KANTO CHEMICAL CO., INC. List of Products and Services

We offer Shikajinius nucleic acid extraction reagents and nucleic acid purification kits according to the purpose of extracting nucleic acids (DNA/RNA) from samples. The nucleic acid extraction reagent is a simple purification reagent that allows for PCR preprocessing in as little as 10 minutes. The nucleic acid purification kit is a silica membrane-type purification kit that can be selected from various types depending on the sample type. *Please check the selection guide for choosing the appropriate kit based on the sample. If it is difficult to determine the suitable kit for your sample, please feel free to contact us. *Samples of each product are also available. You can apply through the application form in the selection guide or contact us directly from this site.

This reagent promotes the binding of miRNA to the spin column carrier by adding it to a buffer containing chaotropic salts. By combining this reagent with the Sicagenius RNA Prep Kit (for tissue) instead of using a dedicated kit, it becomes possible to efficiently extract miRNA.

"Protease K solution" is a reagent that efficiently breaks down proteins due to its broad cleavage activity as a type of endopeptidase. 【Features】 ■ Optimal for nucleic acid purification with DNase and RNase below detection limits ■ Recombinant enzyme with minimal lot-to-lot variation ■ Solution type that is easy to dilute ■ Guaranteed enzyme activity of ≧600 U/mL (37 °C, hemoglobin) ■ Long-term storage possible even in refrigeration (2–8 °C) *For more details, please refer to the catalog or feel free to contact us.

"Agarose KANTO" is a purified agarose for the electrophoresis of nucleic acids such as DNA. You can adjust the agarose concentration according to the DNA size for the separation and recovery of nucleic acids. You can choose from seven types of agarose suitable for electrophoresis, and we offer high-quality materials at an affordable price. [Features] ■ Easy-to-handle solubility and gel strength ■ High cost performance ■ A range of products tailored for various applications *For more details, please refer to the catalog or feel free to contact us.

The 'Agar for Dryness Prevention' is an agar that is highly resistant to drying and has excellent water retention, making it ideal for algae, plants, and microorganisms. By using this agar, it is possible to prevent cracking of the medium and drying of samples that can occur during long-term cultivation due to dryness. *For more details, please refer to the catalog or feel free to contact us.

"LB Medium (Miller), Granulated Type" is a premix type of LB medium. It is ideal for the cultivation of competent cells and others. 【Features】 ■ Granulated type that minimizes powder scattering *For more details, please refer to the catalog or feel free to contact us.*

The "phi29 DNA Polymerase Set" is a DNA synthesis enzyme derived from the Bacillus subtilis phage phi29. It is active around 30°C, possesses high strand displacement activity, and has excellent proofreading activity due to its 3'→5' exonuclease activity, making it suitable for applications such as whole genome amplification. Our unique manufacturing technology allows for the extreme removal of residual DNA. 【Features】 ■ Mesophilic enzyme ■ High strand displacement activity ■ Fast synthesis speed ■ High elongation capability ■ Low replication error rate For more details, please refer to the catalog or feel free to contact us.

In the field of life sciences, there is a demand for optical manipulation techniques that achieve high temporal and spatial resolution and allow for precise control that is difficult to achieve with drug administration, as a means of functional analysis of genes and the creation of disease models. This product is a light-inducible Cre recombinase (Photoactivatable Cre; PA-Cre) that combines Cre recombinase with the light-switchable protein Magnet System. By using this product, it becomes possible to control the function of genes in a time- and space-specific manner through light stimulation.

The "QIAxcel Connect" is a capillary electrophoresis device from QIAGEN that serves as an alternative to DNA/RNA gel analysis. It allows for safe and easy analysis using ready-to-use gel cartridges. The electrophoresis results can be output as digital data, which is convenient for analysis. Additionally, related products include the Sicagenius Molecular Epidemiology Analysis POT Kit series and Sicagenius PCR kits, among others. 【Features】 ● High-speed analysis of up to 96 samples without manual operation ● Accurate results even at nucleic acid concentrations of 5 pg/μL ※1 ● Analysis of DNA fragments from 12 samples (one run) in 9-25 minutes ※2 ● Accurate analysis with high resolution of 3-5 bp (for fragments under 500 bp) ※2 ● Safe and easy system with ready-to-use gel cartridges ● Output of convenient digital data for analysis ※1 High-sensitivity detection reagents are scheduled to be released soon. ※2 When using the QIAxcel DNA High Resolution Kit. ※For more details, please refer to the catalog or feel free to contact us.

"SCOPE 21" is a desktop imaging device manufactured by OPTIMA Corporation. It comes with a variety of accessories, including a digital camera (requires an SDHC card) and filters for different staining solutions. Additionally, the digital camera is pre-configured for gel imaging.

CBB staining (Coomassie Brilliant Blue staining) is commonly used for protein staining after electrophoresis, but when preparing CBB staining solution in-house, it can be time-consuming due to the need for prolonged dissolution and filtration. Additionally, skipping the filtration step can lead to aggregated particles, causing uneven staining and reducing staining and decolorization efficiency, which takes more time. Kanto Chemical has improved the traditional CBB staining solution, offering a pre-prepared staining solution that enhances detection sensitivity and increases staining and decolorization speed. A two-component type that is resistant to changes over time and allows for MS analysis after decolorization → Rapid CBB KANTO A ready-to-use type that improves speed, sensitivity, and safety → Rapid CBB KANTO 3S *For more details, please refer to the catalog or feel free to contact us.

The "NanoEX Plus/Lite" is an ultra-micro spectrophotometer manufactured by OPTIMA Corporation. It allows measurements without the need for a computer and can be used in various locations. Additionally, the automatic setting of the optical path length enables the omission of blank measurements. 【Features】 ● Measurement possible in 2 steps (drop → measurement) by omitting blank measurement ● Standalone type that does not require a computer and has a compact size ● Various functions can be easily operated via an LED touch panel ● Three wavelength measurements at 260, 280, and 600 nm (glass cell) with baseline correction at 360 nm: NanoEX Plus ● Full spectrum measurement in the UV-visible range is possible (NanoEX Lite) *For more details, please refer to the catalog or feel free to contact us.

In the field of biotechnology, particularly in the research of cell control, technologies that precisely regulate gene functions are essential. Light manipulation techniques that enable temporal and spatial control, which are difficult to achieve with drug administration, are in demand. This product is PA-Cre, which combines the Cre recombinase with the light-switchable protein Magnet System. With this product, the activity of genes can be controlled by light stimulation. 【Application Scenarios】 - Cell culture experiments requiring temporal and spatial control of gene expression - Gene function analysis in specific cell types or tissues - Gene manipulation in the creation of disease models 【Benefits of Implementation】 - Activation of Cre recombinase by light irradiation allows for on/off control of gene expression - Precise gene control with high temporal and spatial resolution - More accurate gene manipulation compared to drug administration *This product is a reagent for testing and research purposes. It cannot be used for human or animal pharmaceuticals, quasi-drugs, medical devices, regenerative medicine products, in vitro diagnostic drugs, or any other purposes outside of testing and research.