Enzyme 'Bst DNA Polymerase' for isothermal gene amplification technology LAMP method

Bst DNA polymerase is an enzyme that can be used in the LAMP method. The LAMP method is a technique for amplifying and detecting target DNA or RNA in a short period of time.

This product is an enzyme that has 5'→3' DNA polymerase activity and strand displacement activity, capable of synthesizing new DNA strands while dissociating the hydrogen bonds of the double-stranded DNA template. The heat-stable strand-displacing DNA polymerase does not require the dissociation of double-stranded DNA due to its characteristics, allowing DNA synthesis at a constant temperature, and it is not affected by synthesis inhibition caused by the secondary structure of DNA. 【Features】 ■ Has 5'→3' DNA polymerase activity and strand displacement activity ■ Capable of DNA synthesis at a constant temperature ■ Suitable for synthesizing DNA strands with high GC content ■ Not inhibited by the secondary structure of DNA 【Optimal Reaction Temperature】 60°C to 65°C 【Inactivation Time】*1 80°C for 5 minutes *1 Inactivation temperature when the enzyme stock solution is directly heat-denatured 【Applications】 Applications utilizing strand displacement activity (such as isothermal gene amplification methods)

Other Amplification/PCR