関東化学株式会社 List of Products and Services

Kanto Chemical has launched a new manufacturing service for media grades that can be used for clinical research and regenerative medicine products, leveraging the know-how accumulated over many years as a comprehensive reagent manufacturer. We will respond carefully to our customers' requests, so please feel free to consult with us. 【Features】 ■ Manufacturing and quality management based on the ISO 9001 quality management system ■ Strong procurement capabilities for raw materials and product development and proposal backed by years of experience in the reagent business ■ Use of self-manufactured water for injection (WFI) grade water for manufacturing ■ Support for consultations on the material suitability of regenerative medicine products is also available Please feel free to contact us for details or quotes.

This product is an animal-free medium that does not contain human or animal-derived components, making it suitable for regenerative medicine research. By using this product, it is possible to proliferate human mesenchymal stem cells while maintaining their properties. Additionally, we have obtained a confirmation of material suitability for regenerative medicine products from the PMDA (Pharmaceuticals and Medical Devices Agency), confirming compliance with biological material standards. 【Features】 ■ Animal-free medium for mesenchymal stem cells (MSC) - does not contain human-derived components ■ High proliferation support capability ■ Obtained confirmation of material suitability for regenerative medicine products Data on cell morphology, proliferation, differentiation ability, undifferentiated state, and adaptability are included in the brochure, so please refer to it. - This product was developed using collaborative research results from the Department of Orthopaedic Surgery at the University of Tokyo and Avenue Medical Corporation. - This product is a medium for the proliferation of mesenchymal stem cells (MSC). In the production of TOPs cells, it is necessary to use not only this product but also the tissue collection and culture methods developed through collaborative research between the Department of Orthopaedic Surgery at the University of Tokyo and CPC Corporation.

The "ciKIC iPS medium" is a feeder-free medium suitable for the culture of iPS cells. Typically, frequent medium changes are necessary when culturing iPS cells, but using this medium eliminates the need for medium changes on weekends (or during three-day holidays). Since a certain number of cells can be seeded, it allows for simple and highly reproducible passaging work. 【Features】 ■ Low-protein medium without albumin ■ Reduced burden of medium changes ■ Passaging work possible with single cells ■ High proliferation support capability ■ Good ability to maintain undifferentiated state ■ Free from components of animal origin (Xeno-free) ★ For applications (cell morphology, proliferation, karyotype analysis, undifferentiated state, pluripotency, etc.), please refer to the brochure.

The "ciKIC IEC Maturation Medium" is a culture medium that allows for the maturation of intestinal epithelial cells derived from human iPS cells, enabling the creation of a human intestinal epithelial model. In oral drug pharmacokinetic testing, the Caco-2 cells derived from human colon cancer have traditionally been used as a model for gastrointestinal absorption and metabolism tests; however, there has been a challenge in adequately replicating the functions of the living small intestine. This product allows for the maturation of intestinal epithelial cells derived from human iPS cells, making it possible to create a human intestinal epithelial model that is similar to the living small intestine. 【Features】 ■ Capable of producing high-performance human intestinal epithelial models ■ Wide range of applications *For more details, please refer to the PDF materials or feel free to contact us. 【Keywords】 Drug discovery support, non-clinical trials, pharmacokinetics, gastrointestinal absorption tests, Caco-2, iPS-small intestine model, drug metabolism enzymes, transporters, barrier function, drug-drug interactions, functional foods, health foods, nutrient absorption, intestinal immunity, gut microbiota.

BioConcept is a manufacturer of cell culture media based in Basel, Switzerland. The company specializes in the production of animal-free (ACF) media, with its manufacturing facility equipped with ACF-specific containers and production equipment. To maintain high product quality, it utilizes a very high standard of water purification systems and air conditioning control systems. Additionally, to meet various customer needs, the equipment and control mechanisms in the manufacturing facility are regularly upgraded. Custom media and other flexible solutions are available. 【About Custom Media】 The following items can be customized: ● Changes to the composition of existing products (addition or removal of components), concentration changes ● Contract manufacturing of original formulation media ● Changes in batch size (liquid: 20 L to 5,000 L, powder: 2 kg to 800 kg) ● Changes in container size and type ● Changes in testing content Standard tests: pH, osmotic pressure, concentration, sterility test (liquid products only), cell culture performance test Additional possible items: conductivity, endotoxin, mycoplasma, bioburden (powder products only), etc. *For more details, please refer to the PDF document or feel free to contact us.

The "MAM-PF Medium Series" is a culture medium for CHO cells and BHK cells that meets the standards of being serum-free, protein-free, and free from animal-derived components (ACF). The liquid medium uses high-quality water for injection (WFI) and the particle size of the powdered medium is crushed to approximately 20 µm, enabling rapid dissolution. Additionally, it allows for high-density cell culture and facilitates high expression of recombinant proteins. 【Features】 ■ Chemically defined and free from animal-derived components ■ Compliant with European Medicines Agency EMA/410/01 ■ High cell density and high yield ■ High-quality liquid and powdered media ■ High adaptability *For more details, please refer to the PDF document or feel free to contact us.

Human mesenchymal stem cells (hMSC), one type of somatic stem cell, possess the ability to differentiate or transdifferentiate into cartilage, bone, ligaments, fat, muscle, and blood vessels, and can be isolated from living tissues such as bone marrow with minimal discomfort. Therefore, they are attracting attention as transplantable stem cells for regenerative medicine. Traditionally, the in vitro amplification and differentiation induction of MSCs required fetal bovine serum (FBS) or autologous human serum (derived from the patient's own blood), but the STK series allows for serum-free culture of MSCs and is composed of chemically defined media, making it a useful tool for future MSC research. *For more details, please refer to the PDF document or feel free to contact us.*

PepTigrowth's "Growth Factor Alternative Peptides" are produced through complete chemical synthesis without the use of biological or cellular materials. Compared to conventional growth factors and cytokines, they possess equivalent activity and high stability, and we can provide a certificate of non-use of biological raw materials. This contributes to research and development in regenerative medicine and cell therapy. 【Features】 ■ Completely chemically synthesized product ■ Equivalent activity to conventional recombinant proteins per mol concentration ■ High stability ■ Certificate of non-use of biological raw materials available ■ Stable supply through domestic production *For more details, please refer to the PDF document or feel free to contact us.

APExBIO is a manufacturer based in Texas, USA, that produces and sells bioactive molecules and molecular biology kits. Among the approximately 8,000 types of bioactive molecules the company offers, we have prepared a selection of key bioactive molecules useful for stem cell research. For products from APExBIO not listed in the brochure, please contact Kanto Chemical separately. 【Features】 ● High Purity Many items with a purity of over 99% ● Comprehensive Product Information Detailed physical property data, examples of cell and animal experiments, and references can be found on the manufacturer's website. In our brochure, you can check the applications of each substance for stem cell research, including "reprogramming, maintenance of undifferentiated state, and differentiation induction." * We offer a wide range of items for targeted signal transduction and cellular functions. For more details, please refer to the PDF materials or feel free to contact us.

MatriMix is a new three-dimensional culture substrate composed of collagen, laminin E8, and hyaluronic acid. By varying the types, combinations, and concentrations of collagen and laminin E8, it provides a microenvironment suitable for various cells, promoting tissue formation. 【Features】 ■ New three-dimensional culture substrate ■ Clear composition of reagent components ■ Applicable to various culture methods ■ Suitable for organ tissue engineering that mimics the in vivo environment *For more details, please refer to the PDF document or feel free to contact us.

Fibronectin Neosilk, Plasma and Fibronectin Neosilk, Cellular from Immunobiological Research Institute Co., Ltd. are recombinant plasma fibronectin and cellular fibronectin produced using silkworms that do not carry human infectious pathogens. They can be used as coating agents for various cultured cells, including mesenchymal stem cells (MSCs).

In the field of biochemistry research and experiments, buffers play an important role. Currently, we offer a variety of reagents for buffer preparation, including widely used Tris and HEPES, which has minimal impact on living organisms. ●Example of a product 【Special Grade 2-Amino-2-Hydroxymethyl-1,3-Propanediol (Tris)】 In the field of biochemistry, "2-Amino-2-Hydroxymethyl-1,3-Propanediol (abbreviated: Tris)" is utilized as a buffer. We have prepared a product of "special" grade that includes "absorbance (260 nm, 280 nm)" and "appearance" as guaranteed items, in addition to the "superior" grade specifications. This product emphasizes a balance between quality and cost required for research and development in biochemistry, enhancing cost performance. 【Features】 ■ Guarantees "absorbance (260 nm, 280 nm)" and "appearance" in addition to superior grade specifications. ■ High cost performance with a focus on the balance between quality and cost. ■ Capable of scaling up from lab scale. * For specific supply quantities, please consult us for details.