Ex vivo evaluation test using microdialysis method.

Analysis of the mechanism of action of skin permeability and the effects of components on the skin.

■Recommended for those with the following development challenges: - Want to elucidate the mechanisms of action of cosmetic and pharmaceutical ingredients on the skin. - Want to evaluate the skin permeability of cosmetic and pharmaceutical ingredients and quantify their metabolites. - Want to quantify the temporal changes of IL-8 after drug administration. - Want to conduct experiments under conditions that impose difficult stimuli or burdens on humans. ■Evaluation Features: - By using extremely fresh excised skin, it is possible to analyze the effects of test substances on the skin and their mechanisms in a state almost identical to human skin (such as stratum corneum barrier function). - In addition to measuring the amount of permeation of ingredients, it is also possible to quantify substances generated in the skin, such as metabolites and inflammatory factors. - It is possible to apply electrical or light stimuli, as well as create conditions of irritated skin, which are difficult to implement on humans. - Ideal for evaluating the usefulness of raw materials and formulations of cosmetics and pharmaceuticals, as well as physical stimulation from beauty devices. ■Test Overview: Extremely fresh human excised skin is used within a few hours after excision. A semi-permeable membrane is attached to the tip of a microprobe through which perfusion fluid circulates, and it is inserted into the dermis. The dialysis fluid after the use of the test substance is collected and quantified.

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