ニッポンジーン List of Products and Services

The detection of foodborne pathogens is very important in facilities that process wild game or conduct secondary processing. Verotoxin-producing enterohemorrhagic Escherichia coli (EHEC) is one of the representative foodborne pathogens. This product is a primer set for detecting the verotoxin-producing genes VT1 and VT2 of enterohemorrhagic E. coli using the LAMP method. When used in combination with the nucleic acid amplification reagent "GENEMAL LAMP FL Mix" for the LAMP method, the amplified DNA can be detected by a fluorescence detection device*. *The fluorescence detection device for the LAMP method can be "GENEMAL" or a real-time PCR device. (Note) This product is for research purposes only.

• Other Amplification/PCR

The detection of foodborne pathogens is very important in facilities that process wild game or conduct secondary processing. Campylobacter is one of the representative foodborne pathogens. This product is a primer set for detecting Campylobacter jejuni and Campylobacter coli using the LAMP method. When used in combination with the nucleic acid amplification reagent "GENEMAL LAMP FL Mix" for the LAMP method, the amplified DNA can be detected by a fluorescence detection device*. *The fluorescence detection device for the LAMP method can be "GENEMAL" or a real-time PCR device. (Note) This product is for research purposes only.

• Other Amplification/PCR

The detection of foodborne pathogens is very important in facilities that process wild game or conduct secondary processing. Salmonella is one of the representative foodborne pathogens. This product is a primer set for detecting Salmonella using the LAMP method. When used in combination with the nucleic acid amplification reagent "GENEMAL LAMP FL Mix" for the LAMP method, the amplified DNA can be detected by a fluorescence detection device*. *The fluorescence detection device for the LAMP method can be "GENEMAL" or a real-time PCR device. (Note) This product is for research purposes only.

• Other Amplification/PCR

"GENEMAL LAMP FL Mix" is a master mix for isothermal nucleic acid amplification using the LAMP method. It contains heat-resistant strand-displacing DNA polymerase, Mg2+, dNTPs, and optimized buffers necessary for the LAMP method, allowing for DNA amplification simply by adding primers and template nucleic acid to LAMP FL Mix (2×). This product includes an intercalator (a fluorescent dye that binds to double-stranded DNA) and heat-resistant pyrophosphatase, enabling the detection of amplified DNA using a fluorescence detection device*. <Features> ■ High specificity ■ Gene detection possible in just a few minutes ■ Simply add primers and template nucleic acid ■ Optimized for the ultra-lightweight and compact fluorescence detection device "GENEMAL" *Can be used with the fluorescence detection device "GENEMAL" for LAMP method or real-time PCR devices. (Note) Cannot be detected with turbidity measurement devices. This product is a reagent for research purposes. *For more details, please refer to the PDF document or feel free to contact us.

• Other Amplification/PCR

GENEMAL is a compact fluorescence detection device that can perform "isothermal gene amplification" and "fluorescence detection" using the LAMP method in a short time. This device features a heat block and allows for the optimization of reaction conditions by setting measurement parameters on the built-in touch panel. Additionally, it can monitor gene amplification by detecting fluorescence in real-time and can automatically determine results based on pre-set parameters. Furthermore, by combining it with the simultaneously released LAMP primer set for detecting foodborne pathogens and the dedicated amplification reagent (GENEMAL LAMP FL Mix), this device can rapidly detect foodborne pathogens (Salmonella, Campylobacter, and enterohemorrhagic E. coli) in the facility environment. <Features> ■ Compact and ultra-lightweight (weighs 1 kg) ■ Completes nucleic acid amplification and detection in just a few minutes ■ Easy operation via touch panel ■ Automatic determination possible with parameter settings ■ Measurement data can be extracted to a USB memory stick For more details, please refer to the PDF document or feel free to contact us. (Note) This device cannot be used for medical procedures or clinical diagnosis involving human or animal-derived samples. It is intended for research purposes only.

• Other Amplification/PCR

This product is a Cas3 protein derived from Escherichia coli. It is a protein expressed using insect cells infected with a recombinant baculovirus containing the Cas3 gene, and it has undergone affinity purification. It possesses a nuclear localization signal (NLS) and can be utilized for CRISPR-Cas3 genome editing when combined with the Cascade-crRNA complex. 【Features】 - High concentration product at 15 µg/µL - Contains a nuclear localization signal (NLS) - Enables large-scale deletions in the genome when combined with the Cascade-crRNA complex *For more details, please refer to the catalog.

• Other bio-related products

This service provides the design of crRNA (guide RNA) for CRISPR-Cas3 genome editing and the production of a complex of crRNA and Cas proteins (Cas complex for antiviral defense, Cascade-crRNA complex). We co-express five types of proteins that make up the Cascade and one type of crRNA, and deliver the purified Cascade-crRNA complex. Additionally, the proteins that constitute the Cascade (Cas11, Cas7, Cas6) have nuclear localization signals (NLS). By combining the Cascade-crRNA complex produced by this service with the separately sold Cas3 protein NLS that has cleavage activity, it can be utilized in the new genome editing technology "CRISPR-Cas3 system" developed in Japan. *For more details, please refer to the catalog.

• Other bio-related products and services

CRISPR-Cas3 is a genome editing technology developed in Japan based on the research results of Professor Tomoshige Mukaida from the Advanced Animal Genome Research Division at the University of Tokyo Institute of Medical Science, and Professor Junji Takeda from the Osaka University Graduate School of Microbiology. CRISPR-Cas3 has the characteristic of inducing large deletions by continuously cutting the genome, and it also has a very low impact from off-target effects. Recently, Nippon Gene Co., Ltd. has entered into a licensing agreement with C4U Co., Ltd. regarding CRISPR-Cas3 technology and has begun supplying high-purity Cas3 protein and Cascade-crRNA complexes. 【Features of the CRISPR-Cas3 System】 - Large-scale deletions of the genome are possible - Low risk of off-target mutations and high specificity (a highly safe genome editing technology) - Simple licensing makes it easier to establish strategies for the practical application of genome editing technology *For more details, please refer to the catalog.

• Other bio-related products and services

This product is a synthetic RNA that contains a partial sequence of the human RNase P gene and can be used as a positive control RNA for detection systems targeting the human RNase P gene published by the Centers for Disease Control and Prevention (CDC). The detection of the RNase P gene has been selected as an endogenous control to evaluate the quality and operability of nucleic acid extraction from human samples. This product can be used as a positive control for the endogenous control. 【Features】 - Can be used as a positive control RNA for RNase P gene detection systems. For more details, please refer to the PDF document or feel free to contact us. *This product is a reagent for research purposes. It has not been approved or certified as an in vitro diagnostic drug or medical device.

• Real-time PCR

This product is synthetic RNA that contains the full-length sequence of the Nucleocapsid Protein gene (N gene) of the novel coronavirus (SARS-CoV-2) and can be used as positive control RNA for detection systems targeting the N gene. Additionally, since a HindIII site is inserted within the sequence, it is possible to distinguish whether the amplification product originates from this product or from other templates in the event of contamination in the laboratory. 【Features】 - Can be used as positive control RNA for N gene detection systems - PCR products amplified from positive control RNA can be distinguished by restriction enzyme treatment For more details, please refer to the PDF document or feel free to contact us. *This product is a research reagent. It has not been approved or certified as an in vitro diagnostic drug or medical device.

• Real-time PCR

This is a master mix for real-time PCR (2× concentration). With chemically modified hot-start DNA polymerase and optimized buffer, it suppresses non-specific amplification and allows for accurate analysis across a wide range of template concentrations. <Features> ○ Usable for SNP genotyping experiments ○ Capable of carryover prevention treatment with UNG (*) ○ Compatible with various plate types of real-time PCR devices (*) This product does not contain UNG. *For more details, please refer to the PDF document or feel free to contact us.

• Real-time PCR

This is a master mix for real-time PCR (2× concentration). It features a chemically modified hot-start DNA polymerase and optimized buffer to suppress non-specific amplification, achieving high specificity and reproducibility. Additionally, by adding the separately sold Uracil-N-Glycosylase (UNG), carryover prevention treatment can be performed. <Features> ◎ Outstanding cost performance ◎ High specificity and amplification efficiency ◎ Carryover prevention with the addition of UNG (*) ◎ Compatible with various real-time PCR devices (pre-adjusted with dye for calibration) * This product does not contain Uracil-DNA Glycosylase (UNG). * For more details, please refer to the PDF document or feel free to contact us.

• Real-time PCR

Nippon Gene Co., Ltd. provides products tailored to customer needs, ranging from "small-scale production in the lab to bulk production through scale-up to raw material supply" as a manufacturer of life science-related reagents. <Features> ■ A commitment to "manufacturing with biotechnology" and a thorough dedication to quality since our establishment: We offer flexible one-stop support from raw material procurement, manufacturing, inspection, to shipping. ■ Contract manufacturing of reagents related to labs and production (OEM and raw material supply, etc.): We accommodate small lots from product prototyping to mass production. ■ Manufacturing and quality testing can be conducted in environments suited to specific applications: We provide products with the necessary quality, such as manufacturing in clean rooms and conducting DNase/RNase tests. ■ Responding to customer requests with a high level of technical expertise: We manufacture using various experiences and know-how cultivated over more than 40 years. Our manufacturing is conducted under a rigorous quality management system certified by ISO9001 or ISO13485. ★ Currently, we are offering a collection of case studies showcasing various examples of our contract manufacturing services. If you are interested, please check it out from "PDF Download."

• Other bioproduct raw materials

This product is an enzyme that possesses 5'→3' DNA polymerase activity and strand displacement activity, capable of synthesizing new DNA strands while dissociating the hydrogen bonds of the double-stranded DNA template. The heat-stable strand-displacing DNA polymerase does not require the dissociation of double-stranded DNA due to its characteristics, allowing DNA synthesis at a constant temperature and making it resistant to synthesis inhibition caused by the secondary structure of DNA. **Features** - Has 5'→3' DNA polymerase activity and strand displacement activity - Enables DNA synthesis at a constant temperature - Suitable for synthesizing DNA strands with high GC content - Not affected by inhibition due to DNA secondary structures **Optimal Reaction Temperature** 50°C to 55°C **Inactivation Time** *1 70°C for 5 minutes *1 Inactivation temperature when the enzyme stock solution is directly heat-denatured **Applications** Applications utilizing strand displacement activity (such as isothermal gene amplification methods)

• Other Amplification/PCR

This product is an enzyme that possesses 5’→ 3’ DNA polymerase activity and strand displacement activity, capable of synthesizing new DNA strands while dissociating the hydrogen bonds of the double-stranded DNA template. The heat-stable strand displacement type DNA polymerase does not require the dissociation of double-stranded DNA due to its characteristics, allowing DNA synthesis at a constant temperature, and it is not affected by synthesis inhibition caused by the secondary structure of DNA. 【Features】 ■ Has 5’→ 3’ DNA polymerase activity and strand displacement activity ■ Capable of DNA synthesis at a constant temperature ■ Suitable for synthesizing DNA strands with high GC content ■ Not inhibited by the secondary structure of DNA 【Optimal Reaction Temperature】 60°C to 70°C 【Inactivation Time】*1 85°C for 5 minutes *1 Inactivation temperature when the enzyme stock solution is directly heat-denatured 【Applications】 Applications utilizing strand displacement activity (such as isothermal gene amplification methods)

• Other Amplification/PCR

This product is an enzyme that has 5'→3' DNA polymerase activity and strand displacement activity, capable of synthesizing new DNA strands while dissociating the hydrogen bonds of the double-stranded DNA template. The heat-stable strand-displacing DNA polymerase does not require the dissociation of double-stranded DNA due to its characteristics, allowing DNA synthesis at a constant temperature, and it is not affected by synthesis inhibition caused by the secondary structure of DNA. 【Features】 ■ Has 5'→3' DNA polymerase activity and strand displacement activity ■ Capable of DNA synthesis at a constant temperature ■ Suitable for synthesizing DNA strands with high GC content ■ Not inhibited by the secondary structure of DNA 【Optimal Reaction Temperature】 60°C to 65°C 【Inactivation Time】*1 80°C for 5 minutes *1 Inactivation temperature when the enzyme stock solution is directly heat-denatured 【Applications】 Applications utilizing strand displacement activity (such as isothermal gene amplification methods)

• Other Amplification/PCR

Human rhinovirus type 14-derived 3C protease (HRV-3C Protease) recognizes the specific 8 amino acid sequence Leu-Glu-Val-Leu-Phe-Gln-Gly-Pro and cleaves between Gln and Gly. This product is a high-purity protein fused with a GST tag at the N-terminus and an 8×His tag at the C-terminus. 【Features】 - Cleaves tags from target proteins with the recognition sequence - After the reaction, removal of this enzyme is easy using the GST tag - Minimal buffer composition without additives such as surfactants - Ideal for sample preparation in protein science research

• Other Protein Research

Tobacco Etch Virus-derived protease (TEV Protease) recognizes a specific 7-amino acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-Gly/Ser and cleaves between Gln and Gly (or between Gln and Ser). This product does not contain glycerol. Additionally, due to its minimal buffer composition that does not include additives such as surfactants, it is suitable for the preparation of samples used in protein science research, such as protein crystallography. 【Features】 - Cleaves tags from target proteins containing the TEV protease recognition sequence - Affinity purification tags (8×His, 6×HN) are added, allowing for easy removal of the enzyme after the reaction - Glycerol-free - Minimal buffer composition without additives such as surfactants - Ideal for sample preparation in protein science research

• Other Protein Research

This product is a kit for subjects to collect their own saliva samples for PCR testing. It allows for easy placement of the saliva sample into a tube provided by the testing organization. After collecting the saliva sample, the used saliva collection container and dropper can be conveniently returned to the zip bag for disposal, ensuring hygiene. Features: - Anyone can reliably collect saliva - Safe design prevents secondary infections - Comes in a zip bag for easy disposal *For more details, please refer to the PDF materials or feel free to contact us.

• Bottles and containers

This is a kit for extracting and purifying viral RNA from body fluids such as sputum, nasopharyngeal swabs, saliva, and serum using a spin column. It utilizes the principle of RNA adsorption to silica in the presence of chaotropic ions, without the use of toxic organic solvents like phenol or chloroform. The extraction solution, optimized for viral lysis and the degradation of contaminating proteins, along with Proteinase K, allows for the easy acquisition of high-purity RNA in about 30 minutes. Features: - Viral RNA can be purified in about 30 minutes - High operability with spin columns - High sensitivity detection possible with methods such as PCR *For more details, please refer to the PDF document or feel free to contact us.

• RNA Purification Kit

At Nippon Gene, we manufacture and develop reagents for genetic engineering research. Utilizing the manufacturing know-how we have cultivated so far, we prepare and aliquot reagents and enzymes that make up the kit, and manufacture original kits. 【Contracted Services】 ■ Manufacturing and aliquoting of components ■ Various quality tests ■ Creation of accompanying documents, labels, and kit boxes ■ Kit assembly ■ Provision of documents such as test reports *For more details, please refer to the PDF materials or feel free to contact us.

• OEM

"Pho DNA Polymerase" is a heat-stable DNA polymerase derived from the hyperthermophilic archaeon Pyrococcus horikoshii OT3. It possesses 3’→ 5’ exonuclease activity (proofreading activity), so the resulting PCR products have blunt ends. If necessary, they can be ligated to blunt-ended vectors after phosphorylation. Additionally, the mutation frequency is approximately 10 times lower than that of Pol I type, and due to its high fidelity of synthesis, it is suitable for PCR where accurate amplification is required. 【Features】 ■ Has 3’→ 5’ exonuclease activity (proofreading activity), resulting in blunt-ended PCR products ■ High fidelity of synthesis allows for accurate PCR ■ Capable of amplifying long-chain DNA and DNA with high GC content ■ Less affected by PCR inhibitors such as SDS ■ No enzyme inactivation observed at PCR denaturation temperatures of 92℃ to 98℃ *For more details, please refer to the related links or feel free to contact us.

• reagent
• enzyme
• Other research reagents

The "Go-to DNA Polymerase" we handle is a PCR enzyme that combines α-type DNA polymerase derived from Pyrococcus sp. with a modified elongation enhancer. It possesses 3’→ 5’ exonuclease activity, which allows it to remove incorrectly incorporated nucleotides during the polymerase reaction, making it suitable for applications requiring high accuracy in DNA fragments, such as cloning. Additionally, the modified elongation enhancer improves the shortcomings of α-type DNA polymerase, such as low elongation speed and amplification efficiency. 【Features】 ■ Mixture of α-type DNA polymerase and modified elongation enhancer ■ Faster elongation speed and better DNA amplification efficiency due to the modified elongation enhancer ■ Has proofreading activity, ensuring high accuracy *For more details, please refer to the related links or feel free to contact us.

• reagent
• enzyme
• Other research reagents

The "Hot-Start Gene Taq" we handle is a heat-resistant DNA polymerase for hot start PCR, which undergoes enzyme activation treatment at 95°C for 5 minutes before entering the PCR cycle. It is a modified Taq DNA polymerase called "Gene Taq FP," which minimizes contamination from host bacterial DNA and has been chemically modified. The resulting PCR products can be used for TA cloning. Additionally, this product comes with two types of reaction buffers: the conventional 10 x Gene Taq Universal Buffer and the 10 x Brilliant Buffer. 【Features】 ■ High specificity and high DNA yield ■ Hot-Start function allows for reaction mixture preparation at room temperature ■ Suitable for multiplex PCR ■ Comes with two types of buffers that can be used according to experimental conditions *For more details, please refer to the related links or feel free to contact us.

• reagent
• enzyme
• Other research reagents

"Hot-Start Gene Taq NT" is a heat-resistant DNA polymerase for hot-start PCR, which undergoes an activation process at 95°C for 5 minutes before entering the PCR cycle. It has the same functionality as natural Taq DNA polymerase, with chemical modifications applied to "Gene Taq NT," possessing 5'→ 3' exonuclease activity but lacking 3'→ 5' exonuclease activity. Additionally, it has terminal transferase activity, allowing the resulting PCR products to be used for TA cloning. 【Features】 ■ High specificity ■ Hot-Start functionality ■ PCR products can be used for TA cloning *For more details, please refer to the related links or feel free to contact us.

• reagent
• enzyme
• Other research reagents

The "Hot-Start Gene RED PCR Mix" is a 2x premix type PCR reagent that has been hot-started using an antibody method, based on the conventional Gene RED PCR Mix Plus. The buffer composition has been optimized to support high-speed PCR (extension time of 10 seconds/kb). It can amplify relatively GC-rich sequences (about 69%) and long fragments (10kb). Additionally, since the dye and density adjustment agents are premixed, the reaction solution after PCR can be directly applied to an agarose gel. 【Features】 ■ Simple and speedy usage ■ Suppresses non-specific amplification through the Hot Start PCR method ■ Accommodates a wide range of experiments while ensuring sufficient amplification ■ PCR can be performed by simply adding template DNA and primers *For more details, please refer to the related links or feel free to contact us.

• reagent
• enzyme
• Other research reagents

"Gene RED PCR Mix Plus" is a 2x premix type PCR reagent. It contains Taq DNA Polymerase, dNTPs, Mg2+, and other components necessary for PCR, allowing you to perform PCR by simply adding template DNA and primers. Additionally, the buffer composition has been optimized to accommodate fast PCR, making it possible to amplify relatively GC-rich sequences and long fragments (up to 10kb). The resulting PCR products can be used for TA cloning, making it suitable for a wide range of experiments. 【Features】 ■ Easy-to-use 2x premix type ■ Capable of fast reactions (extension time of 10 seconds/kb) ■ Contains two colors of dye, ready to apply as is ■ Compatible with various samples *For more details, please refer to the related links or feel free to contact us.

• reagent
• enzyme
• Other research reagents

The "Gene Taq FP" that we handle is a Taq DNA polymerase that has been purified to a higher degree using our unique technique, minimizing contamination from host bacterial genomic DNA. This product has the same functionality as Gene Taq, with a high amplification efficiency for DNA fragments of 1 kbp or less, and it features the absence of 5'→3' exonuclease activity. Additionally, it possesses terminal transferase activity, allowing the obtained PCR products to be used for TA cloning. 【Features】 ■ Further purified Gene Taq ■ Taq DNA polymerase with minimal contamination from host bacterial DNA ■ PCR products can be used for TA cloning *For more details, please refer to the related links or feel free to contact us.

• reagent
• enzyme
• Other research reagents

"Gene Taq NT" is a heat-stable DNA polymerase that is cloned from the DNA polymerase gene of Thermus aquaticus YT1, expressed in E. coli, and isolated and purified. It has the same function as the natural Taq DNA polymerase and can be used for standard PCR. Additionally, it possesses terminal transferase activity, allowing the resulting PCR products to be used for TA cloning. 【Features】 ■ Has the same function as natural Taq DNA polymerase ■ PCR products can be used for TA cloning ■ Possesses terminal transferase activity *For more details, please refer to the related links or feel free to contact us.

• reagent
• enzyme
• Other research reagents

"Gene Taq" is a heat-stable DNA polymerase that has been modified from the DNA polymerase gene of Thermus aquaticus YT1, expressed in and purified from E. coli, making it suitable for PCR methods. It possesses terminal transferase activity, allowing the resulting PCR products to be used for TA cloning. Since part of the N-terminal of Taq DNA polymerase has been deleted, it does not have 5' → 3' exonuclease activity. Additionally, it shows particularly high amplification efficiency for DNA fragments under 1 kbp. 【Features】 ■ Particularly high amplification efficiency for DNA fragments under 1 kbp ■ PCR products can be used for TA cloning ■ Part of the N-terminal of Taq DNA polymerase has been deleted *For more details, please refer to the related links or feel free to contact us.

• reagent
• enzyme
• Other research reagents

At Nippon Gene, we offer a contract preparation service for manufacturing solutions tailored to your specified composition, standards, and volume. We can also accommodate manufacturing in desired environments (such as clean rooms) and conduct quality inspections in accordance with pharmacopoeia standards. We solve concerns such as "I need a quality-assured preparation," "It's a hassle to prepare reagents for routine work every time," and "It's difficult to prepare solutions containing toxic organic solvents in-house." 【Features】 ■ Supply products that pass necessary quality tests and meet standards ■ Support from small scale to large scale ■ Capable of conducting nuclease tests and sterility tests ■ Manufacturing of solutions for various applications and OEM supply is also possible 【Service Flow】 1. Inquiry ➡ 2. Quotation ➡ 3. Order ➡ 4. Manufacturing and Inspection ➡ 5. Delivery *For more details, please refer to the PDF document or feel free to contact us.

• Other Contract Life Science Specialization

The "LF-8 Plus" is a device equipped with two types of measuring instruments: turbidity and fluorescence, allowing both data to be obtained in a single measurement. In addition to turbidity data for gene detection using the LAMP method, it can be combined with intercalating dyes or fluorescent probes (such as Quenching Probes and Molecular Beacons). The included PC software "LF-8 Manager" enables turbidity measurement and gene polymorphism analysis, while the PC software "LF-8 Analyzer" allows for the analysis of gene amplification data using fluorescent substances and melting curve analysis, making it possible to perform various measurements using the LAMP method, not just gene polymorphism analysis. 【Features】 ■ Equipped with two types of measuring instruments (turbidity and fluorescence) ■ Analysis time of just a few minutes ■ Very simple operation ■ Capable of gene polymorphism analysis *For more details, please refer to the PDF document or feel free to contact us.

• Other measurement and measuring equipment

The "10 x RE Buffer Set" allows you to choose 6 out of the 10 types of 10× buffers included with our restriction enzymes. We offer a lineup that includes 10×L Buffer, 10×M Buffer, 10×H Buffer, and more, and you can also select the same buffer in bulk. Nippon Gene ships this product at room temperature, and by storing it in the refrigerator or freezer upon arrival, you can use it without any issues. 【Partial Lineup】 ■10×L Buffer ■10×M Buffer ■10×H Buffer ■10×A Buffer ■10×B Buffer *For more details, please refer to the related links or feel free to contact us.

• reagent

At Nippon Gene, we offer custom buffer preparation tailored to your specifications. Examples include 0.5M EGTA, guanidine thiocyanate solution, and 10 x TBE (for sequencers). Please fill out the request form with your desired composition, volume, grade, and preparation conditions, and send it to us. We will provide you with a quote for price and delivery time. [Contents (partial)] ■ Price: Inquiry ■ Delivery time: Inquiry ■ Preparation examples ・ 0.5M EGTA ・ Guanidine thiocyanate solution ・ 10 x TBE (for sequencers), etc.

• Other Contract Life Science Specialization

"CLEAR STAIN Blue" is a reagent for staining nucleic acids after agarose gel electrophoresis. This product stains nucleic acids blue for visualization, eliminating the need for detection equipment. Additionally, it has no mutagenicity like ethidium bromide (EtBr), making it very safe and easy to handle. 【Features】 ■ Detection sensitivity is comparable to EtBr ■ Non-mutagenic and very safe ■ No specialized detection equipment required ■ Visible under natural light ■ Can be excised from the gel *For more details, please refer to the related links or feel free to contact us.

• reagent

We would like to introduce our nucleic acid staining reagent, 'EtBr Solution'. This product is made by dissolving 10mg of ethidium bromide in 1ml of purified sterilized water. There is no need for the precise weighing or dissolving of hazardous ethidium bromide powder, making it convenient and safe. 【Specifications (partial)】 ■ Appearance: Red solution ■ Concentration: 10mg/ml ■ Measurement wavelength ・λ ex = 254nm (second peak) or 518nm ・λ em = 605nm *For more details, please refer to the related links or feel free to contact us.

• reagent

"2 X DGGE Loading Buffer" is a product for DGGE (Denaturing Gradient Gel Electrophoresis). Use half the amount relative to the sample volume. It contains Bromophenol Blue and Glycerol. As an example of use, add an equal amount of this product to the DNA sample solution, mix by pipetting several times, and then gently apply to the wells of the denaturing gradient gel for electrophoresis. 【Features】 ■ For DGGE ■ Use half the amount relative to the sample volume ■ Contains Bromophenol Blue and Glycerol *For more details, please refer to the related links or feel free to contact us.

• reagent

"6 x Loading Buffer Orange G" is a reagent added when preparing nucleic acid samples for electrophoresis. Use 1/6 of the amount relative to the sample volume. Ficoll PM400 is used as the density additive, which reduces the occurrence of band smiling compared to glycerol. Additionally, by not using BPB, it is possible to take UV photographs of the gel's central area without any dye shadow during photography. 【Features】 ■ Added when preparing nucleic acid samples for electrophoresis ■ BPB is not used ■ Almost the leading edge of the migrating substance can be observed ■ Ficoll PM400 is used as the density additive *For more details, please refer to the PDF document or feel free to contact us.

• reagent

"6 X Loading Buffer Double Dye" is a reagent added when preparing nucleic acid samples for electrophoresis. By not using BPB, it is possible to take UV photographs without any dye shadows in the center of the gel. As an example of use, add 2μl of this product to a 10μl DNA sample solution, mix by pipetting several times, and then gently inject (apply) 10μl of the mixture into the wells of an agarose gel to perform electrophoresis. 【Features】 - Added when preparing nucleic acid samples for electrophoresis - Used at 1/6 the amount relative to the sample volume - BPB-free - Less prone to band smiling compared to glycerol *For more details, please refer to the related links or feel free to contact us.

• reagent

"6 X Loading Buffer Triple Dye" is a reagent added when preparing nucleic acid samples for electrophoresis. It is used by adding 1/6 of the amount relative to the sample volume. The dyes used include Orange G, which allows for the visualization of the nearly leading edge of the electrophoresed sample, Bromophenol Blue (BPB), and Xylene Cyanol FF (XC), which helps confirm the mobility of high molecular weight substances. Ficoll PM400 is used as a density additive, which reduces the occurrence of band smearing compared to glycerol. 【Features】 - Added when preparing nucleic acid samples for electrophoresis - Used by adding 1/6 of the amount relative to the sample volume - Ficoll PM400 is used as a density additive - Reduced band smearing compared to glycerol *For more details, please refer to the related links or feel free to contact us.

• reagent

"Loading Buffer" is a reagent added when preparing nucleic acid samples for electrophoresis. It is used by adding 1/5 to 1/10 of the sample volume. It contains two types of dyes (Bromophenol Blue and Xylene Cyanol) and Glycerol. Since this product contains SDS, it may affect the electrophoresis patterns of DNA and bound proteins (such as Taq MutS). On the other hand, if you want to terminate an enzyme reaction or if the sample solution contains a large amount of protein, having SDS included will result in less impact on the electrophoretic mobility. 【Features】 ■ Added when preparing nucleic acid samples for electrophoresis ■ Used by adding 1/5 to 1/10 of the sample volume ■ Contains two types of dyes and Glycerol ■ Affects the electrophoresis patterns of DNA and bound proteins *For more details, please refer to the related links or feel free to contact us.

• reagent

The "SDS-PAGE 10 x Running Buffer" is a stock solution at a concentration 10 times that of the usage conditions. It is a buffer solution composed of Tris, glycine, and sodium dodecyl sulfate, available in two sizes: 1L and 5L. When using it, dilute with water to create "SDS-PAGE 1 x Running Buffer." 【Specifications】 ■Composition: 250 mmol/l Tris, 1,920 mmol/l Glycine, 1% (w/v) SDS ■Working Solution ・(1 x Buffer Composition) 25 mmol/l Tris, 192 mmol/l Glycine, 0.1% (w/v) SDS ■Storage: Room temperature ■Notes: Confirmed that SDS polyacrylamide gel electrophoresis can be performed without issues. *For more details, please refer to the related links or feel free to contact us.

• reagent

The "5 x TBE" is a stock solution at a concentration five times the usage conditions. It is a buffer composed of Tris, boric acid, and EDTA, commonly used during electrophoresis, similar to TAE (10 x TAE, 50 x TAE). When used in agarose gel electrophoresis, it can be diluted to "0.5 x TBE" with water at a 10-fold dilution. For polyacrylamide gel electrophoresis, "1 x TBE" is used, diluted 5 times with water. 【Specifications】 ■ Composition: 0.445 mol/l Tris-borate, 10 mmol/l EDTA ■ Dilution Guidelines - Agarose Gel Electrophoresis: 10-fold dilution (Working Solution: 0.5 x TBE) - Polyacrylamide Gel Electrophoresis: 5-fold dilution (Working Solution: 1 x TBE) ■ Storage Method: Room temperature ■ Notes: Confirm that normal electrophoresis can be performed without issues. *For more details, please refer to the related links or feel free to contact us.

• reagent

"50 x TAE" is a stock solution that is at a concentration 50 times that of the usage conditions. It is very convenient as it does not take up space during storage and can be used simply by diluting it 50 times with water. It is a buffer composed of Tris, acetate, and EDTA, and is commonly used during electrophoresis, similar to TBE Buffer. When using it, a 1 x TAE (working solution) diluted 50 times with water is utilized. 【Features】 ■ Can be used simply by diluting 50 times with water ■ Composition: 2 mol/l Tris-acetate, 50 mmol/l EDTA ■ Working Solution: (1 x TAE composition) 40 mmol/l Tris-acetate, 1 mmol/l EDTA ■ Confirmed that normal electrophoresis can be performed without issues *For more details, please refer to the related links or feel free to contact us.

• Other research reagents

"10 x TAE" is a stock solution that is at a concentration 10 times the usage condition. It is very convenient because it does not take up space during storage and can be used simply by diluting it 10 times with water. It is a buffer composed of Tris, acetate, and EDTA, commonly used during electrophoresis, similar to TBE Buffer. When using it, a 1 x TAE (working solution) diluted 10 times with water is employed. 【Features】 ■ Does not take up space during storage and can be used simply by diluting it 10 times with water ■ Composition: 0.4 mol/l Tris-acetate, 10 mmol/l EDTA ■ Working Solution: (1 x TAE composition) 40 mmol/l Tris-acetate, 1 mmol/l EDTA ■ Confirmed that normal electrophoresis can be performed without issues *For more details, please refer to the related links or feel free to contact us.

• Other research reagents