Random Integration Analysis Service using the RAISING Method
Developed through joint research with the National Institute of Infectious Diseases. Achieves determination of the insertion site of foreign DNA in a short time, with high sensitivity and low cost.
The "RAISING method" is a technique for detecting the insertion sites of foreign DNA in the genome. It allows for rapid and reproducible measurement results with a small amount of DNA. It can identify insertion sites of viral genome insertions, viral vector insertions, searches for fusion genes that could be drug discovery targets, and off-target knock-ins in genome editing, among others. 【Features】 - Analysis can be arranged according to the purpose and target. - Multiple insertion sites can be detected in a single analysis. - High reproducibility of results is achieved through proprietary technology. 【Analysis Examples】 - HTLV-1 (Human T-cell Leukemia Virus) Clonality Analysis Leveraging the characteristics of the RAISING method, which can quickly detect with a small amount of DNA, we achieved high detection sensitivity even with a proviral load of 0.032%. - Off-target Detection of Knock-ins in Genome Editing For knock-in mice created using CRISPR/Cas, we conducted detection of knock-in efficiency and off-target sites by combining with NGS. *For details on examples and services, please refer to the materials available for download.
- Company：ファスマック
- Price：Other
![[Case Study] Determination of Insertion Sites for Animal Cultured Cells and Plant Cells](https://image.mono.ipros.com/public/product/image/601/2000622982/IPROS61554638147138618726.png?w=280&h=280)
