Highly sensitive detection of glutathione! It is also possible to evaluate the oxidized (GSSH) / reduced (GSH) ratio!
The "Glutathione Measurement Kit" is a kit for measuring total glutathione (oxidized + reduced) in samples derived from animals and plants. Glutathione in the sample reacts with DTNB (5-5'-dithiobis[2-nitrobenzoic acid]) to form TNB (5-thiobenzoic acid) and GS-TNB, which have an absorption at 412 nm. GS-TNB is broken down into TNB and GSH through a cyclic reaction by glutathione reductase, and this GSH reacts again with DTNB to produce TNB. By measuring the change in absorbance at 412 nm (or 405 nm), glutathione can be detected with high sensitivity. Additionally, this kit not only measures total glutathione but also allows for the evaluation of the oxidized (GSSG) / reduced (GSH) ratio by pre-treating the sample with 4-vinylpyridine to detect only oxidized glutathione. *For more details, please refer to the PDF document or feel free to contact us.*
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【Specifications】 ■Measurement targets: Whole blood, plasma, tissue homogenates, cultured cells, etc. ■Measurement range: 0.1–20 μmol/L ■Time required: Approximately 15 minutes ■Sample volume required: 50 μL ■Number of tests: 96 wells *For 2 repetitions (90 samples when N=2) ■Required equipment: - A microplate reader capable of measuring changes at 405 nm (able to measure for 3 minutes at 15–20 second intervals) - Micropipettes (25/50/100/500 μL) - Multi-channel pipette - 25–50 mL beaker - Distilled water - Eppendorf tubes or centrifuge tubes - 96-well flat-bottom microplate - Metaphosphoric acid (SIGMA 239275 or equivalent, only if performing protein removal from samples) ■Storage conditions: Refrigerated (2–10°C; do not freeze) *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Nikken Zail Co., Ltd. is a company that provides research reagents in a wide range of fields, including medicine, pharmacy, biochemistry, and food. Among them, the Japan Institute of Aging Control develops, manufactures, and sells research reagents in areas such as anti-aging, and also conducts contract testing. We will continue to enhance our research and development and production technology, while providing new products and services to contribute to the health of people around the world.
