日研ザイル List of Products and Services

At Nikken Zail Co., Ltd., we conduct contract analysis using biomarkers for health management, aging control research, and food development. Through various tests, it is possible to analyze the oxidative stress state in the body from multiple perspectives, including oxidative stress levels, antioxidant capacity against reactive oxygen species, and the sufficiency of lipid-soluble/water-soluble antioxidants that reduce oxidative damage. We offer a range of highly original test items, such as "8-OHdG analysis" to measure DNA oxidative damage and "hexanoyl lysine analysis" to measure lipid peroxidation. 【Test Items (Partial)】 ■Oxidative Damage ■Oxidative Precursors ■Antioxidant Capacity ■Water-Soluble Antioxidants ■Lipid-Soluble Antioxidants *For more details, please download the PDF or feel free to contact us.

• Contract Inspection

We would like to introduce our antioxidant/enzyme measurement kit, the "Serum Myeloperoxidase (MPO) Measurement Kit." This kit measures serum/plasma MPO using a sandwich ELISA method with anti-human MPO antibodies. MPO has been reported to be present in atherosclerotic lesions and is involved in the mechanisms of inflammation and cardiovascular disease development. 【Product Specifications (Partial)】 ■ Measurement Target: Myeloperoxidase in human serum or plasma ■ Measurement Range: 1.9–30 ng/mL ■ Time Required: Approximately 2.5 hours ■ Sample Volume Required: Approximately 25 μL (100 μL for plasma) ■ Capacity: 96 wells *For more details, please download the PDF or feel free to contact us.

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We would like to introduce the glycation stress and glycation markers handled by Nikken Zail Co., Ltd. Glycated albumin (Glyco Albumin: GA) in serum is gaining attention as an excellent glycation marker that reflects fluctuations in blood glucose levels over a shorter period than HbA1c. Serum albumin is more susceptible to glycation modification compared to hemoglobin and reflects postprandial blood glucose fluctuations. 【Product Lineup】 ■ Anti-MG monoclonal antibodies ■ Mouse glycated albumin measurement kit ■ Rat glycated albumin measurement kit *For more details, please download the PDF or feel free to contact us.

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We would like to introduce our protein oxidation damage marker, the "Carbonylated Protein Measurement Kit (for Human/Animal Use)." After reacting the carbonylated proteins present in the sample with DNP, it is possible to measure carbonylated proteins with high sensitivity by using antibodies specific to the proteins bound to DNP. The required time is approximately 2 hours for sample preparation and about 4 hours (or overnight plus 1 hour) for ELISA measurement. 【Product Specifications (Partial)】 ■ Measurement targets: Plasma, bronchoalveolar lavage fluid, cerebrospinal fluid, cell extracts, etc., derived from humans and animals ■ Measurement range: 156–2500 pmol/mg protein ■ Sample volume required: Approximately 4 μL ■ Capacity: 96 wells *For more details, please download the PDF or feel free to contact us.

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The "Plasma Nitrotyrosine Measurement Kit" is an ELISA kit that allows for the simple measurement of dityrosine in samples. Dityrosine is formed as an intermediate through tyrosyl radicals by ultraviolet light, radiation, hydrogen peroxide, and peroxidase. It is detected not only in atherosclerotic lesions but also in connective tissues such as collagen and elastin. Dityrosine is also detectable in urine and is expected to serve as a new non-invasive protein oxidation marker. 【Product Specifications (Partial)】 ■ Measurement Targets: Serum, plasma, feces (applicable to both humans and animals) ■ Specificity: Nitrolysed modified proteins ■ Measurement Range: 55.5 to 1500 nM ■ Time Required: Approximately 4 hours ■ Sample Volume Required: 50 μL *For more details, please download the PDF or feel free to contact us.

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We would like to introduce our lipid oxidation damage marker, the "Isoprostane Measurement Kit." By measuring isoprostanes in urine, it is possible to non-invasively evaluate oxidative stress (lipid oxidation) in the body. This product is an ELISA kit designed to measure 15-isoprostane F2t in urine samples. 【Product Specifications (Partial)】 ■ Measurement Principle: Competitive ELISA Method ■ Measurement Target: Urinary Isoprostanes ■ Measurement Range: 0.05 to 100 ng/mL ■ Time Required: Approximately 3 hours ■ Sample Volume Required: Approximately 100 μL *For more details, please download the PDF or feel free to contact us.

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The "8-OHdG Check ELISA" is an ELISA kit that uses a monoclonal antibody (N45.1) specific to 8-hydroxy-2'-deoxyguanosine. It allows for easy and sensitive measurement of 8-OHdG in urine, serum, and tissue DNA. It reacts very little with the RNA oxidant 8-hydroxy-guanosine (8-OHG) and is characterized by its ability to specifically detect the DNA oxidant (8-OHdG). In particular, the "New 8-OHdG Check" is designed for urinary 8-OHdG and can measure multiple samples of urinary 8-OHdG within 3 hours without any pretreatment. 【Product Specifications (Excerpt)】 ■ Measurement Range: 0.5 to 200 ng/mL ■ Specificity: Specifically detects 8-OHdG ■ Capacity: 96 wells for 18 samples (N=3 measurements) ■ Storage Conditions: Refrigerated (2 to 8°C); please use within 2 weeks after opening *For more details, please download the PDF or feel free to contact us.

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Our company offers "oxidative stress marker contract testing." The measurement targets include oxidative damage to DNA, oxidative damage to cell membranes, lipid peroxidation levels, antioxidants, and antioxidant capacity. We conduct contract testing for each marker, so for details on contract testing and other markers, please contact our representative. 【Measurement Targets】 ■Oxidative damage to DNA ■Oxidative damage to cell membranes ■Lipid peroxidation levels ■Antioxidants ■Antioxidant capacity *For more details, please download the PDF or feel free to contact us.

• Contract Inspection

The "Antioxidant Capacity Measurement Kit PAO" is an oxidative stress marker that utilizes the reduction reaction of copper ions to measure the antioxidant capacity of samples quickly and easily. It shows a high correlation with the oxidation lag time of serum and is also applicable for evaluating the antioxidant capacity of food samples such as red wine and black tea. It can be used to assess the total antioxidant capacity of serum. 【Features】 ■ Detects antioxidant substances based on their reducing power against copper ions ■ Broadly detects both water-soluble and fat-soluble antioxidant compartments ■ Can be easily measured in about 5 minutes ■ Measurable in both food and serum *For more details, please download the PDF or feel free to contact us.

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Reactive oxygen species generated under oxidative stress react with biomacromolecules such as DNA, lipids, proteins, and enzymes in the body, resulting in lipid peroxidation, DNA mutations, protein denaturation, and enzyme inactivation. In recent years, it has become clear that oxidative stress is deeply involved in numerous diseases, including lifestyle-related diseases such as aging, diabetes, and hypertension, and that reactive oxygen species themselves play important physiological roles in signal transduction and immune mechanisms. Accurately assessing oxidative stress in the body and implementing measures to reduce oxidative stress are expected to be helpful for understanding disease conditions, diagnosing pre-disease, preventing diseases, and controlling aging. 【Related Products】 ■ New 8-OHdG Check Kit ■ High Sensitivity 8-OHdG Check Kit ■ Antioxidant Capacity Measurement Kit PAO *For more details, please download the PDF or feel free to contact us.

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We offer the "High Sensitivity 8-OHdG Check Kit," an oxidative stress marker that allows for easy and sensitive measurement of 8-OHdG in organizational DNA. The high sensitivity type is suitable for measuring 8-OHdG in serum and tissues. This kit is applicable to most animal species, including humans, mice, rats, hamsters, rabbits, dogs, cattle, and horses. 【Features】 ■ Easily and sensitively measure 8-OHdG ■ Capable of detecting oxidative damage in the body sensitively ■ Applicable to most animal species *For more details, please download the PDF or feel free to contact us.

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The "New8-OHdG Check Kit" is an ELISA kit that uses monoclonal antibodies specific to 8-hydroxy-2'-deoxyguanosine. It allows for easy and sensitive measurement of 8-OHdG in urine, with minimal reaction to the RNA oxidant 8-hydroxyguanosine (8-OHG), specifically detecting the DNA oxidant (8-OHdG). Additionally, it enables the measurement of urinary 8-OHdG from multiple samples within 3 hours without any pre-treatment. 【Features】 - Easily measure the level of oxidative damage in the body using urine - Results available in approximately 3.5 hours - Simultaneous measurement of multiple samples possible (1 kit, N=3 measurements can test 18 samples) *For more details, please download the PDF or feel free to contact us.

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We provide contract analysis using biomarkers for health management, aging control research, and food development. We offer a variety of highly original test items, such as "8-OHdG analysis" to measure DNA oxidative damage and "hexanoyl lysine analysis" to measure lipid peroxidation. These tests allow for a multifaceted analysis of the oxidative stress state in the body, including the level of oxidative stress, antioxidant capacity against reactive oxygen species, and the sufficiency of lipid-soluble/water-soluble antioxidants that reduce oxidative damage. We aim to provide reliable test data quickly to support the research of medical institutions, companies, and research organizations. 【Features】 ■ Contract analysis using biomarkers ■ Highly original test items ■ Quick provision of reliable test data ■ Support for the research of medical institutions, companies, and research organizations *For more details, please refer to the PDF document or feel free to contact us.

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This product is expected to serve as a new oxidative stress biomarker that can capture the early stages of lipid peroxidation, unlike conventional aldehyde-based lipid peroxidation markers such as 4-HNE (4-hydroxy-2-nonenal) and MDA (malondialdehyde). Regarding its association with diseases, it has been immunologically proven to localize in human atherosclerotic lesions, and the generation of HEL in muscle in a rat exercise model, as well as the inhibitory effects of flavonoids on its production, have been clarified. 【Features】 ■ Simple quantification of hexanoyl lysine adducts in biological samples ■ Usable for research and evaluation of oxidative stress in vivo, particularly in the early stages of lipid peroxidation ■ Capable of assessing lipid-related oxidative stress in various samples such as urine, serum, and cultured cells *For more details, please refer to the PDF document or feel free to contact us.

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The "Urinary Isoprostane Measurement Kit" is an ELISA kit designed to measure 15-isoprostane F2t (15-isoprostane F2t: 8-epi-PGF2α/8-isoPGF2α) in urine samples. By measuring urinary isoprostanes, it is possible to non-invasively assess oxidative stress (lipid oxidation) in the body. Additionally, we also offer "Urinary Isoprostane Contract Testing" that can accommodate animal samples. *For more details, please refer to the PDF document or feel free to contact us.

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The 『KGA-051K』 is an ELISA kit designed to detect glycosylated albumin (glycoalbumin) in rat serum, plasma, and cultured cells. In recent years, it has been suggested that the rapid increase in blood glucose levels after meals plays an important role in the pathology of atherosclerotic lesions caused by oxidative stress and associated endothelial damage. Glycosylated albumin (glycoalbumin: GA) in serum is gaining attention as an excellent glycation marker that reflects fluctuations in blood glucose levels over a shorter period than HbA1c. In addition to this, we also offer a "Mouse Glycosylated Albumin Measurement Kit," as well as "Glycosylated Albumin Contract Analysis" and "1.5-AG Contract Analysis (1.5-Anhydroglucitol)." *For more details, please refer to the PDF document or feel free to contact us.

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The "Oxidized LDL Measurement Kit" is a lipid oxidation damage marker that can measure oxidized LDL in human serum/plasma samples. The measurement principle is the sandwich ELISA method. The required time is approximately 5.5 hours. *For more details, please refer to the PDF document or feel free to contact us.

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"PAO" is an antioxidant measurement kit that utilizes the reduction reaction of copper ions (Cu+ + → Cu+) to conveniently measure the antioxidant capacity of samples in a short time (about 5 minutes). It not only shows a high correlation with the oxidation lag time of serum but is also applicable for evaluating the antioxidant capacity of food samples such as red wine and black tea. *For more details, please refer to the PDF document or feel free to contact us.

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The "Plasma Nitrotyrosine Measurement Kit" is a protein oxidation damage marker that can measure nitrosylated modified proteins using the sandwich ELISA method. Nitrotyrosine (3-nitrotyrosine: 3-NT) is one of the major protein nitrosylation modifications caused by peroxynitrite and is widely used as a marker of nitrosative stress. *For more details, please refer to the PDF document or feel free to contact us.*

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The "Serum Myeloperoxidase (MPO) Measurement Kit" is an antioxidant/enzyme measurement kit that can measure serum/plasma MPO using a sandwich ELISA method with anti-human MPO antibodies. MPO concentrations vary significantly between serum and plasma. It is also known that the plasma MPO concentration differs depending on the type of anticoagulant used, with heparin plasma and citrate plasma showing higher values compared to EDTA plasma (Shih et al. Clin Chem 2008). Please choose the sample collection and preparation method according to your research purposes. *For more details, please refer to the PDF materials or feel free to contact us.

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The "Paraoxonase Activity Measurement Kit" is a redox/anti-aging related marker that allows for the measurement of paraoxonase in samples by detecting paraoxonase activity (paraoxon hydrolysis activity) in the sample. Paraoxonase (PON-1) is an enzyme that exists in the blood bound to apolipoprotein A1 of HDL (High-Density Lipoprotein) and acts as an antioxidant enzyme that demonstrates protective effects against lipoprotein oxidation. There have been reports of its association with various diseases, including atherosclerosis, and it has been reported that serum paraoxonase activity shows low values in conditions such as obesity, diabetes, metabolic syndrome, hyperlipidemia, and iron deficiency anemia. *For more details, please refer to the PDF document or feel free to contact us.*

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The "Dityrosine (DT) Measurement Kit" is an ELISA kit that allows for the simple measurement of dityrosine in samples. Dityrosine is a dimer of tyrosine formed by the oxidation of tyrosine in proteins or free tyrosine. It is formed with tyrosyl radicals as intermediates through ultraviolet light, radiation, hydrogen peroxide, and peroxidase. In addition to being detected in atherosclerotic lesions, it has also been found in connective tissues such as collagen and elastin. Dityrosine can also be detected in urine and is expected to be a new protein oxidation marker that can be measured non-invasively. *For more details, please refer to the PDF document or feel free to contact us.*

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The "Glutathione Measurement Kit" is a kit for measuring total glutathione (oxidized + reduced) in samples derived from animals and plants. Glutathione in the sample reacts with DTNB (5-5'-dithiobis[2-nitrobenzoic acid]) to form TNB (5-thiobenzoic acid) and GS-TNB, which have an absorption at 412 nm. GS-TNB is broken down into TNB and GSH through a cyclic reaction by glutathione reductase, and this GSH reacts again with DTNB to produce TNB. By measuring the change in absorbance at 412 nm (or 405 nm), glutathione can be detected with high sensitivity. Additionally, this kit not only measures total glutathione but also allows for the evaluation of the oxidized (GSSG) / reduced (GSH) ratio by pre-treating the sample with 4-vinylpyridine to detect only oxidized glutathione. *For more details, please refer to the PDF document or feel free to contact us.*

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The "Carbonylated Protein Measurement Kit" is a marker for protein oxidative damage in humans and animals. Traditionally, colorimetric methods and HPLC methods utilizing the reaction with dinitrophenylhydrazine (DNPH) have been used to measure carbonylated proteins, but there have been issues such as the need for a large sample volume. This kit allows for the high-sensitivity measurement of carbonylated proteins by reacting the carbonylated proteins in the sample with DNPH and then using a specific antibody that binds to the DNPH-conjugated proteins. 【Features】 ■ Reacts carbonylated proteins in the sample with DNPH ■ Uses a specific antibody for DNPH-conjugated proteins ■ Measures carbonylated proteins *For more details, please refer to the PDF document or feel free to contact us.

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The "SOD Activity Measurement Kit" is a kit for measuring the scavenging activity of superoxide. The measurement principle is based on the automatic oxidation suppression reaction of hematoxylin. In the presence of oxygen, hematoxylin (HTH) undergoes automatic oxidation, resulting in an absorption at 560 nm. Since this automatic oxidation is suppressed by SOD, the amount of SOD activity in the sample is calculated based on the suppression rate of absorbance at 560 nm. 【Features】 ■ Measures the scavenging activity of superoxide ■ Calculates the amount of SOD activity in the sample based on the suppression rate of absorbance *For more details, please refer to the PDF document or feel free to contact us.

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The "Measurement ELISA Kit (New 8-OHDG Check ELISA)" is a marker for DNA oxidative damage. This product is an ELISA kit that uses a specific monoclonal antibody for 8-hydroxy-2-deoxyguanosine (8-OHdG/8-oxo-dG). It allows for easy and sensitive measurement of 8-OHdG in urine, serum, and tissue DNA. Additionally, it is designed for urinary 8-OHdG, enabling multi-sample measurement of urinary 8-OHdG without pretreatment in just 3 hours. 【Features】 ■ ELISA kit using specific monoclonal antibodies ■ Easily and sensitively measures 8-OHdG in urine, serum, and tissue DNA ■ Designed for urinary 8-OHdG ■ Allows multi-sample measurement of urinary 8-OHdG without pretreatment in 3 hours *For more details, please refer to the PDF document or feel free to contact us.

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The "MDA Measurement Kit (TBARS Method)" is a marker for measuring lipid oxidation damage through the detection of malondialdehyde. This product reacts malondialdehyde in the sample with thiobarbituric acid (TBA) to form the MDA-TBA2 adduct. Since this adduct has a strong absorption near a wavelength of 532 nm, it allows for the spectroscopic detection of MDA. 【Features】 ■ A marker for measuring malondialdehyde as a lipid oxidation damage indicator ■ Reacts malondialdehyde with thiobarbituric acid (TBA) ■ Forms the MDA-TBA2 adduct ■ Spectroscopically detects MDA *For more details, please refer to the PDF document or feel free to contact us.

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This is a comprehensive product catalog published by the Japan Aging Control Research Institute, featuring a selection guide for "oxidative stress markers." In the Nikkanzail kit reagent business, we offer various kits for measuring oxidative stress markers. You can easily view the marker names/product names by category, such as DNA oxidative markers and lipid oxidative markers, as well as applicable samples and specifications, along with detailed introductions for each product. 【Categories】 ■ DNA oxidative markers ■ Lipid oxidative markers ■ Glycation markers ■ Protein oxidative markers ■ NO stress, etc. *For more details, please refer to the PDF materials or feel free to contact us.

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Nikken Zail Co., Ltd. primarily engages in the development, manufacturing, import, export, and sales of research reagents, while the Japan Anti-Aging Research Institute conducts kit reagent business and contract testing services. In the kit reagent business, we develop, manufacture, and sell research reagents for measuring oxidative stress markers. In the contract testing business, based on the results of product development and years of accumulated analytical technology, we conduct specimen testing of urine and blood, as well as evaluate the antioxidant properties of food. Our company focuses on anti-aging research and aims to utilize our research results for the benefit of everyone's health. 【Business Activities】 ■ Development, manufacturing, import, export, and sales of research reagents ■ Specimen testing and food testing business (Middle East Health Protection No. 16-5) *For more details, please refer to our catalog or feel free to contact us.

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