ハカレル List of Products and Services

These are two types of products for the relative quantification of dog exosomes containing CD9 and CD63 molecules. - CD9/CD9 Exosome ELISA Kit, Dog - CD63/CD63 Exosome ELISA Kit, Dog Features: - Exosomes contained in blood samples and cell culture supernatants (serum-free) can be directly quantified. - No special equipment is required; measurements can be taken with a standard plate reader. - Stabilized exosomes derived from AZ-ACU (dog urinary epithelial cancer cells) are used as standard reagents. - Relative quantification of each sample is possible by correcting with the exosome standard. - Exosomes are captured using solid-phase dog CD9 antibodies and detected with HRP-labeled dog CD9 antibodies.

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Features of the kit: - It is possible to measure SARS-CoV-2 spike protein in samples such as serum and plasma. - No special equipment is required; measurements can be taken with a standard plate reader. - Using a sandwich ELISA method with two types of antibodies against the RBD of the SARS-CoV-2 S1 domain, it sensitively and specifically measures the SARS-CoV-2 spike protein. - The antibodies used in this ELISA strongly react with all variants of the virus, including the Wuhan strain, as well as Alpha, Beta, Gamma, and Delta variants (manuscript in preparation).

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Feature 1: Spray on the mask! (Spray on the front surface of the mask) Feature 2: Trap viruses (Enhances virus adhesion to the mask) Feature 3: Block infections (Monoclonal antibodies with strong neutralizing activity)

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Our company handles the research reagent 'SARS-CoV-2 Spike Protein RBD Rabbit Fc Tag (100μg)'. This reagent contains the SARS-CoV-2 Spike glycoprotein Receptor-binding domain, with a Rabbit IgG1 Fc tag attached to the C-terminus of Arg319-Phe541, expressed in HEK293 cells and purified using a Protein A column. Please avoid repeated freeze-thaw cycles and store at below -70°C upon receipt. It is also recommended to aliquot during use. 【Product Information】 ■ Protein Structure ■ Purity: >95% (SDS-PAGE) ■ Composition ・SARS-CoV-2 Spike Protein RBD Rabbit Fc Tag 1mg/mL, 0.1M-PBS (pH 7.2–7.4) ■ Storage ・Avoid repeated freeze-thaw cycles ・Store at below -70°C upon receipt ・Aliquot during use is recommended *For more details, please refer to the related link page or feel free to contact us.

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Our company handles the research reagent "Human ACE2 Protein-His Tag (100μg)." This reagent contains a polyhistidine tag attached to the C-terminus of Ser19-Pro738, which corresponds to the extracellular region of human angiotensin-converting enzyme 2, expressed in HEK293 cells and purified using a Ni column. Purity is >95% (SDS-PAGE). It should be stored at -70°C or below upon receipt, and aliquoting is recommended during use. 【Product Information】 ■ Protein Structure ■ Purity: >95% (SDS-PAGE) ■ Composition: Human ACE2 Protein-His Tag 1mg/mL, 0.1M PBS (pH 7.2–7.4) ■ Storage - Avoid repeated freeze-thaw cycles - Store at -70°C or below upon receipt - Aliquoting is recommended during use *For more details, please refer to the related link page or feel free to contact us.

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Components that inhibit the binding of ACE2 and the novel coronavirus may have the potential to become drugs that prevent viral infection. To conduct this screening, we have prepared the following three types of reagents: - Human ACE2 protein - His tag (HAK-ACE2_UL-1) - SARS-CoV-2 spike protein RBD - Rabbit Fc tag (HAK-SPD_UL-1) - Biotinylated SARS-CoV-2 spike protein RBD - Rabbit Fc tag (HAK-SPD_bio-1)

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If this product binds with the novel coronavirus, there is a possibility that the virus's infectivity may weaken. Additionally, if this product is sprayed on infection protection items such as masks, and the novel coronavirus comes into contact with the ACE2 protein applied, it is expected to capture the virus. Since this is a research reagent, it cannot be used for diagnosis or treatment.

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This product is a two-step sandwich ELISA kit that utilizes high-performance monoclonal antibodies against CD9, an exosome marker, and EpCAM, which is said to be a marker for the diagnosis and prognosis of cancer derived from epithelial tissues, to directly and quantitatively detect EpCAM-positive exosomes secreted by cells in human blood and cell culture media.

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This kit allows for the highly sensitive quantification of human PD-L1 in samples using a one-step sandwich method with high-performance antibodies against the immune checkpoint-related molecule PD-L1. Features: - Highly sensitive detection of human PD-L1 using a one-step ELISA method (detection sensitivity: 1 pg/mL) - No special equipment is required; measurements can be taken with a standard plate reader.

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This product is a sandwich ELISA kit that utilizes high-performance antibodies against CD9, an exosome marker, and PD-L1, an immune checkpoint-related molecule, to detect PD-L1 molecules expressed on the surface of exosomes secreted by cells in human blood and cell culture supernatants. PD-L1 is also expressed on exosomes, adopting the same topology as PD-L1 on the surface of cancer cells, with the extracellular domain exposed on the surface of the exosomes. Features: - Highly sensitive detection of human PD-L1 positive exosomes using the ELISA method (detection sensitivity: 0.05 ng/mL) - Direct quantification of PD-L1 positive exosomes contained in human blood samples and cell culture supernatants - No special equipment is required; measurements can be taken with a standard plate reader - Instead of using exosomes themselves, which lack storage stability as standard reagents, stability and reproducibility are ensured by using PD-L1/CD9 fusion protein (standard protein) - Relative quantification of each sample is possible by reading a standard curve using PD-L1/CD9 fusion protein (standard protein)

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Exosomes are gaining attention as targets with new biomarkers, and there is a demand for high-precision analysis of the proteins contained in exosomes. When analyzing blood samples, it is necessary to identify extremely small amounts of target molecules that are only present in exosomes secreted from the disease site, among the overwhelming majority of exosomes derived from normal cells. For this purpose, proteomics analysis methods that allow for the identification of more proteins and quantitative comparisons between samples are the optimal means. In DIA proteome analysis, we conduct proteome analysis using the Data-independent acquisition (DIA) method, which is renowned for its analytical depth and quantitativeness, with the state-of-the-art mass spectrometer Q-Exactive HF-X (Thermo Fisher Scientific). This method allows for the observation of up to 8,000 types of proteins in a single analysis and enables quantitative comparisons of individual proteins between samples. This service is a one-stop service that consistently handles everything from sample preparation to DIA proteome analysis, so customers can obtain high-depth proteome analysis data simply by sending in their samples.

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We offer a step-by-step "antibody production contract service" tailored to your needs. We select antibodies that recognize the three-dimensional structure of target proteins present on the membrane surface using exosomes expressing the target protein, among other methods.

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These are three types of products for the relative quantification of exosomes containing CD9, CD63, or CD81 molecules. - CD9/CD9 Exosome ELISA Kit - CD63/CD63 Exosome ELISA Kit - CD81/CD81 Exosome ELISA Kit Features: - Exosomes contained in blood samples or cell culture supernatants (serum-free) can be directly quantified. - No special equipment is required; measurements can be made with a standard plate reader. - Instead of using exosomes themselves, which lack storage stability as standard reagents, stability and reproducibility are ensured by using standard beads with a particle size of 200 nm that are coated with CD9 or CD63. - Relative quantification of each sample is possible by correcting with CD9, CD63, or CD81 standard beads that mimic exosome structures. - Exosomes are captured using solid-phase CD9, CD63, or CD81 antibodies, and detected with HRP-labeled CD9, CD63, or CD81 antibodies.

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We offer a contract service for the production of exosome detection tools that express and purify proteins in animal cells as an immunogen for antibodies against exosome surface markers. We provide comprehensive support for research on exosome detection methods, focusing on the production of recombinant proteins, the creation of monoclonal antibodies, the establishment of ELISA measurement systems, and exosome ELISA measurements. 【Service Details】 ■ Production of recombinant proteins ■ Creation of monoclonal antibodies against exosome surface markers ■ Establishment of ELISA measurement systems ■ Exosome ELISA measurements *For more details, please download the PDF or feel free to contact us.

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