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  3. ファスマック
  4. Long-chain RNA synthesis (mRNA contract synthesis service)
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  • Jan 18, 2022
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Jan 18, 2022

Long-chain RNA synthesis (mRNA contract synthesis service)

ファスマック ファスマック
We will synthesize your desired long-chain RNA using in vitro transcription methods. This can be used for various applications such as RT-PCR amplification control RNA, gRNA for genome editing, small RNA, mRNA preparation, RNA aptamers, etc. The synthesis will be performed via in vitro transcription using T7 polymerase from template DNA (prepared by PCR from plasmid clones or oligo DNA). After silica column purification, we will confirm purity and yield through absorbance measurement and electrophoresis. 【Details】 ■ Length: 50–120 nt ■ Guaranteed delivery amount: 20 μg ■ Template DNA: Synthetic oligo DNA (no additional cost) ■ Delivery time: 10 business days ■ Length: 121–1500 nt ■ Guaranteed delivery amount: 40 μg ■ Template DNA: Artificial gene synthesis (additional cost) ■ Delivery time: 10 business days (after receipt of template DNA) If you have a plasmid clone to use as a template, that can also be utilized. If you have requests for longer RNA, increased delivery amounts, 5' cap, polyA-tail, etc., please feel free to consult with us.
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Long Chain RNA Contract Synthesis
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Long-chain RNA synthesis (mRNA contract synthesis service)

Synthesis of long-chain RNA using in vitro transcription method! Plasmid clones can also be used as templates.

At Fasmac, we offer "long-chain RNA synthesis." We synthesize RNA through in vitro transcription using T7 polymerase from template DNA (prepared from plasmid clones via PCR or oligo DNA), followed by purification with silica columns. The purity and yield are confirmed through absorbance measurement and electrophoresis. Please contact us with your desired sequences for various applications such as RT-PCR amplification control RNA, gRNA for genome editing, small RNA, mRNA preparation, RNA aptamers, etc. 【RNA Usage Examples】 ■ RNA of the GFP gene sequence was synthesized, and after adding a 5' cap and polyA-tail, it was introduced into cultured cells using the lipofection method. ■ Green fluorescence was observed approximately 5 hours after introduction. *For more details, please refer to the PDF materials or feel free to contact us.

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<Research Support> Would you like to solve the challenges you are currently facing together?

We offer a variety of research support applications that allow for approaches tailored to different research stages. Please feel free to contact us for more information.

<Strengths and Features of Fasmac Co., Ltd.> ■ World-class inspection and manufacturing technology ISO17025 certification, ISTA certification, ISO9001 certification ■ Commitment to research and development Standard analysis methods for GM foods, GM seed screening technology, and many others ■ Robust infrastructure and sales channels Various DNA analysis devices, numerous skilled technicians Established sales network to various research institutions <Research and Development Achievements (Partial)> ■ Improvement of genome editing technology CRISPR/Cas method Joint research with Tokyo Medical and Dental University, Hiroshima University, and Keio University ■ Random integration analysis Joint research with the National Institute of Infectious Diseases ■ Molecular quantity guaranteed DNA standard materials Joint research with Ricoh Company, Ltd. ■ Development of a skin resident bacteria detection kit using DNA chromatography methods Joint research with Menard Co., Ltd. *If you have any concerns, please feel free to contact us. We aim to add new value to existing platforms and hope to collaborate with all of you.

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Genome Editing Tool 'GenomeCraft - Donor DNA'

We will introduce ssODN synthesis, ssDNA production, and targeting vector creation!

We would like to introduce our genome editing tool, 'GenomeCraft - Donor DNA.' In "ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis," it can be used as donor DNA to knock in sequences of up to several dozen bases, including base substitutions and loxP sites. In "ssDNA (single-stranded DNA) production," we create long single-stranded DNA that cannot be synthesized using oligodeoxynucleotide synthesis, and in "targeting vector production," we produce donor DNA for gene targeting, such as gene knockout and knock-in. Please feel free to contact us when you need our services. 【Service Details】 ■ ssODN (single-stranded oligodeoxynucleotide donor DNA) synthesis ■ ssDNA (single-stranded DNA) production ■ Targeting vector production *For more details, please refer to the PDF document or feel free to contact us.

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Fasmac Inc. Company Profile

The inspection technology is available for everyone not only in Japan but also in the United States, China, and other countries!

At Fasmac, since our establishment in 2001, we have been advancing the development of "Japanese Standard Analytical Methods" for genetically modified foods and food allergens in collaboration with related agencies of the Ministry of Agriculture, Forestry and Fisheries and the Ministry of Health, Labour and Welfare, utilizing molecular biological techniques. The developed testing technologies are available not only in Japan but also in the United States, China, and other countries. Additionally, since our establishment, we have been actively engaged in activities such as the international standardization of "food inspection methods using molecular biological techniques," and our technical capabilities are internationally recognized. 【Business Activities】 ■ Food inspection and various contract services ■ Manufacturing and sales of food inspection reagents, manufacturing and sales of reagents for genetic engineering *For more details, please refer to the PDF materials or feel free to contact us.

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Pharmaceuticals and Biotechnology
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Hyper CLT flooring panel

Quickly resolve your questions | We have added a Q&A page for Hyper CLT flooring.

  • NEW
  • PRODUCT

We have newly added a **"Frequently Asked Questions Q&A"** section to the Hyper CLT sheet website. It clearly summarizes many questions frequently asked by those considering the introduction of the product, such as "What types of civil engineering sites can it be used on?", "Can it be used on rainy days?", "Is it compatible with large machinery?", and "What is NETIS?". The Hyper CLT sheet is a lightweight wooden sheet utilizing wood CLT, suitable for a wide range of applications including temporary roads, access routes, and heavy machinery work yards. It is also registered as a **Ministry of Land, Infrastructure, Transport and Tourism NETIS registered technology "TH-250021-A"**. Since it is a new material, we will not leave any uncertainties unaddressed. We aim to create a website that makes it easy to resolve questions before introduction, including information on wood, NETIS, civil engineering, and CLT, and we will continue to enhance the Q&A section.

Oct 11, 2026

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Hyper CLT flooring panel

Added a glossary to the website to make technical terms easier to understand.

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  • PRODUCT

We have added a new **"Glossary"** to the Hyper CLT sheet website. In the civil engineering and construction industries, there are many specialized terms such as "CLT," "NETIS," "temporary roads," and "wooden lightweight sheets." Therefore, we have introduced related terms in an easy-to-understand manner so that those who are encountering Hyper CLT sheets for the first time can understand the products and technologies. Hyper CLT sheets are wooden lightweight sheets for civil engineering that utilize wood CLT and are registered under the Ministry of Land, Infrastructure, Transport and Tourism's NETIS technology as **"TH-250021-A."** We hope that by not only conveying the features of the product but also sharing the underlying technologies and terminology, users will feel a closer connection to Hyper CLT sheets. We will continue to enhance our website and provide clear information related to wood, NETIS, civil engineering, and CLT.

Oct 10, 2026

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CCS3 product photo

The new product "Vehicle Count Sensor CCS3" enables efficient occupancy management of large parking lots.

  • NEW
  • PRODUCT

Hotron Inc. has launched its new product, the "Vehicle Count Sensor CCS3"! This product is a ceiling-mounted sensor that combines infrared and radar technology. It reliably detects vehicles in both directions with a single unit, accommodating traffic during congestion and vehicle passing, thus achieving efficient occupancy management in large parking lots. Additionally, it can be utilized for exit warning purposes in addition to vehicle counting, contributing to the safe and smooth operation of parking facilities. 'Features' - Improved detection performance against changes in road surface during rainy weather by using both infrared and radar. - Equipped with IP67 waterproof and dustproof performance, making it effective even in salt damage areas. - Various settings can be configured from a dedicated app (for Android devices), with current settings easily understandable at a glance. - The base dimensions are the same as the previous model "CCS2," allowing for smooth replacement of existing equipment. *For more details, please refer to the product information, catalog download, or contact us.*

Oct 09, 2026

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Hyper CLT flooring panel

On September 15, we issued a press release for the hyper CLT flooring.

  • NEW
  • PRODUCT

On September 15, 2026, we issued a press release regarding the wooden lightweight sheet "Hyper CLT Sheet." The Hyper CLT Sheet is a civil engineering sheet that utilizes CLT (Cross-Laminated Timber) stacked in an orthogonal direction. It can be used in various locations such as temporary roads, access routes, heavy machinery work yards, and material storage areas. The 5×10 version weighs approximately 230 kg, making it relatively lightweight and easy to install, remove, and relocate. Additionally, it offers environmental benefits such as reducing glare and metallic sounds typical of wood, as well as CO2 fixation. Furthermore, it can be proposed for the use of new technologies in public works as a **registered technology with the Ministry of Land, Infrastructure, Transport and Tourism "TH-250021-A."** Through this press release, we aim to expand the new wooden lightweight sheet that combines wood, NETIS, civil engineering, and CLT to more construction sites.

Oct 09, 2026

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Notice of the issuance of ISO-related information magazine "Intertek News" Issue 94.

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We have published the latest issue (No. 94) of the ISO-related information magazine "Intertek News." Intertek News delivers information and various topics related to ISO audits, certifications, and verifications. This issue features a special section titled "Interpreting Standard Revisions," which introduces key points of management system standard requirements that new personnel should understand as preparation for standard revisions. Additionally, we have included customer case studies, articles contributed by auditors, and information on certifications and standards, as well as the latest topics, so please take a look.

Oct 09, 2026

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