This paper presents examples of measuring non-fixed cells under physiological conditions to visualize the distribution and localization of cellular components (such as proteins and nucleic acids).
The technical information magazine The TRC News provides the latest information on analytical techniques that are useful for research and development, solving production troubles, and quality control. **Abstract** Infrared spectroscopy is a method for obtaining information about molecular structure by measuring infrared absorption due to molecular vibrations. Utilizing photothermal conversion, O-PTIR (Optical Photothermal IR Spectroscopy) enables infrared analysis with sub-micron spatial resolution and non-contact measurement. This paper presents a case study of measuring non-fixed cells under physiological conditions to visualize the distribution and localization of cellular components (such as proteins and nucleic acids). **Table of Contents** 1. Introduction 2. Overview and Features of O-PTIR 3. Analysis of Suspended Living Cells (Budding Yeast) in Water - Comparison of Intracellular Spectra - 4. Analysis of Suspended Living Cells (Budding Yeast) in Water - Distribution of Cellular Components - 5. Conclusion
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Technical Information Magazine The TRC News "Analysis of Suspended Living Cells in Water Using Optical-PTIR" [Abstract] Infrared spectroscopy is a method for obtaining information about molecular structure by measuring infrared absorption due to molecular vibrations. O-PTIR (Optical Photothermal IR Spectroscopy), which utilizes photothermal conversion, enables infrared analysis with sub-micron spatial resolution and non-contact measurement. This paper presents a case study of measuring non-fixed cells under physiological conditions to visualize the distribution and localization of cellular components (such as proteins and nucleic acids). [Table of Contents] 1. Introduction 2. Overview and Features of O-PTIR 3. Analysis of Suspended Living Cells in Water (Budding Yeast) - Comparison of Spectra within Cells - 4. Analysis of Suspended Living Cells in Water (Budding Yeast) - Distribution of Cellular Components - 5. Conclusion [Figures and Tables] Figures 1-8 Challenges of FT-IR Measurement Practical Measurement of Yeast Cells Observation Results and Measurement Sites of Yeast Cell Suspensions Comparison of Spectra Near the Cell Wall A) and Center B) of Yeast Cells Distribution of Proteins A) and Nucleic Acid Components B) in Yeast Cells Distribution of Nucleic Acid Components in Yeast Cells
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At Toray Research Center, Inc., we provide technical support through analytical techniques and physical property analysis to address various challenges in research and development as well as production fields via contract analysis. Based on our long-standing achievements and extensive experience in analysis and physical property evaluation, we continue to strive to meet the advanced and diverse needs of our customers.
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