重松貿易 List of Products and Services

"OpeRATOR" is a specific protease that cleaves the N-terminal residues of Ser and Thr in glycan residues of proteins with mucin-type O-linked glycans. O-linked glycopeptides are generated, allowing for O-linked glycan profiling, peptide mapping, determination of site occupancy, and intermediate-level approaches through MS analysis. 【Features】 ■ Specifically cleaves proteins with O-linked glycans ■ Generates O-linked glycopeptides ■ Enables O-linked glycan profiling, peptide mapping, determination of site occupancy, and intermediate-level approaches through MS analysis ■ Does not digest glycoproteins at N-linked glycan sites *For more details, please refer to the PDF document or feel free to contact us.

"GingisREX (RgpB)" is an arginine-specific protease that cleaves proteins at the C-terminal of arginine residues. It is capable of cleaving arginine-proline sequences, which are difficult to cleave with other enzymes. This enzyme is useful for peptide mapping, de novo peptide sequencing, and the analysis of post-translational modifications. 【Features】 - Cleavage is possible even when the adjacent amino acid is proline. - Compared to cleavage by trypsin, longer peptides are generated, allowing for separation and identification using high-resolution MS. - Increases options for sample preparation for bottom-up approaches, improving degradation profiles and sequence coverage. - Shows activity over a wide pH range of 5.0-9.0, requires cysteine, but is inhibited by guanidine hydrochloride. *For more details, please refer to the PDF document or feel free to contact us.*

TransGLYCIT is a platform technology for efficiently transglycosylating native human IgG within 3 hours. Using enzymatic remodeling, it enables the transglycosylation of N-linked glycans in the Fc region of antibodies, resulting in a robust workflow that allows for the preparation of antibody products with specific glycoforms. This technology is available in kit form for producing antibodies with G0, G1, G2, or G2S2 glycan profiles (± fucose), and there is also an option to remove fucose from the core GlcNAc using the TransGLYCIT Afucosylated kit. 【Features】 ■ Efficiently transglycosylate native human IgG within 3 hours ■ Allows for the preparation of antibody products with specific glycoforms ■ Available in kit form for producing antibodies with G0, G1, G2, or G2S2 glycan profiles (± fucose) ■ Option to remove fucose from the core GlcNAc is also available *For more details, please refer to the PDF document or feel free to contact us.

"GingisKHAN (Kgp)" is a cysteine protease that cleaves human IgG1 at a specific site upstream of the hinge region, producing intact and homogeneous Fab and Fc fragments. This enzyme is used for the characterization of antibody-based biological therapies using LC-MS, Fc glycan analysis, studies on the effects of bispecific antibodies, affinity and binding strength, as well as the identification of post-translational modifications. [Features] - Cleaves human IgG1 at a specific site upstream of the hinge region - Produces intact and homogeneous Fab and Fc fragments - Used for the characterization of antibody-based biological therapies using LC-MS, Fc glycan analysis, studies on the effects of bispecific antibodies, affinity and binding strength, and the identification of post-translational modifications *For more details, please refer to the PDF document or feel free to contact us.

"FabULOUS(SpeB)" is a cysteine protease that digests the hinge regions of various species and subclasses of IgG, generating a pool of uniform Fab fragments. The prepared Fab fragments can be used for affinity studies, Fab glycosylation studies, structural studies, and more. This enzyme requires reducing conditions to show activity against IgG, and under strong reducing conditions, disulfide bonds may be reduced. 【Features】 ■ Digests the hinge regions of various species and subclasses of IgG ■ Generates a pool of uniform Fab fragments ■ The prepared Fab fragments can be used for affinity studies, Fab glycosylation studies, structural studies, and more ■ Cleaves the upper part of the IgG1 hinge region *For more details, please refer to the PDF document or feel free to contact us.

"FabRICATOR Z (IdeZ)" is a cysteine protease that cleaves specific sites downstream of the hinge region of mouse IgG2a and IgG3, generating a homogeneous pool of F(ab')2 and Fc fragments after 2 hours of incubation. Some mouse IgG2a that cannot be digested by FabRICATOR can be easily digested with this product, although a longer incubation time may be required. Additionally, due to the high specificity of the enzyme, there is no risk of over-digestion. 【Features】 ■ Cleaves specific sites downstream of the hinge region of mouse IgG2a and IgG3 ■ Generates a homogeneous pool of F(ab')2 and Fc fragments after 2 hours of incubation ■ Some mouse IgG2a can be easily digested ■ No risk of over-digestion due to the high specificity of the enzyme *For more details, please refer to the PDF document or feel free to contact us.

"FabALACTICA(IgdE)" is a cysteine protease that digests specific sites in the upper hinge region of human IgG1, producing intact and homogeneous Fab and Fc fragments. This enzyme facilitates the characterization of bispecific and multispecific antibodies and the analysis of intact Fc glycosylation. Additionally, it makes research on monovalent binding, higher-order structures, disulfide scrambling, and antibodies with mutations in the hinge region easier. 【Features】 ■ Digests specific sites in the upper hinge region of human IgG1 ■ Produces intact and homogeneous Fab and Fc fragments ■ Facilitates the characterization of bispecific and multispecific antibodies and the analysis of intact Fc glycosylation ■ Makes research on monovalent binding, higher-order structures, disulfide scrambling, and antibodies with mutations in the hinge region easier *For more details, please refer to the PDF document or feel free to contact us.

"FabRICATOR(IdeS)" is an IgG-specific cysteine protease that digests antibodies at a single amino acid site just below the hinge region. It is capable of generating homogeneous F(ab')2 and Fc fragments. This enzyme is widely used for the characterization, quality control, stability testing, production monitoring, and clone selection of antibody-based therapeutics such as mAbs, ADCs (antibody-drug conjugates), biosimilars, and Fc fusion proteins. 【Features】 ■ Digests antibodies at a single amino acid site just below the hinge region ■ Capable of generating homogeneous F(ab')2 and Fc fragments ■ Pre-treatment for LC-MS analysis workflow at an intermediate level - Reduces subunits to generate fragments of 23–25 kDa - Enhances mass resolution to accurately determine important quality attributes *For more details, please refer to the PDF document or feel free to contact us.

"OglyZOR" is an endoglycosidase (O-glycosidase) that specifically hydrolyzes two core 1 O-linked glycans on native glycoproteins. It is used for the removal of O-linked glycans for glycan analysis, confirmation of the presence of O-linked glycans, and reduction of sample heterogeneity. For activity, it is necessary to remove the terminal sialic acid from the O-linked glycans, so SialEXO, a mixture of multiple sialidases, is included, and optimal activity is achieved at pH 6.5 to 7.5 and 37°C. 【Features】 ■ Specifically hydrolyzes two core 1 O-linked glycans on native glycoproteins ■ Used for the removal of O-linked glycans for glycan analysis, confirmation of the presence of O-linked glycans, and reduction of sample heterogeneity ■ Generally does not require denaturation of the substrate ■ Includes SialEXO, a mixture of multiple sialidases ■ Optimal activity is achieved at pH 6.5 to 7.5 and 37°C *For more details, please refer to the PDF document or feel free to contact us.

The enzyme 'SialEXO' is a sialidase useful for the removal and analysis of sialic acid. This enzyme shows activity on both N- and O-linked glycans present in native glycoproteins and free glycans. It is used for the processing of O-glycosylated proteins before digestion with OpeRATOR or OglyZOR, and other applications include reducing sample complexity, charge variant analysis, and exoglycosidase arrays. 【Features】 ■ Useful for the removal and analysis of sialic acid ■ Shows activity on both N- and O-linked glycans present in native glycoproteins and free glycans ■ Used for the processing of O-glycosylated proteins before digestion with OpeRATOR or OglyZOR ■ Hydrolyzes glycoproteins under native conditions, demonstrating high activity over a wide pH range of 6.5 to 9 *For more details, please refer to the PDF document or feel free to contact us.

"GalactEXO" is a mixture of β-galactosidases that hydrolyze terminal galactose linked by β1-3,4 bonds in N- and O-glycosylated proteins. This enzyme is useful for trimming galactose on free glycan structures in exoglycosidase arrays and for producing antibodies with a homogeneous G0/G0F glycan profile. 【Features】 ■ Mixture of β-galactosidases ■ Efficiently promotes the hydrolysis of β1-3,4-linked galactose on glycoproteins ■ Acts on both N- and O-glycosylated structures ■ Completes the hydrolysis of terminal galactose within 2 hours ■ Applicable for glycan sequencing and trimming of glycans in the antibody Fc region *For more details, please refer to the PDF document or feel free to contact us.

The enzyme 'GalNAcEXO' is an α-N-acetylgalactosaminidase that efficiently hydrolyzes terminal GalNAc on glycoproteins. GalNAc, which is bound to serine or threonine and referred to as the Tn antigen, is rapidly and efficiently hydrolyzed by GalNAcEXO, and it also shows activity against α1-3 linked GalNAc. This enzyme serves as a valuable tool in the analysis of complex O-glycosylated proteins that have α-linked GalNAc as a core 1 with a sugar chain that has stopped elongating, reducing sample heterogeneity. 【Features】 ■ Hydrolyzes GalNAc on glycoproteins under native conditions ■ Shows activity in the pH range of 6.0 to 7.6 ■ No cofactors or special buffers are required ■ Reactions on glycoproteins are completed in 2 hours (depending on the nature of the substrate) ■ Complex samples may require overnight incubation *For more details, please refer to the PDF document or feel free to contact us.

"GlycOCATCH" is an affinity resin for the purification of mucin-type O-glycosylated proteins and peptides. This affinity resin is based on an inactive OpeRATOR (enzyme) designed to bind with high affinity to O-glycosylated proteins and peptides. Applications include specific enrichment or removal of O-glycosylated proteins and peptides, glycomics, research on complex samples, and characterization of biopharmaceuticals. 【Features】 - Provided in a spin column format for easy concentration of mucin-type O-glycosylated proteins. - Binds with high affinity due to strong interactions with O-glycosylated proteins, allowing elution using 8M urea. - Elution can also be performed by using the included OpeRATOR (enzyme) to cleave the N-terminal of the O-linked glycan sites of the bound O-glycosylated proteins. *For more details, please refer to the PDF document or feel free to contact us.