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  6. Ambr15 Cell Culture System Generation 2

Ambr15 Cell Culture System Generation 2

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last updated:Nov 06, 2025

Sartorius Stedim Japan K.K.
Sartorius Stedim Japan K.K.
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Bioreactor system for cell line development and process optimization.

The Ambr1515 Generation 2 is a high-throughput "automated micro-scale bioreactor system" that reproduces the performance of laboratory-scale bioreactors. The system consists of single-use vessels, an automated workstation, and enhanced convenience software. It is designed to be installed in a standard biosafety cabinet for aseptic operations, allowing for parallel monitoring and control of 24 or 48 micro-bioreactor cultures. As the manufacturing processes for biopharmaceuticals become more complex and the density of cultures increases, scientists around the world are facing an unprecedented challenge in developing "clone, medium, and process" that meet new demands. The Ambr15 Generation 2 system enhances equipment performance that influences the success of cultures, improves flexibility, and incorporates new features that reflect user feedback. It helps address the aforementioned challenges and supports the acceleration of research by scientists.

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Ambr-15CC-Gen2-operator.jpg

Ambr15 Cell Culture System Generation 2

Ambr-15CC-Gen2-operator.jpg
Ambr-15CC-Gen2-operator.jpg ambr-15CC_overview.JPG ambr15_24&48.JPG ambr15_Gen2_w_operator_10046.jpg
  • Ambr15 Cell Culture Generation 2
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■ The new features improve the performance of applications such as the following: - Clone selection - Optimization of culture media and feeds - High-density culture processes - Media exchange and passaging in cell therapy applications ■ The new variable deck reduces operator downtime and improves operator efficiency ■ The new Amber clone selection software makes the best clone decision process easier ■ The new Generation 2 liquid handler enables faster and more precise operations, capable of complex tasks such as liquid mixing and addition in multifactorial design of experiments (DoE) ■ Ambr15 is the industry-standard micro-bioreactor system used in research facilities worldwide ■ The addition of metabolite analysis equipment enables a development approach in accordance with Quality by Design (QbD) ■ Ambr15 significantly reduces the amount of culture media and consumables, thereby lowering the cost per experiment, in addition to saving on facility space, funding, and labor ■ Ambr15 contributes to better clone selection on-site, more media selection, and faster process optimization

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Applications/Examples of results

Ambr15 is used as a micro-scale model that resolves various bottlenecks in applications such as the following: - Clone selection - Optimization of media, feeds, and initial processes - Screening under perfusion-like conditions Ambr15 is an industry-standard micro-bioreactor system widely used in the development of biopharmaceuticals such as monoclonal antibodies and recombinant proteins. Ambr15 is increasingly being used to accelerate the development of newer methods such as cell therapy, gene therapy, bispecific antibodies, next-generation vaccines, and antibody-drug conjugates (ADCs).

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Ambr15 Cell Culture System Generation 2 Catalog

Ambr15 Cell Culture System Generation 2 Catalog

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StreamLink CC 15

StreamLink CC 15

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Ambr 15 Generation 2 options, accessories, and consumables

Ambr 15 Generation 2 options, accessories, and consumables

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CHO Cell Culture Optimization: A DOE Approach in the Ambr 15

CHO Cell Culture Optimization: A DOE Approach in the Ambr 15

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Custom-made culture medium service utilizing specialized knowledge.

Custom-made culture medium service utilizing specialized knowledge.

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Resource Roadmap for Successful Screening: Cell Therapy Edition

Resource Roadmap for Successful Screening: Cell Therapy Edition

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[Free Domestic Webinar] Perfusion Culture Solutions Provided by Sartorius

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This webinar will focus on perfusion culture, which is gaining attention for process enhancement, and will introduce examples of utilizing Sartorius solutions. The bioreactors Ambr 15, Ambr 250 HT, and Biostat STR are compatible with perfusion culture. This time, we will explain the mechanisms and operational methods using Ambr 15 and Ambr 250 HT. Additionally, our CHO cell line development service utilizes the 4Cell SmartCHO medium, which can accommodate both fed-batch and perfusion cultures, and can also be used for suitable clone selection. Through examples that combine Ambr 15 and Ambr 250 HT with the same medium, we will convey the image of its application in process development. 【Seminar Overview】 ▪ Date: July 16 (Thursday) from 15:00 (approximately 30 minutes) ▪ Format: Webinar recorded broadcast [Japanese, Free] If you are unable to participate on the day, please register first. We will send you a URL for viewing later. *We kindly ask that participants refrain from registering with email addresses other than those from your own company or organization. Thank you for your understanding.

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[Free Domestic Webinar] Introduction to the Industry Standard Bio-Reactor Ambr250 High Throughput Generation 2 Optimized for Process Characterization Analysis

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The Ambr 250 High Throughput is a high-throughput bioreactor that accelerates process development and process characterization analysis. It enables simultaneous cultivation of up to 24 samples while incorporating automated control via a robotic arm and integration with external analytical devices. The culture containers are single-use specifications of 100-250 mL and are compatible with perfusion culture and microcarrier culture. This device has been enhanced based on customer feedback and was launched as the second generation, Ambr 250 High Throughput Generation 2, starting in 2025 to demonstrate further performance as a scale-down model. In this webinar, we will introduce the device overview and the enhanced features in Generation 2. 【Seminar Overview】 ▪ Date: October 16 (Thursday) at 15:00 (approximately 30 minutes) ▪ Method: Webinar recorded broadcast [Japanese, Free] *We apologize, but participation from companies in the same industry and email addresses other than those of your organization is not permitted. Thank you for your understanding.

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Domestic Webinar: CHO Media and Process Development Solutions to Support Optimal Media Selection for Your Cell Line

  • Seminar・Event

To maximize the performance of CHO cells, it is necessary to select the optimal medium for each clone and further optimize the process parameters. Sartorius offers a variety of basic media and feeds suitable for Fed-batch culture of major CHO cell lines such as CHO-DG44, K1, S, and GS. In this webinar, we will introduce a case study that combines these CHO media, the automated microbioreactor Ambr15, and the experimental design software MODDE to conduct medium screening and process optimization in a short period. We will also provide information about our Benchmark Study service, which allows you to outsource the selection of the optimal medium and process development for your specific cell line to our research institute in Germany. 【Seminar Overview】 ▪ Date: March 6, 2025 (Thursday) from 3:00 PM (approximately 40 minutes) ▪ Method: Webinar recorded broadcast [Japanese, Free] If you are unable to participate on the day, please register first. We will send you a viewing URL later. *We kindly ask that participants from other companies in the same industry, as well as those using email addresses not affiliated with your organization, refrain from joining. Thank you for your understanding.

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[End] Innovation Forum 2023 "Simplification of pDNA|mRNA Manufacturing Process by Sartorius"

  • Seminar・Event

This event has concluded. *Please note that there are currently no plans for on-demand distribution. mRNA pharmaceuticals are attracting attention as a new modality for drug discovery. Sartorius has a wide range of technologies that cover the mRNA manufacturing process, including E. coli culture, pDNA purification, IVT reactions, mRNA purification, and freeze storage. In this forum, we will provide a meaningful discussion platform centered around topics such as "pDNA|mRNA Manufacturing Processes," "Sartorius Manufacturing Platforms," and "Application Examples," inviting guest speakers to give presentations and facilitating questions and interactions from participants. Special Lecture Kazuhisa Uchida, Graduate School, Kobe University Capacity [In-person participation] 35 people *If there are many applications, a lottery will be held. [Online participation] 200 people *Please note that applications from competitors and research companies may be declined in advance.

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Sartorius

Sartorius Stedim Japan K.K.

Pharmaceuticals and Biotechnology

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Sartorius Stedim, established in 2007 through the merger of the German company Sartorius, founded in 1870, and the French company Stedim, is a pioneer in "Single-Use Technology (SUT)." As a company capable of providing comprehensive technical support to the pharmaceutical and biotechnology industries, its cutting-edge technology is highly regarded worldwide in the fields of scientific instruments and biotechnology.

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