4% ■ Significant suppression of isomerization with low-temperature storage at 8 °C ■ Quantitative evaluation of isomerization reactions and verification of temperature dependence

This application note presents a case study of transferring the purification and analysis workflow of synthetic oligonucleotides from a competitor's HPLC system to the Thermo Scientific Vanquish HPLC system. Focusing on dual-labeled oligonucleotide samples, the workflow was validated through purification via semi-preparative RP-HPLC, purity assessment using HPLC-UV, and identity confirmation with HRAM-MS. A comparison using the Hypersil GOLD column demonstrated the ability to isolate the target oligonucleotides with high purity, achieving efficient quality control. The note provides practical comparative data on retention behavior, peak shape, and collection capacity differences during method transfer. **Features** - Case study on the transfer from a competitor's LC purification system to the Vanquish analytical purification LC system. - Validation of purification, purity assessment, and identity confirmation of dual-labeled oligonucleotides as a complete workflow. - Comparison of separation performance between the Hypersil GOLD column and competitor columns. - Explanation of differences in peak shape and collection capacity with specific chromatograms. - Introduction of an efficient quality control method combining HPLC-UV and HRAM-MS.

This application note introduces amino acid analysis utilizing the automatic derivatization feature of the Thermo Scientific Vanquish Flex UHPLC system. Online chiral derivatization using OPA/NAC was employed to conduct quantitative analysis of D-aspartic acid (D-Asp), D-glutamic acid (D-Glu), and D-serine (D-Ser). By automating the process from derivatization to injection within the auto-sampler, we streamline complex sample preparation while achieving high reproducibility and quantitativeness. Furthermore, we demonstrate the quantification of D-amino acids present in fermented foods such as apple cider vinegar, grain vinegar, and whey, showing that sensitive and selective analysis is possible even in complex sample matrices. **Features** - Separation of D and L forms of eight amino acids including D-Asp, D-Glu, and D-Ser in under 15 minutes. - High reproducibility confirmed with retention time RSD ≦ 0.16% and area value RSD ≦ 0.22%. - Good linearity in the calibration range of 1–500 ng/mL (R² ≧ 0.9999). - Examples of D-amino acid quantification in fermented foods (apple cider vinegar, grain vinegar, whey) along with chromatograms are presented.