Advantages and disadvantages of LNA
Provided lineup (excerpt) The catalog includes the main LNA amidites: LNA-A(Bz) amidite (CAS: 206055-79-0) LNA-G(dmf) amidite (CAS: 709641-79-2) LNA-T amidite (CAS: 206055-75-6) LNA-C(Bz) amidite (CAS: 206055-82-5) Characteristics of LNA phosphoramidites The most notable feature of LNA is that the 2'-O and 4'-C of the ribose ring are linked (locked) by a methylene bridge. The advantages brought by this structure are remarkable. 1. Advantages of LNA ■ Improved thermal stability (increased T_m value) ■ Enhanced nuclease resistance ■ Excellent mismatch discrimination ability 2. Disadvantages to be aware of and "smart" usage ■ Off-target effects and toxicity risks ■ Lack of flexibility
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Disadvantages and "Smart" Usage You Should Know LNA is not a magic wand that simply works by being introduced. There are points of caution due to its characteristics. ● Risks of Off-Target Effects and Toxicity - Issue: Due to its high affinity, it may bind to unintended mismatched sequences (off-target effects). Additionally, the extensive use of LNA with specific sequence patterns has been reported to potentially cause hepatotoxicity. - Countermeasure: Instead of making the entire sequence LNA, it is recommended to design it as a "Mixmer" mixed with DNA or a "Gapmer" with DNA placed in the center. ● Lack of Flexibility - Issue: The structure is fixed in the C3'-endo conformation, resulting in a loss of flexibility in the double strand. - Countermeasure: If enzyme reactions (such as degradation by RNase H) are expected, careful design of the placement of LNA is necessary.
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Ayurveda is well-known, but historically, India has been significantly involved with "medicine." Sapala Organics was established in 2005 in Hyderabad, located in the central part of the Deccan Plateau. The state government is actively investing to make this area a "Pharma City" (a treasure trove of medicine). Sapala has gained recognition from major pharmaceutical companies in the United States and Europe, steadily achieving results. To further provide the technological capabilities developed globally to Japan, Sapala Corporation was established in February 2018 as a local subsidiary in Japan. Recently, the Japanese government has also been focusing on projects that promote advanced pharmaceutical development and drug discovery, including nucleic acid-related drugs. We encourage you to consider the technological expertise of "Sapala," which excels in these fields.
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