Industry-first high-throughput gel filtration PhyTip columns for desalting and buffer exchange at small scale and multiple samples.
The chip-type gel filtration PhyTip column filled with size exclusion chromatography resin is optimal for desalting protein samples and exchanging buffer, and it also enables high-throughput screening (HTS) in a 96-well plate. Desalting using the gel filtration PhyTip column can be set up as a secondary purification step after affinity purification with an affinity PhyTip column, such as the removal of imidazole after IMAC affinity purification, using the same automated protocol. Like other PhyTip columns, the resin is packed between two hydrophilic screens; however, unlike other PhyTip columns, the gel filtration column passes the sample from the top. The gel filtration PhyTip column has size exclusion resins of 5K and 30K, which retain low molecular weight compounds while allowing molecules of 5 kDa and 30 kDa or larger to pass through. Unlike other PhyTip columns, the use of the ME system is not required. Major liquid handling robots can be used for multi-sample processing. *For more details, please visit our website or feel free to contact us.*
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basic information
- Removal of over 95% of salt, recovery rate of over 80% for proteins - Capable of automation for high-throughput - Free fall from top to bottom (one-directional gel filtration, not Dual Flow Chromatography technology) - Can separate without compromising the function of antibodies and proteins - High reproducibility (low variability between samples; CV below 10%) *For more details, please visit our website or feel free to contact us.*
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Applications/Examples of results
This is an optimal gel filtration purification for small amounts and multiple samples. In addition to manual operation, a liquid handler robot equipped with a gripper arm can also be used in a 96-well format, enabling high-throughput screening (HTS) with gel filtration.
Detailed information
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The gel filtration PhyTip does not incorporate the unique technology of Dual Flow Chromatography characteristic of the PhyTip column, and instead, it purifies the sample by allowing it to flow in one direction due to gravity.
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Use of gel filtration PhyTip columns with liquid handler robot gripper arms Main liquid handlers compatible with gel filtration PhyTip columns: - Agilent Technology - Beckman Coulter - Dynamic Devices - Hamilton - Opentrons - Perkin Elmer - Tecan
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Company information
Biotage Japan Co., Ltd. is a wholly-owned subsidiary of Biotage AB, which is headquartered in Uppsala, Sweden (BIOT: NASDAQ/OMX Stockholm). Biotage AB has its roots in several venture companies that originated from Uppsala University, a leading science and engineering university in the Nordic region. After undergoing international acquisitions and restructuring throughout the 2000s, it has recently achieved remarkable growth as a global company.
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