Advantages of 3'-acetoxime-2'-deoxy nucleosides!
This novel nucleoside building block offers excellent advantages compared to conventional reagents with protective groups, as outlined below. 1. Extremely mild deprotection conditions - Conventional deprotection: In standard oligonucleotide synthesis, harsh conditions such as treatment with concentrated ammonia or high temperatures were required to cleave the oligomer from the support and remove protective groups (especially acyl protecting groups like benzoyl or isobutyryl). These conditions could damage unstable sequences, such as modified nucleosides or long-chain oligonucleotides. - Acetoxime protecting group: The 3'-acetoxime protecting group can be rapidly removed under extremely mild conditions, such as treatment at room temperature with water or weakly basic buffers (e.g., triethylammonium carbonate at pH 7-8). This dramatically improves purity and yield in the synthesis of complex probes and drugs, particularly those containing molecules sensitive to heat or bases (e.g., fluorescent dyes, thiolate modifications). 2. Reduction of synthesis cycle time Mild deprotection conditions shorten the overall time of the synthesis process.
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Applications/Examples of results
This innovative nucleoside building block is ideal for cutting-edge nucleic acid technologies such as the following: * Synthesis of unstable modified oligonucleotides It is optimal for the synthesis of molecules used as nucleic acid drugs, such as siRNA (small interfering RNA), antisense oligonucleotides (ASO), or aptamers. These molecules often incorporate ligands that improve cellular uptake and chemical modifications that enhance stability, making gentle synthetic pathways essential. * Development of nucleic acid probes and diagnostics High-sensitivity fluorescent dyes included in FISH (fluorescence in situ hybridization) probes and qPCR probes may degrade or become inactive under harsh conditions. The acetoxime method preserves the integrity of the dyes and enables the production of high-precision diagnostic agents. * Optimization of automated synthesis It can be easily integrated into automated synthesis protocols using solid-phase synthesis machines, achieving reliable and reproducible oligonucleotide synthesis.
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Ayurveda is well-known, but historically, India has been significantly involved with "medicine." Sapala Organics was established in 2005 in Hyderabad, located in the central part of the Deccan Plateau. The state government is actively investing to make this area a "Pharma City" (a treasure trove of medicine). Sapala has gained recognition from major pharmaceutical companies in the United States and Europe, steadily achieving results. To further provide the technological capabilities developed globally to Japan, Sapala Corporation was established in February 2018 as a local subsidiary in Japan. Recently, the Japanese government has also been focusing on projects that promote advanced pharmaceutical development and drug discovery, including nucleic acid-related drugs. We encourage you to consider the technological expertise of "Sapala," which excels in these fields.
