関東化学株式会社 List of Products and Services

The PCR-based ORF Typing (POT method) developed by Dr. Masahiro Suzuki at Fujita Medical University is a method for comparing the homology between bacterial strains by simultaneously amplifying multiple specific genes using multiplex PCR and analyzing the amplified band patterns. Features: - All necessary reagents are included in the PCR kit. - Molecular epidemiological analysis can be completed in about 4 hours. - Comparable analytical capability to PFGE, MLST, etc. - The analysis results are quantified (POT value), making it easy to compare and manage the homology between bacterial strains.

The "Shikajenius Bacterial-Related Gene Detection Kit Series" is a PCR kit that can detect drug resistance genes (β-lactamase) simultaneously using multiplex PCR. 【Features】 ● Detection is possible in about 3 hours with a standard thermal cycler. ● The PCR conditions are the same for all three kits. 【Target Genes for Detection】 Shikajenius Carbapenemase Gene Type Detection Kit 2 → Detection of gene groups for IMP-1, VIM, KPC, NDM, OXA-48, and carbapenemase type GES, and differentiation of the IMP-6 gene. Shikajenius ESBL Gene Type Detection Kit 2 → Detection of CTX-M-1, CTX-M-2, CTX-M-8, CTX-M-9, CTX-M-25, CTX-M chimera, ESBL type GES, TEM, and SHV gene groups. Shikajenius AmpC Gene Type Detection Kit → Detection of the CIT, FOX, ACT, DHA, ACC, and MOX gene families.

This product can simultaneously detect pathogenic genes related to five main types of enteropathogenic Escherichia coli (E. coli) using multiplex PCR: EHEC/VTEC (enterohemorrhagic/verotoxin-producing E. coli), EPEC (enteropathogenic E. coli), ETEC (enterotoxigenic E. coli), EIEC (enteroinvasive E. coli), EAggEC (enteroaggregative E. coli), and E. albertii. **Features** - Using two types of primer mixes, it can simultaneously and distinctly detect species-specific genes of E. coli and E. albertii along with seven types of pathogenic factors*1. (*1 Pathogenic factors: Verotoxin types 1 and 2 (VT1, 2) genes (stx1, 2), heat-stable enterotoxin (ST) gene (est), heat-labile enterotoxin (LT) gene (elt), intimin gene (eae), aggregative adherence factor (aggR), enteroinvasive factor (invE)) - By quantifying the detection results, the judgment results can be clarified. - Reagents necessary for PCR, including positive controls, are included. - The detection results of the internal control can confirm the presence or absence of PCR inhibition.

In this series, DNA extraction, screening, and PCR are performed using cultured samples obtained from milk and other sources, allowing for the simple detection of Mycoplasma genes. The Plus Kit series has adopted the PCR supplement Plus, which suppresses PCR inhibition by samples, resulting in improved detection sensitivity compared to conventional products. This kit series is developed by Dr. Goki Higuchi and Dr. Eichi Iwano from Rakuno Gakuen University, applying a rapid and easy measurement technique using PCR. *This product is a reagent for testing and research purposes. 【Features】 ● Capable of detecting the main Mycoplasma genus genes present in livestock animals (does not detect Acholeplasma or Ureaplasma genera, etc.) ● Can be detected quickly and easily by PCR (approximately 3 hours) ● Improved detection sensitivity compared to conventional products.

The "Kogene Biotech Gene Detection Kit Series" includes over 500 real-time PCR/conventional PCR kits for detecting pathogens such as monkeypox, novel coronavirus variants, and pathogenic bacteria. 【Product Lineup】 - Pathogenic Bacteria Salmonella, Shigella, Vibrio parahaemolyticus, Legionella, etc. - Enteric Viruses Adenovirus, Norovirus, Hepatitis A/C viruses, etc. - Respiratory Viruses and Bacteria Measles virus, Coronavirus, Chlamydia pneumoniae, etc. - Influenza Viruses Influenza A/B viruses, Avian influenza, etc. - Protozoa Entamoeba histolytica, Giardia lamblia, etc. - Tropical Disease Viruses Dengue virus, Zika virus, etc. - Tick-borne Infection Pathogens Rickettsia, Bartonella, etc. - Veterinary Medicine BVDV, PEDV, Mycobacterium avium, etc. - Animal Species Chickens, sheep, ducks, etc. - Food Allergens Shrimp & crab, soybeans, peanuts, etc. - Halal For detecting gelatin-derived DNA - Genetically Modified Organisms (GMO) - Plant Pathogenic Viruses ToMV, RSV, etc.

This product is a one-step RT-PCR kit (real-time PCR method) that can specifically detect the genes of the Orthopoxvirus and the Mpox virus. It enables rapid and accurate virus detection. ■ Specifically detects the genes of Orthopoxvirus and Mpox virus ■ All necessary reagents, including internal controls and positive controls, are included ■ Compatibility with widely used real-time PCR devices has been confirmed ★ Fluorescent channels used for detection FAM, JOE (VIC or HEX), Cy5 ★ This kit is intended for research and testing purposes only. It has not been approved or certified as an in vitro diagnostic medical device.

Halal means "permitted" in Arabic. Halal is a very important discipline for Muslims, and there is an increasing need to distinguish between halal and non-halal. Among them, pork is a representative example of non-halal, and it is required to check whether pork and its derivatives are included in food, pharmaceuticals, cosmetics, and so on. The PowerChek halal testing real-time PCR kit can accurately conduct halal testing with high precision by using primers specific to pig DNA.

Mycoplasma spp. is a type of true bacteria known to cause various diseases such as pneumonia, otitis media, arthritis, and mastitis in production animals like cattle. In this third seminar, titled "Clinical Aspects of Bovine Mycoplasma Infection," we will hear presentations on insights and responses from the clinical field.

The Shikajinius series has added a real-time PCR kit! 【Features】 ● It can simultaneously detect the vancomycin resistance genes vanA, vanB, and vanC. ● An internal control allows for confirmation of the presence or absence of PCR inhibition. ● Detection is possible in about 1 hour using real-time PCR.