Announcement of the launch of new products in the UpCellⓇ series (flask and insert)

No need for trypsin! Macrophages and others are collected intact. The temperature-responsive culture equipment UpCell has "grid walls" arranged at 3mm intervals.

◆The temperature-responsive culture device UpCell for cell recovery is equipped with "grid walls" placed at 3mm intervals. ◆It has become easier to recover cultured cells in single cell/small colony form. ◆The grid wall is a new technology that prevents cells from proliferating beyond the grid by using optimized grooves on the surface of the culture dish. Cells can be recovered intact with just a short time and temperature treatment. Advantages of RepCell - It is known that trypsin significantly damages cell viability, but with RepCell, it is possible to recover cells while maintaining surface antigens and extracellular matrix. - Cell detachment using only temperature treatment The only necessary procedure for cell recovery is temperature treatment, eliminating the need for cumbersome pipetting operations associated with traditional trypsin use. For more details, please contact us or refer to the catalog.

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UpCell dedicated cell sheet stacking device. Stacking of sheets becomes easy. Demo unit available.

Features of CellStamp ● Utilizes the adhesion of gelatin gel and cell sheets for layering * ● Can be sterilized by autoclaving ** ● Compatible with UpCell 3.5 cm dishes and 24-well multi-wells

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Live demonstration available! While culturing cells, we will measure the TER (transepithelial/endothelial resistance) and Ccl (capacitance) of the cell layer in real-time.

Features 1) Measurement possible while culturing cells: Long-term measurements can be conducted with the cell module placed inside the incubator. 2) Medium exchange and addition of reagents possible: The module can be removed from the incubator for operations. 3) Compatible with cell culture inserts from various manufacturers: Provides optimized medium volume for each company's inserts. 4) Each electrode is independent: Measurement of up to 24 samples is possible. (+/2) Cleaning the electrodes is easy, and inserts of different sizes can also be used. Specification of the electrodes to be measured is possible. Demonstrations are available. For more details, please contact us or refer to the catalog. You can also watch an introduction video of cellZscope on our YouTube channel.

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At our cell culture center, we conduct contract services related to cell sheet manufacturing, facility operation, and the preparation of application documents.

Features of Regenerative Medicine Contract Services 1. Development and Contract Manufacturing of Cell Sheet Products We provide contract development and manufacturing services primarily for cell sheets, commissioned by pharmaceutical companies and research institutions. Our team includes clinical culture specialists certified by the Japanese Society for Regenerative Medicine, and we offer high-quality cell sheet product development and manufacturing using experienced staff in a cell culture processing facility licensed for the production of specific cell processed products. 2. Facility Management and Application Support The "Act on Securing Safety for Regenerative Medicine" stipulates the structure, equipment, and operation of cell processing, requiring each cell culture processing facility to submit notifications and applications for permits to the Minister of Health, Labour and Welfare. At CellSeed, we assist with the notification and application documentation for the manufacture of specific cell processed products, as well as the creation of necessary operational documents. Additionally, we support the necessary administrative procedures, including the preparation of consultation materials for authorities regarding applications such as clinical trial notifications. 3. Education for Cell Culture Technicians We conduct training for cell culture technicians who have no prior experience with cell sheets, covering procedures such as cell sheet culture and detachment at our cell culture facility. For more details, please contact us or refer to our catalog.

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Live demonstration available! The difference compared to metal heating plates is clear!

It is a transparent heating element made of specially processed hard glass. It improves work efficiency in clean benches for tasks such as microscope observation and media exchange using UpCell/RepCell. Temperature settings are possible from room temperature to 50°C. It is lightweight (approximately 70g) and suitable for portability. It can also be used to cover petri dishes and multi-well plates to prevent fogging during photography. Demonstrations are available. For more details, please contact us or refer to the catalog.

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It is possible to collect mesenchymal stem cells, dendritic cells, macrophages, and cell sheets without using trypsin and without causing damage.

- Celsid's Technology By utilizing a unique nano-surface design, temperature-responsive polymer (PIPAAm) has been immobilized on the surface of the equipment. This allows the surface to change from hydrophobic (cell-adhesive surface) to hydrophilic (cell-releasing surface) by lowering the surface temperature around 32°C. Without using any enzymes that can damage cells, such as trypsin, intact cells can be recovered simply by setting the equipment temperature to 20-25°C and waiting for about 10-30 minutes. - Benefits 1) Easy cell recovery through temperature control. 2) Recovered cells completely retain the extracellular matrix. 3) No need for trypsin, which can harm cells. 4) Suitable for culturing tissues used in regenerative medicine research. 5) For 3D culture by stacking homo/heterogeneous cell sheets. For more details, please contact us or refer to the catalog.

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Complete suppression of cell adhesion in cell culture.

By applying our unique nano-surface design technology, we have fixed (covalently bonded) superhydrophilic polymers to the surfaces of our equipment. In addition to being superhydrophilic, this polymer creates a free water film. The double effect of "superhydrophilicity" and "free water" has achieved a level of low adhesion performance that far surpasses conventional low-adhesion equipment. For more details, please contact us or refer to our catalog.

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Sample presentation available! Cell sheets can be easily collected and transplanted. We use materials that are gentle on cells.

Features Dedicated cell sheet recovery support "CellShifter" for UpCell Utilizes a material with flexibility and water absorption suitable for cell detachment that could not be achieved with hydrophilic PVDF membranes or nitrocellulose membranes. This allows for the reliable and easy recovery of your precious cell sheets that have been carefully cultured with UpCell. The CellShifter, with the cell sheet adhered to it, boasts excellent ease of handling when transplanted directly to the host. Thanks to the specially treated material, there are almost no detached fibers. We guarantee maximum experimental results. Please take this opportunity to experience the results of years of material development.

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We handle TFT LCD modules from WINSTAR for use in display sections of devices. 【We receive inquiries in the following situations】 - Wanting to update the display section of measuring instruments or control panels - Looking for LCDs to incorporate into industrial equipment - Searching for a supplier that can accommodate small quantities 【We can assist with】 - Selecting sizes and interfaces that meet application and enclosure conditions - Confirming pin assignments and arranging samples - Developing custom products and embedded systems - Selling in small lots We also handle OLED (organic EL) modules from the same WINSTAR company. Please consider these as well for devices that want to take advantage of their thinness, low power consumption, and wide viewing angles. Please check the specific specifications in the manufacturer's datasheet. We also accept requests for quotes only.

CLT is known as a building material, but its applications are not limited to just buildings. The wooden lightweight mat "Hyper CLT Mat" is a new option for utilizing CLT in civil engineering. It can be used as a wooden mat in places where steel plates have been the norm, such as temporary roads, access routes, heavy machinery work yards, and material storage areas. The 5×10 mat is relatively light at about 230 kg, making it easy to install, remove, and relocate. Additionally, it offers different values compared to steel, such as reducing glare and metallic noise, as well as CO2 fixation through wood. Moreover, the Hyper CLT Mat is registered with the Ministry of Land, Infrastructure, Transport and Tourism as a NETIS technology "TH-250021-A." It can also be utilized for proposals involving the use of new technologies and environmental considerations in public works. From construction to civil engineering, the new use of wood is expanding in the form of wooden lightweight mats.

Metal cracks are one of the concerns in equipment maintenance operations. Conventional welding methods for repairs can cause changes in the base material around the crack due to heat, leading to residual stress and deformation, which always carries the risk of "crack recurrence" and "secondary damage." Hinode Water Supply Equipment Co., Ltd.'s "Mechanical Stitching Method" is a new technique that allows for the repair of metal cracks without applying heat. By using special bolts and reinforcement plates, the crack is physically removed without affecting the base material at all. Repairs can be made even in environments where open flames cannot be used. Since no heat is applied, the disassembly of equipment is minimized. For more details, please contact us or download the catalog.

We have newly added a **"Frequently Asked Questions Q&A"** section to the Hyper CLT sheet website. It clearly summarizes many questions frequently asked by those considering the introduction of the product, such as "What types of civil engineering sites can it be used on?", "Can it be used on rainy days?", "Is it compatible with large machinery?", and "What is NETIS?". The Hyper CLT sheet is a lightweight wooden sheet utilizing wood CLT, suitable for a wide range of applications including temporary roads, access routes, and heavy machinery work yards. It is also registered as a **Ministry of Land, Infrastructure, Transport and Tourism NETIS registered technology "TH-250021-A"**. Since it is a new material, we will not leave any uncertainties unaddressed. We aim to create a website that makes it easy to resolve questions before introduction, including information on wood, NETIS, civil engineering, and CLT, and we will continue to enhance the Q&A section.

We have added a new **"Glossary"** to the Hyper CLT sheet website. In the civil engineering and construction industries, there are many specialized terms such as "CLT," "NETIS," "temporary roads," and "wooden lightweight sheets." Therefore, we have introduced related terms in an easy-to-understand manner so that those who are encountering Hyper CLT sheets for the first time can understand the products and technologies. Hyper CLT sheets are wooden lightweight sheets for civil engineering that utilize wood CLT and are registered under the Ministry of Land, Infrastructure, Transport and Tourism's NETIS technology as **"TH-250021-A."** We hope that by not only conveying the features of the product but also sharing the underlying technologies and terminology, users will feel a closer connection to Hyper CLT sheets. We will continue to enhance our website and provide clear information related to wood, NETIS, civil engineering, and CLT.