Tohoku Univ. Technology : Aspergillus oryzae introduced with multi-copy genes : T24-084

Extremely high production level of target protein by fermentation (3 g/L)

　In protein production using Aspergillus oryzae, the introduction of multi-copy genes encoding target proteins can be used as a means of improving productivity. However, if the DNA fragment to be introduced is tandemly inserted at one chromosomal locus and the repair through homologous recombination is repeated, the sequence may be excised, and the production cannot be increased as a result. 　Dr. Zhang and colleagues at the Graduate School of Agriculture, Tohoku University have developed a method to introduce DNA fragments into multiple loci of the chromosome of A. oryzae in a single transformation, and succeeded in obtaining a new A. oryzae in a short period of time. The A. oryzae of the present invention has been confirmed to have improved material productivity. Furthermore, by using a highly dispersible strain of mycelium as a host, it is expected to be low in viscosity when cultured in a fermenter, enabling continuous culture.

others