プロテインウエーブ List of Products and Services

During the loop-out of protein crystals, the mother liquor excessively remains within the loop due to surface tension. This mother liquor exerts compressive stress on the crystals during freezing, degrading their crystallinity. Therefore, reducing the amount of mother liquor improves the resolution of the crystals. "ActiLoops" is a protein crystal mounting and measurement tool designed to hydrophilize the surfaces of the loop, groove, and reservoir, allowing the excess mother liquor remaining in the loop to be naturally discharged into the reservoir through the groove. Please feel free to contact us if you have any inquiries. 【Features】 ■ High-resolution X-ray diffraction data collection (reducing stress on the crystals during freezing) ■ Reduction of X-ray background from the material (same material as LithoLoops) *For more details, please download the PDF or feel free to contact us.

• Other measurement, recording and measuring instruments

"SPICA" is a microtube for X-ray diffraction measurement made from a special polyimide resin (API). We offer three types of products for all sizes of tubes: tubes only, tubes with sealed bottoms, and tubes with sealed bottoms and a funnel attached to the top. By controlling the microfibril structure of the polyimide polymer, the background (BKG) during X-ray irradiation is significantly reduced. 【Features】 ■ Material: API (polyimide resin) ■ Heat resistance temperature: API resin 350℃ ■ Maximum usage temperature: SPICA 110℃ ■ Tube thickness: 25um ■ Chemical stability: Insoluble to acids, alkalis, and organic solvents *For more details, please refer to the PDF document or feel free to contact us.

• tube

Our company is advancing the understanding of disease mechanisms and the development of preventive methods, as well as preventive and functional foods, based on the results of protein science research. So far, we have successfully purified beta-secretase as a recombinant protein in large quantities and high purity, and we have conducted a search for inhibitory candidate substances using this enzyme. As a result, we found that only one type of natural product, which is present in trace amounts in plants, shows a clear inhibitory effect on beta-secretase. Additionally, since the natural product we discovered has a low content of about 20%, we have been investigating cultivation methods to increase the content over many years, and we have been able to raise it to about 50%. *For more details, please download the PDF or feel free to contact us.

• Public Testing/Laboratory

We accept orders for custom-made protein crystal mounts and measurement tools called "LithoLoops." We design and manufacture loops in the sizes and shapes desired by our customers. To reduce production costs, we recommend placing orders in a foundry format. Additionally, the material is the same as the standard "LithoLoops," and the standard delivery time is approximately 6 weeks. Please feel free to contact us if you have any requests. [Overview] ■ Custom specifications ■ Material is the same as the standard "LithoLoops" ■ Standard delivery time is approximately 6 weeks ■ Design of loop patterns and structural strength, calculating suitable thickness and shape *For more details, please download the PDF or feel free to contact us.

• Other measurement, recording and measuring instruments

"LithoLoops-Viscous" is a protein crystal mounting and measurement tool that makes it easy to immerse loops into the mother liquor and selectively extract the desired crystals. The material of the loops is the same as "LithoLoops," and we offer three sizes: 0.1mm, 0.2mm, and 0.3mm. 【Specifications】 ■ Film thickness: 45 microns ■ Loop width (WL): 35 microns ■ Loop shape: Circular ■ Neck length (LN): 0.85mm ■ Neck connection length (LIN): 0.25mm ■ Minimum neck width (WN) for 0.2mm diameter: 0.24mm *For more details, please download the PDF or feel free to contact us.

• Other measurement, recording and measuring instruments

During the loop-out of protein crystals, the mother liquor excessively remains within the loop due to surface tension. This mother liquor applies compressive stress to the crystals during freezing, degrading their crystallinity; therefore, reducing the amount of mother liquor improves the resolution of the crystals. 'ActiLoops' is a protein crystal mounting and measurement tool designed to hydrophilize the surfaces of the loop, groove, and reservoir, allowing the excess mother liquor remaining in the loop to be naturally discharged into the reservoir through the groove. Please feel free to contact us if you have any inquiries. 【Features】 ■ High-resolution X-ray diffraction data collection (reducing stress on the crystals during freezing) ■ Reduction of X-ray background from the material (same material as LithoLoops) *For more details, please download the PDF or feel free to contact us.

• Other measurement, recording and measuring instruments

At Protein Wave Co., Ltd., as the company name suggests, we focus on drug development (drug design) centered around proteins and provide essential research and development services to unravel the mysteries of life phenomena. We also offer contract services such as biophysical methods/tools and X-ray crystallography of proteins. We can handle individual tasks or provide comprehensive services from expression to crystallographic analysis. Additionally, we provide regular and irregular progress reports, business reports, and data submissions. 【Features】 ■ Target proteins ■ Construction of expression vectors and large-scale culture ■ Protein purification ■ Crystallization/co-crystallization ■ Collection of X-ray diffraction data ■ Data processing ■ Structural analysis *For more details, please refer to the PDF materials or feel free to contact us.

• Other contract services

◆ MOIST is a tool for X-ray diffraction measurements of protein crystals at room temperature, combining our LithoLoops and SPIKA (polyimide tube). ◆ There is no background X-ray generated due to the materials of the loop and polyimide tube. ◆ The airtightness within the tube is good, and the mother liquor or crystals retained in the loop do not dry out for over a month under conditions of 20°C and 30-50% RH. ◆ Soaking compounds into protein crystals or replacing with antifreeze agents can be easily performed while mounted. ◆ The CryoCaps can be recycled after use.

• Other laboratory equipment and containers
• Other measurement, recording and measuring instruments
• Other polymer materials

SPIKA is a microtube for X-ray diffraction measurements made from a special polyimide resin. By controlling the microfibril structure of the polyimide polymer, the background (BKG) during X-ray irradiation is significantly reduced. ◇ Applications: Powder X-ray diffraction, single crystal X-ray diffraction, neutron diffraction ◇ Heat resistance temperature: 300°C ◇ Chemical stability: Insoluble to acids, alkalis, and organic solvents ◇ Grades: Three types: low BKG type ST, SP, and BKG-free LB ◇ Tube inner diameters: 0.3mm, 0.8mm, 1.0mm, 2.0mm, 3.0mm, 7.0mm ◇ Tube lengths: Standard length is 4.0mm, can be manufactured up to a maximum of 80mm ◇ Shape: For diameters other than 0.3mm, options for with/without funnel are available ◇ Tube sealing: Standard products are sealed on one side ◇ Advantages: 　・The resin colors are light yellow for ST, transparent for SP, and light brown for LB, making it easy to observe samples inside the tube 　・Compared to commercially available thermosetting polyimide resins, the X-ray BKG is significantly reduced 　・Safe experiments can be conducted without the risk of breakage like glass materials

• Other laboratory equipment and containers
• Analytical Equipment and Devices

- The material is made of polyimide, and a significant feature is that it does not generate any X-ray background compared to off-the-shelf products. - It is possible to manufacture loops ranging from tiny loops with diameters of several tens of microns to large-diameter loops of several tens of millimeters. - The bending strength of the main body is guaranteed through product design based on structural design. - Loops of arbitrary shapes (oval, mesh, polygon, etc.) can be produced. - In addition to catalog products, we accept design and manufacturing of special specifications (custom products). - The hydrophobicity of polyimide film is lower compared to other resins, allowing for a reduction in the amount of mother liquor during crystal extraction. - It has excellent chemical resistance to acids, alkalis, inorganic salts, and organic solvents contained in crystallization reagents. - Since no fluorescence is generated when ultraviolet laser is irradiated on the crystal, alignment work of the crystal can be easily performed. - There are many usage records at synchrotron facilities both domestically and internationally. - Domestic sales agent: Wakenbi Tech Co., Ltd.

• Other protein analysis

This is an optimal cryoloop for mounting and X-ray diffraction measurements of microcrystals during protein structure analysis. 【Features】 ◆ Due to its hydrophilicity, it does not retain excess moisture when extracting crystals. ◆ It can create loop diameters of less than 0.04 mm, which is impossible with nylon loops, and can be made in any shape, making it ideal for mounting and measuring microcrystals and elongated columnar crystals. ◆ The loop diameter is manufactured with precision. ◆ The hydrophilicity of the polyimide film material is higher than that of nylon, resulting in a reduced amount of mother liquor during loop-out. This helps to lower the background from the mother liquor. ◆ It is compatible with high-throughput screening and high-quality crystallization. ◆ The loop diameter size is displayed on the loop body, making identification easy.

• Other measurement, recording and measuring instruments

To produce high-quality, high-resolution protein crystals, we utilize the effects of electric fields to create protein crystal nuclei under low supersaturation conditions, and we also provide easy-to-use horizontal gel crystallization devices. 【Features】 ◆ A novel crystallization device that utilizes dielectrophoresis for producing high-resolution protein crystals, along with a dedicated power supply for operation. The maximum driving voltage at a frequency of 1 MHz is approximately 28V (AC). ◆ Unlike conventional vertical gel diffusion crystallization devices that use capillaries, our design places the precipitant and protein solution facing each other in the plane through a gel layer, making the dispensing operation and retrieval of grown crystals very simple. ◆ To semi-automate the seed crystallization method, the discharge of the protein solution and the precipitant solution can be performed semi-automatically. This eliminates the need to remove the grown crystals from the wells, preventing damage to the crystals.

• Other measurement, recording and measuring instruments