A new biotin labeling agent that allows control over the introduction location and quantity [Saitama University]

The key is the re-crosslinking of S-S bonds! A novel biotin labeling agent that stabilizes the structure and function of proteins.

Conventional biotin labeling randomly binds to the functional groups of proteins, making it difficult to control the number of biotins attached and their binding positions. Additionally, the cleaved S-S bonds for labeling do not re-bridge, leading to changes in the higher-order structure of the protein and a decrease in activity, which is a challenge. In this technology, biotin is introduced to the Br group of dibromo-pyridazine dione, and the re-bridging of S-S bonds occurs simultaneously with labeling. This stabilizes the higher-order structure of the protein and maintains its activity. The number of attached biotins can be selected as either one or two, and it can be used for controlling the orientation of proteins by utilizing the binding characteristics with streptavidin.

Chemicals