Evaluation method for suppressing biofilm formation on substrates

Material development is underway to suppress the formation of biofilms!

We would like to introduce our "Biofilm Formation Suppression Evaluation Method on Substrates," which is part of our biofilm-related analysis. To evaluate the suppression of biofilm formation, the substrate is first immersed in a bacterial culture solution to allow biofilm formation. The formed biofilm is then stained with crystal violet (CV), after which the CV is extracted with an extraction solution, and the absorbance is measured. This is compared to the biofilm formation amount on a standard substrate. 【Analysis Method】 1. Immerse the substrate in a bacterial culture solution and incubate at a constant temperature for 24 to 48 hours. 2. Rinse the substrate with water and stain the formed biofilm with crystal violet. 3. Extract the crystal violet and measure the absorbance at 590 nm. 4. Compare with the biofilm formation amount on the standard substrate. *For more details, please refer to the PDF document or feel free to contact us.

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