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  4. Protein Research Reagents
  5. Research Antibodies
  6. Differences in amino acids: Antibodies that can distinguish slight differences.

Differences in amino acids: Antibodies that can distinguish slight differences.

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last updated:Oct 03, 2022

ジーンフロンティア
ジーンフロンティア
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Currently offering free consultations + advice available. What if you could distinguish one amino acid using convenient tools like antibodies? Here are examples of creating highly specific antibodies.

With "Antibody Craftsman," selection is performed in vitro against fixed antigens without using animal immunization. This allows for binding reactions to be conducted in the state or environment of prepared peptides or proteins, enabling detailed antigen design, such as narrowing down specific areas. This document is a must-read for those who want to learn more about the selection of antibodies that can distinguish a single amino acid substitution, so please feel free to take a look. [Contents (excerpt)] - Preparation of antigens - Selection to increase specificity - Selection of antibodies *For more details, please refer to the PDF document or feel free to contact us.

    Research Antibodies
スクリーンショット

Differences in amino acids: Antibodies that can distinguish slight differences.

スクリーンショット 2022-10-03 152830.jpg
スクリーンショット 2022-10-03 152830.jpg

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basic information

▼First, Planning▼ The "Antibody Craftsman" monoclonal antibody production service begins with planning when we receive inquiries from customers about antibody production. To create antibodies suitable for the customer's intended use, we will ask about the characteristics of the antigen and the purpose of the antibody. Next, we will design the antigen according to the purpose of the antibody to be obtained and propose strategic selection methods tailored to the type of antigen and the intended use of the antibody, guided by experienced personnel throughout the entire process. ▼Format and Reproduction of Delivered Antibodies▼ The antibodies produced will be in the form of Fab, with options for monovalent or divalent selection and two types of tags, delivered in the desired format. The obtained antibodies will be purified as recombinant proteins from E. coli, allowing us to deliver them with the same quality repeatedly. Additionally, converting Fab to immunoglobulin isotypes such as IgA, IgE, IgG, and IgM can be easily accomplished.

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※At the shortest, 7 weeks from the receipt of the antigen.

Applications/Examples of results

Antibodies produced by "Antibody Craftsman" can be used in various applications just like antibodies produced by conventional methods. Furthermore, since they are Fab antibodies, they possess properties that can provide additional advantages in certain assay systems, such as not needing to remove the Fc region to prevent binding to receptors or to minimize the reaction of human anti-mouse antibodies (HAMA). Additionally, tag-specific detection antibodies are very useful in many applications.

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Custom Monoclonal Antibody Production Contract Service "Antibody Craftsman"

Custom Monoclonal Antibody Production Contract Service "Antibody Craftsman"

TECHNICAL
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[Antibody Craftsman Case] Selection of antibodies that distinguish a single amino acid substitution.

[Antibody Craftsman Case] Selection of antibodies that distinguish a single amino acid substitution.

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ジーンフロンティア

ジーンフロンティア

Chemical

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Gene Frontier has been expanding its business areas that contribute to the acceleration of biopharmaceuticals, including antibody drugs, and has been advancing the development of its unique technologies. Moving forward, we aim to accelerate our research and development and business development to achieve further growth on a global scale. We will continue to provide unique technologies and services for the development of innovative pharmaceuticals and diagnostic drugs, striving to be a trusted partner for our customers and contribute to 21st-century healthcare.

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