サパラ Product Lineup

Introduction to High Purity Morpholino Chlorophosphoramidite As gene therapy and antisense drug development open up new possibilities in medicine, the key to their success lies in the high-performance artificial nucleic acid known as Morpholino Oligomer (PMO). Sapala Organics combines years of expertise in nucleic acid chemistry with cutting-edge technology to provide high-quality Morpholino synthesis reagents that strongly support your next-generation oligonucleotide drug development. What is Morpholino? Morpholino Oligomer (PMO) is a type of artificial nucleic acid that replaces the sugar (ribose) backbone of natural nucleic acids with a Morpholino ring, and the ionic phosphodiester bond with a non-ionic phosphorodiamidate bond.

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★The Main Features and Utility of L-DNA The "L" in L-DNA indicates that the stereochemistry of the sugar component (deoxyribose) is the mirror image isomer (enantiomer) of the naturally occurring D-DNA (D-deoxyribose). This mirror relationship is the source of L-DNA's utility. 1. Stability in Biological Systems (Nuclease Resistance) The greatest advantage is its extremely high resistance to nucleases (nucleic acid degrading enzymes). Enzymes present in the human body have evolved to recognize and degrade D-DNA. Because the enzymes themselves are chiral (asymmetric), they cannot recognize or bind to L-DNA, which is a mirror image isomer. 2. Non-Hybridization with D-DNA/RNA L-DNA does not form Watson-Crick base pairs with D-DNA or D-RNA, nor does it coil into a double helix. However, L-DNA can form a stable double helix (left-handed Z-type structure) with other L-DNA molecules. This property allows for the avoidance of unintended hybridization (binding) with genomic DNA or mRNA in biological systems, which is expected to reduce noise in assay systems and suppress off-target effects.

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The catalog shows four types of GNA units with protective groups that are essential for oligonucleotide synthesis. • DMT-GNA-A(Bz) (CAS: 115196-70-8) • DMT-GNA-G(iBu) (CAS: 182625-67-8) • DMT-GNA-T (CAS: 168332-12-5) • DMT-GNA-C(Bz) (CAS: 127757-40-8) These have a 5' terminal equivalent OH group protected by a DMTr (dimethoxytrityl) group, and the bases (A, G, C) are also protected by standard Bz (benzoyl) or iBu (isobutyl) groups.

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Provided lineup (excerpt) The catalog includes the main LNA amidites: LNA-A(Bz) amidite (CAS: 206055-79-0) LNA-G(dmf) amidite (CAS: 709641-79-2) LNA-T amidite (CAS: 206055-75-6) LNA-C(Bz) amidite (CAS: 206055-82-5) Characteristics of LNA phosphoramidites The most notable feature of LNA is that the 2'-O and 4'-C of the ribose ring are linked (locked) by a methylene bridge. The advantages brought by this structure are remarkable. 1. Advantages of LNA ■ Improved thermal stability (increased T_m value) ■ Enhanced nuclease resistance ■ Excellent mismatch discrimination ability 2. Disadvantages to be aware of and "smart" usage ■ Off-target effects and toxicity risks ■ Lack of flexibility

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FANA phosphoramidite reagents from Sapala Organics The FANA (2'-fluoro-arabinonucleic acid) phosphoramidites listed in this catalog are as follows: (1) FANA-A(Bz) amidite CAS 2882846-29-7 (2) FANA-G(iBu) amidite CAS 1404463-20-2 (3) FANA-C(Bz) amidite CAS 159414-99-0 (4) FANA-T amidite CAS 208193-48-0

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This catalog demonstrates the applications and advantages of Dimer & Trimer Capping reagents listed herein. Dimer and Trimer Caps are utilized as building blocks in the block coupling method for oligonucleotide (nucleic acid) synthesis, particularly in RNA synthesis, providing several important benefits.

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In the development of oligonucleotide drugs, precise structural control of the backbone will be the key to the next generation. Sapala Organics proposes the "C5'-extended 2'-substituted phosphoramidite" to overcome this challenge. Features of C5'-extended 2'-substituted phosphoramidite: The most significant feature of this product is that it physically extends the "distance" between nucleotides by elongating the C5' position. This allows for the resolution of steric hindrance that was difficult to avoid with conventional nucleic acid structures and enables optimal orientation control towards target molecules. Furthermore, a diverse range of modifications such as H, OMe, MOE, and F are available at the 2' position, supporting the advanced coexistence of nuclease resistance and binding affinity.

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"Three Innovations Brought by 4'-Thio Modification" 1. Excellent Nuclease Resistance (Approximately 600 Times Higher than Natural) 4'-thioRNA has a structure in which the oxygen at the 4' position of the ribose ring is replaced by sulfur. This slight change creates stability. 2. Strong and Accurate Hybridization Ability In addition to stability, the binding strength to the target (Tm) is also enhanced, which is a strength of 4'-thio. - Increase in Tm: An increase of approximately 1°C in Tm is expected per modification, providing a stabilization effect comparable to that of 2'-O-methyl modifications. - Maintenance of A-type Structure: By inducing the sugar moiety into an RNA-like "C3'-endo conformation," it improves thermal stability while maintaining a structure close to natural RNA. 3. Compatible with Existing Platforms Despite being a new technology, its low introduction cost is a significant advantage. - Standard Synthesis Method: Supplied in DMTr-protected and phosphoramidite form, it can be used directly with your existing automated synthesizers. - Smooth Transition: Base protection uses standard methods (such as Bz, iBu), allowing the deprotection and purification processes to be optimized based on existing protocols.

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We are pleased to introduce a groundbreaking building block series, "3'-Azidomethyl-2'-deoxy-nucleosides" (dA, dG, dC, dU), that will accelerate your company's next-generation nucleic acid drug development program. The key feature of this strategic material is the unique azidomethyl group at the 3' position of deoxyribose. This structure provides a powerful advantage that is not found in conventional modified nucleosides. - Utilization and Three Powerful Advantages Here are three powerful advantages gained by utilizing this reagent: 1) High-efficiency functionalization (click chemistry) 2) Improved stability and persistence 3) Chain terminator

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This novel nucleoside building block offers excellent advantages compared to conventional reagents with protective groups, as outlined below. 1. Extremely mild deprotection conditions - Conventional deprotection: In standard oligonucleotide synthesis, harsh conditions such as treatment with concentrated ammonia or high temperatures were required to cleave the oligomer from the support and remove protective groups (especially acyl protecting groups like benzoyl or isobutyryl). These conditions could damage unstable sequences, such as modified nucleosides or long-chain oligonucleotides. - Acetoxime protecting group: The 3'-acetoxime protecting group can be rapidly removed under extremely mild conditions, such as treatment at room temperature with water or weakly basic buffers (e.g., triethylammonium carbonate at pH 7-8). This dramatically improves purity and yield in the synthesis of complex probes and drugs, particularly those containing molecules sensitive to heat or bases (e.g., fluorescent dyes, thiolate modifications). 2. Reduction of synthesis cycle time Mild deprotection conditions shorten the overall time of the synthesis process.

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Sapara Corporation is a company based in Hyderabad, India, that conducts contract research, contract manufacturing, and contract analysis of organic substances. With a research system equipped with various instruments and facilities, we ensure thorough quality control, and our creative and innovative technical team responds sincerely. Additionally, Sapara Corporation's business areas cover a wide range, including nucleic acid-related compounds, fluorescent dyes, and carbohydrate chemistry. 【Features】 ■ Trusted worldwide for "contract research, contract manufacturing, and contract analysis" achievements ■ A research system equipped with various instruments and facilities ■ Thorough quality control and sincere responses from a creative and innovative technical team ■ Strict adherence to deadlines based on planned and rigorous schedule management ■ Quick responses through agile and dynamic activities *For more details, please refer to the PDF materials or feel free to contact us.

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Sapara Corporation is a company based in Hyderabad, India, that conducts contract research, contract manufacturing, and contract analysis of organic substances. The region of Hyderabad is very famous for IT-related activities, but it is also a vibrant area for research and development in the field of pharmaceuticals. Sapara Corporation has strengths in research and development of specialized organic compounds, process design, and analysis, and has received patronage from major countries around the world, including not only Japan but also Europe and the United States. Recently, we have achieved notable results in the field of research and development related to nucleic acid pharmaceuticals. Additionally, our founder has over 20 years of work experience in Japan and is fluent in the language, so we are confident that we can deliver results that will satisfy our Japanese clients. By the way, the name "Sapara" is derived from the initials of the three goddesses of India. *You can download the English version of our catalog. *For more details, please refer to the PDF materials or feel free to contact us.

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At Sapara, many chemists with advanced technical skills are actively engaged in the synthesis of complex organic compounds used in life sciences and functional materials, earning the trust of customers worldwide. In our research facilities equipped with cutting-edge experimental and analytical instruments, over 190 outstanding chemists are dedicated to research day and night. Our extensive experience in various stages of oligonucleotide processes, including structural design, synthesis, and scale-up, which are actually used around the world, serves as the driving force behind the trust we receive from our customers. 【Features】 ■ Contract Research | Contract Manufacturing | Contract Analysis ■ Certifications: ISO 9001:2015, ISO 27001:2013 ■ Contributing to the acceleration of research and development *For more details, please refer to the PDF document or feel free to contact us.

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