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  3. ファスマック
  4. Long-chain RNA synthesis (mRNA contract synthesis service)
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  • Jan 18, 2022
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Jan 18, 2022

Long-chain RNA synthesis (mRNA contract synthesis service)

ファスマック ファスマック
We will synthesize your desired long-chain RNA using in vitro transcription methods. This can be used for various applications such as RT-PCR amplification control RNA, gRNA for genome editing, small RNA, mRNA preparation, RNA aptamers, etc. The synthesis will be performed via in vitro transcription using T7 polymerase from template DNA (prepared by PCR from plasmid clones or oligo DNA). After silica column purification, we will confirm purity and yield through absorbance measurement and electrophoresis. 【Details】 ■ Length: 50–120 nt ■ Guaranteed delivery amount: 20 μg ■ Template DNA: Synthetic oligo DNA (no additional cost) ■ Delivery time: 10 business days ■ Length: 121–1500 nt ■ Guaranteed delivery amount: 40 μg ■ Template DNA: Artificial gene synthesis (additional cost) ■ Delivery time: 10 business days (after receipt of template DNA) If you have a plasmid clone to use as a template, that can also be utilized. If you have requests for longer RNA, increased delivery amounts, 5' cap, polyA-tail, etc., please feel free to consult with us.
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Long Chain RNA Contract Synthesis
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Long-chain RNA synthesis (mRNA contract synthesis service)

Synthesis of long-chain RNA using in vitro transcription method! Plasmid clones can also be used as templates.

At Fasmac, we offer "long-chain RNA synthesis." We synthesize RNA through in vitro transcription using T7 polymerase from template DNA (prepared from plasmid clones via PCR or oligo DNA), followed by purification with silica columns. The purity and yield are confirmed through absorbance measurement and electrophoresis. Please contact us with your desired sequences for various applications such as RT-PCR amplification control RNA, gRNA for genome editing, small RNA, mRNA preparation, RNA aptamers, etc. 【RNA Usage Examples】 ■ RNA of the GFP gene sequence was synthesized, and after adding a 5' cap and polyA-tail, it was introduced into cultured cells using the lipofection method. ■ Green fluorescence was observed approximately 5 hours after introduction. *For more details, please refer to the PDF materials or feel free to contact us.

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<Research Support> Would you like to solve the challenges you are currently facing together?

We offer a variety of research support applications that allow for approaches tailored to different research stages. Please feel free to contact us for more information.

<Strengths and Features of Fasmac Co., Ltd.> ■ World-class inspection and manufacturing technology ISO17025 certification, ISTA certification, ISO9001 certification ■ Commitment to research and development Standard analysis methods for GM foods, GM seed screening technology, and many others ■ Robust infrastructure and sales channels Various DNA analysis devices, numerous skilled technicians Established sales network to various research institutions <Research and Development Achievements (Partial)> ■ Improvement of genome editing technology CRISPR/Cas method Joint research with Tokyo Medical and Dental University, Hiroshima University, and Keio University ■ Random integration analysis Joint research with the National Institute of Infectious Diseases ■ Molecular quantity guaranteed DNA standard materials Joint research with Ricoh Company, Ltd. ■ Development of a skin resident bacteria detection kit using DNA chromatography methods Joint research with Menard Co., Ltd. *If you have any concerns, please feel free to contact us. We aim to add new value to existing platforms and hope to collaborate with all of you.

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Genome Editing Tool 'GenomeCraft - Donor DNA'

We will introduce ssODN synthesis, ssDNA production, and targeting vector creation!

We would like to introduce our genome editing tool, 'GenomeCraft - Donor DNA.' In "ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis," it can be used as donor DNA to knock in sequences of up to several dozen bases, including base substitutions and loxP sites. In "ssDNA (single-stranded DNA) production," we create long single-stranded DNA that cannot be synthesized using oligodeoxynucleotide synthesis, and in "targeting vector production," we produce donor DNA for gene targeting, such as gene knockout and knock-in. Please feel free to contact us when you need our services. 【Service Details】 ■ ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis ■ ssDNA (single-stranded DNA) production ■ Targeting vector production *For more details, please refer to the PDF document or feel free to contact us.

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Fasmac Inc. Company Profile

The inspection technology is available for everyone not only in Japan but also in the United States, China, and other countries!

At Fasmac, since our establishment in 2001, we have been advancing the development of "Japanese Standard Analytical Methods" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare, utilizing molecular biological techniques. The developed testing technologies are available not only in Japan but also in the United States, China, and other countries. Additionally, since our establishment, we have been actively engaged in activities such as the international standardization of "food inspection methods using molecular biological techniques," and our technical capabilities are internationally recognized. 【Business Activities】 ■ Food inspection and various contract services ■ Manufacturing and sales of food inspection reagents, manufacturing and sales of reagents for genetic engineering *For more details, please refer to the PDF materials or feel free to contact us.

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Pharmaceuticals and Biotechnology
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Hyper CLT flooring panel

Construction industry focuses on 'Civil Engineering x Timber' | Hyper CLT Flooring

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CLT, which has been widely adopted in the construction field, is now expanding its applications into the civil engineering sector. The "Hyper CLT Mats" are civil engineering CLT products that utilize wood CLT as temporary materials. Traditionally, steel plates have been the mainstream choice for temporary roads, access routes, and heavy machinery yards. The Hyper CLT Mats introduce a new option of "wood" to that market. The 5x10 mats weigh approximately 230 kg, making them lightweight and easy to install and remove with small machinery, with a maximum capacity of 40 mats on a 10-ton truck. They also have advantages in terms of workability and transportation efficiency. Moreover, they offer added value that can only be achieved with wood, such as glare reduction, noise control, CO2 fixation, and consideration for aesthetics. This product is expected to be a new proposal for not only construction companies but also building material firms, lumber companies, and material trading companies. It is a registered technology with the Ministry of Land, Infrastructure, Transport and Tourism under NETIS, with the registration number "TH-250021-A." The use of wood is not limited to construction anymore. The utilization of Hyper CLT Mats is expanding as a new market that connects civil engineering and wood.

Aug 22, 2026

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Please let us know your concerns regarding leaks. We may have some suggestions for you. Leak tests, leakage tests, leak inspections, leak detection, container integrity tests, packaging integrity tests...

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Fukuda Corporation has been pursuing leak detection for 60 years and is dedicated to advancing the future of leak testing. 【 Supporting Various Leak Tests: Air Leak Test / Gas Leak Test (Hydrogen Gas, Helium Gas) 】 We develop, manufacture, and sell leak testing equipment to inspect airtightness and sealing performance. We will propose suitable testers and devices for each inspection target. 【 Target Industries and Examples of Inspection Targets 】 - Automotive Industry: Engines, FC components, valves and piping, various parts - Electronic Components Industry: Smartphones, keyless switches, various small sensors - Pharmaceuticals / Food / Cosmetics Industry: Bottle containers, syringes, vials, eye drop containers, pillow packaging, PTP packaging We will propose the most suitable leak testers for each industry. 【 ISO Certification 】JIS Q 9001:2015 (ISO 9001:2015)

Aug 21, 2026

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Fukuda's Technology Column

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This is a collection of links to Fukuda's technical columns. It covers various topics related to leak tests and more... Please check the related links for more information.

Aug 21, 2026

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Hyper CLT flooring panel

Utilized for temporary roads on slopes | Wood CLT "Hyper CLT Paving Slabs"

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In civil engineering sites such as mountainous areas and construction sites, it may be necessary to use wooden boards on slopes or inclined access roads. Hyper CLT boards are civil engineering materials utilizing wood CLT, which can be laid not only on flat ground but also on sloped terrain. They can be used for temporary roads, access routes, heavy machinery entry paths, and measures against muddy conditions, creating a driving environment that takes advantage of the unique surface characteristics of wood. The 5×10 board weighs approximately 230 kg, and the 5×20 board weighs about 460 kg, making them lightweight and easy to install and remove even with small machinery, which is a notable feature for maneuverability in narrow sites. This wooden board is registered as a technology under the Ministry of Land, Infrastructure, Transport and Tourism's NETIS system as "TH-250021-A," and can also be utilized for safety measures and innovative ideas in public works.

Aug 21, 2026

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PX Suction Cup Series for Handling Challenging Surfaces

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Stable adhesion to rough and uneven surfaces such as stone, sheet metal, textured glass, and wood. Piab's "PX Suction Cup Series" is designed for handling rough surfaces, patterned surfaces, and uneven workpieces that are difficult to hold with standard flat suction cups. The high-conformity EPDM sealing ring adapts to the irregularities and shapes of the work surface, while the rigid aluminum support plate and internal support pad provide a stable grip. The internal support pad helps stabilize thin sheets and delicate workpieces while minimizing suction marks. It is suitable for demanding industrial applications involving stone, metal, glass, and wood. Additionally, a cone valve integrated version is also available. By preventing leaks from suction cups that do not contact the workpiece, it enhances safety during transport while reducing air consumption. This is also effective for energy-saving measures in large-area grippers that use multiple suction cups.

Aug 21, 2026

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