ADSTEC Corporation List of Products and Services

The RNASep series from ADS Biotec in the United States is capable of handling everything from mRNA analysis to purification.

• High Performance Liquid Chromatograph

The organoid culture kits manufactured by ADS Biotec in the United States can be used for organoid research. You can choose the kit that suits you from five different types. 1. Human colon organoid 2. Human fallopian tube organoid 3. Mouse colon organoid 4. Mouse fallopian tube organoid 5. Mouse liver organoid

• Culture medium

By using the RNASep series HPLC columns from ADS Biotec in the United States and TEAA buffer, you can analyze and purify mRNA. The column beads are made of alkylated polystyrene-divinylbenzene, allowing for repeated use by cleaning the column. They are compatible with many HPLC systems. A key strength is the ability to analyze and purify long-chain mRNA up to 6K. Customers around the world utilize this product, and it boasts a significant number of publications.

• High Performance Liquid Chromatograph

The DNASep series from ADS Biotec is an HPLC column for DNA analysis.

• High Performance Liquid Chromatograph

The HPLC columns and buffers for oligonucleotide analysis manufactured by ADS Biotec in the United States use beads made of alkylated polystyrene-divinylbenzene. The columns are referred to as the OligoSep series. Oligonucleotides are used in research for antisense and siRNA pharmaceuticals.

• High Performance Liquid Chromatograph

The HANABI series of chromosome analysis preprocessing devices from AD Estec Co., Ltd. automates the preprocessing steps in chromosome analysis, contributing to increased productivity in the inspection process and the prevention of human errors. Harvester: Automation of the cell harvesting process Spreader: Automation of the spreading process Stainer: Automation of the staining process We provide application notes (free) summarizing cases where the HANABI series has been used for chromosome analysis. If you are interested, we would appreciate it if you could contact us using the inquiry form below. Thank you very much.

• Other bio-related products and services

The HANABI Avatar Oviduct Organoid Growth Media Kit – Human is a kit for culturing human oviduct organoids manufactured by ADS Biotec. It can be used for both normal and diseased tissues.

• Culture medium

The HANABI Avatar Oviduct Organoid Growth Media Kit – Murine is a kit for culturing mouse oviduct organoids manufactured by ADS Biotec. It can be used for both normal and diseased tissues.

• Culture medium

The HANABI Avatar Colon Organoid Growth Media Kit – Human is a kit for culturing human colon organoids manufactured by ADS Biotec. It can be used for both normal and diseased tissues.

• Culture medium

The HANABI Avatar Liver Organoid Growth Media Kit – Murine is a kit for the culture of mouse liver organoids. It can be used for both normal and diseased tissues.

• Culture medium

The HANABI Avatar Colon Organoid Growth Media Kit – Murine is a kit for culturing mouse colon organoids manufactured by ADS Biotec. It can be used for both normal and diseased tissues.

• Culture medium

The buffer of hexylamine acetate (HAA) manufactured by ADS Biotec (formerly Transgenomic) can be used to create a mobile phase for nucleic acid analysis using ion-pair reverse phase HPLC. HAA is suitable for samples with a high GC content or for separation at the single nucleotide level. The component is 2.0M HAA. By using this product, you can create a buffer of HAA suitable for columns made by ADS Biotec. It is sold in cases containing six 200mL bottles. One case can produce 24L of HAA at a concentration of 0.1M.

• reagent

ADS Biotec in the United States also offers custom buffer solutions tailored to meet customer needs. ■ About ADS Biotec ADS Biotec (formerly Transgenomic) has a manufacturing facility for reagents located in Omaha, Nebraska, and has been manufacturing and selling HPLC buffers for nucleic acid analysis for over 20 years. In addition to HPLC buffers, we have a track record of producing custom buffers for our customers. ■ Sales Units We can accommodate orders from several liters to several hundred liters. For orders of 40 liters or more, we sell in bulk sizes. By using ADS Biotec's large-capacity buffers, it is possible to maintain consistent quality from the research stage. ■ Benefits The buffers are pre-adjusted for concentration, so you can use them immediately upon receipt, reducing the time and cost associated with buffer preparation. ■ Quality Control ADS Biotec is ISO13485 certified and complies with cGMP, ensuring thorough quality control in our manufacturing and sales processes.

• Buffer

The Triethylammonium Acetate Solution (TEAA) 2.0 M manufactured by ADS Biotec (formerly Transgenomic) from USA is a product for creating mobile phases for nucleic acid analysis using ion-pair reverse-phase High Performance liquid chromatography (HPLC). The component is 2M triethylammonium acetate (TEAA). It can be particularly used for preparing TEAA Buffer A and Buffer B suitable for columns made by ADS Biotec. TEAA Buffer A: 0.1M TEAA TEAA Buffer B: 0.1M TEAA, 25% acetonitrile It is sold in cases containing six 200mL bottles. One case can produce 24L of 0.1M concentration TEAA. It can also be used for the separation of double-stranded and denatured nucleic acids by HPLC. ADS Biotec has obtained ISO 9001 and ISO 13485 certifications and conducts thorough quality control.

• reagent

The OligoSep Prep HC Cartridge manufactured by ADS Biotec (formerly Transgenomic) is a column for the purification of oligonucleotides using ion-pair reverse phase HPLC. It uses porous beads made of alkylated polystyrene-divinylbenzene (PS-DVB). Due to the porous nature of the beads, the surface area is increased, allowing for the purification of larger amounts of samples. It can be used for the purification of synthetic oligos. It is compatible with various types of high-performance liquid chromatography (HPLC) systems. By using ADS Biotec's buffer as the mobile phase, high-precision separation can be achieved. Additionally, by following the procedures outlined in the instruction manual and washing the column with 75% acetonitrile, the column can be reused multiple times.

• High Performance Liquid Chromatograph

The OligoSep HC Cartridge manufactured by ADS Biotec (formerly Transgenomic) is a column that uses porous beads made of alkylated polystyrene-divinylbenzene. Due to the porous nature of the beads, the surface area of the column beads is larger compared to non-porous beads, allowing for the purification of a greater volume of samples. It can be used not only for the purification of oligonucleotides but also for the analysis of single-stranded and double-stranded DNA. It is compatible with various types of high-performance liquid chromatography (HPLC) systems. By using buffers manufactured by ADS Biotec as the mobile phase, high-precision separations can be achieved. Additionally, by following the procedures outlined in the instruction manual and washing the column with 75% acetonitrile, the column can be reused multiple times.

• High Performance Liquid Chromatograph

The OligoSep Cartridge manufactured by ADS Biotec (formerly Transgenomic) is a column for oligonucleotide analysis using ion-pair reverse phase HPLC. The packing material consists of non-porous beads made of alkylated polystyrene-divinylbenzene. Due to the non-porous nature of the beads, it allows for higher precision purification. It can be used not only for quality control of oligonucleotides but also for high-precision analysis of both single-stranded and double-stranded DNA. It is compatible with various types of high-performance liquid chromatography (HPLC) systems. By using buffers manufactured by ADS Biotec as the mobile phase, high-precision separation can be achieved. Additionally, by following the procedures outlined in the instruction manual to wash the column with 75% acetonitrile, the column can be reused multiple times.

• High Performance Liquid Chromatograph

HANABI P7 is a device that automates the unfolding process in chromosome analysis pre-treatment. To unfold chromosomes, it is important to control various conditions such as humidity, temperature, and airflow. The HANABI P7 is designed with our proprietary technology to strictly control the environment for temperature, humidity, and airflow, allowing for consistent unfolding results unaffected by weather or seasonal changes. Once the spreading condition is ready, the instrument can spread the samples onto the slides automatically. - Up to 24 samples can be processed in a single run. A maximum of 72 slides can be processed in under one hour. - Excessive dispersion and overlap of chromosomes are minimized, resulting in an increased yield of analyzable metaphases compared to traditional methods. - It is necessary to prevent cross-contamination when processing chromosome samples. The HANABI P7 comes standard with a code reader, enabling the linking of sample information. - A HEPA filter maintains a clean environment within the device. - Up to six protocols can be registered. If the same protocol as the previous one is used, there is no need to register it again, allowing for smooth processing.

• Other bio-related products

The HANABI-SA5000 is a pre-treatment aging device for dyeing. It can accommodate up to 5 slides. The operation is simple: just set the aging time on the timer and press start. The amount of aging can be adjusted based on the time setting. Its compact size makes it easy to transport, allowing you to make effective use of your workspace. By using UV aging for the aging process, which is the most time-consuming step in dyeing, the time is reduced to about 30 seconds, enabling quicker inspection results.

• Other cell research

This is a column for high-performance liquid chromatography (HPLC) that is effective for the research and development of mRNA pharmaceuticals and mRNA vaccines produced by the U.S. company ADS Biotec. It is a large-capacity purification column that can be used to purify up to 2 mg of mRNA.

• Other purification and extraction

The RNASep SemiPrep column manufactured by ADS Biotec (formerly Transgenomic) in the United States is a column for RNA purification using high-performance liquid chromatography (HPLC) that can also be used for the research and development of mRNA drugs and mRNA vaccines. The column beads are made from polymer beads, allowing for use over a wider pH range compared to conventional silica bead columns. It is utilized by customers worldwide, primarily in Europe and the United States.

• Other purification and extraction

Mobile phase for nucleic acid analysis that can also be used in the research and development of mRNA drugs and mRNA vaccines.

• Other purification and extraction

TEAA Buffer A is an aqueous solution of 0.1M triethylammonium acetate (TEAA). When used in conjunction with TEAA Buffer B in a gradient, it enables the analysis and purification of mRNA and nucleic acids.

• Other purification and extraction

The RNASep Prep column manufactured by ADS Biotec (formerly Transgenomic) in the United States is a column for RNA analysis using high-performance liquid chromatography (HPLC) that can also be used for the research and development of mRNA drugs and mRNA vaccines. The column beads are made from polymer beads, allowing for use over a wider pH range compared to columns with conventional silica beads. It is utilized by customers worldwide, primarily in Europe and the United States.

• Other purification and extraction

The "HANABI P-1000" is a cell harvesting device for culture that automatically performs reagent injection and suction on samples loaded in dishes. It is designed to thoroughly pursue improvements in processing speed, efficiency, and quality maintenance in cell harvesting. By placing culture dishes on the built-in five-tier disk, the circulation of reagents is carried out by each injection and suction probe, enabling parallel operation of samples and significantly reducing processing time. 【Features】 ■ Two models available (for a maximum of 40 samples, for a maximum of 65 samples) ■ Up to 8 protocols can be set ■ Easy operation with a handy controller ■ Can automatically perform high-quality cell harvesting processing for dish samples *For more details, please refer to the PDF document or feel free to contact us.

• Other bio-related products

The "HANABI P5" is a compact automatic device for preparing chromosome metaphase specimens, suitable for genetic analysis such as FISH, CGH, and G-banding. It automates the unfolding process in chromosome analysis pre-treatment. It can automatically process up to 10 samples and 20 slides, reducing the burden on operators. Furthermore, by adopting a new drum structure, it enables a greater reduction in protocol time than ever before. By using the HANABI P5, slides with minimal chromosome overlap can be created. This allows for a smoother analysis process after unfolding, achieving faster and more efficient routine work. 【Features】 ■ Creates an optimal environment for chromosome unfolding through our unique Dry-Index control ■ Can process up to 10 samples/20 slides in 30 minutes ■ Disposable chip specification ■ Equipped with a function for additional dropwise addition of fixative ■ Compact tabletop design that can be placed anywhere ■ ID code management for samples and slides (optional) ■ Tubes processed with the HANABI Harvester series can be set directly 　➢ Smooth transition from the cell harvesting process in chromosome analysis pre-treatment to the unfolding process.

• Other bio-related products

"HANABI P6" is a device that automates the unfolding process in chromosome analysis pre-treatment. Thanks to our unique Dry-Index control, it can create an environment suitable for chromosome unfolding. By using this product, anyone can produce chromosome metaphase specimen unfolding slides that are ideal for chromosome analysis such as FISH, CGH, and G-banding. The device is equipped with a drawer-type slide cassette, allowing for the replacement of slide glasses without opening the main cover. This has significantly reduced catch-up time and improved efficiency for each cycle. 【Features】 ■ Anyone can achieve good unfolding results ■ Provides stable unfolding results through Dry-Index control method ■ Sample liquid volume: 10-30μl/sample ■ Can produce five types of unfolding slides simultaneously ■ Easy replacement of slide glasses *For more details, please refer to the PDF document or feel free to contact us.

• Other bio-related products

The "HANABI-S1020" is a device designed to automatically perform the aging and staining processes, which are the preprocessing steps for chromosome analysis using the G-banding method. Typically, the aging process requires 2 to 3 days of standing, but this device implements it in about 30 seconds using a unique method. This significantly enhances the efficiency of routine tasks. Additionally, it can perform test runs before routine processing, allowing for the assessment of chromosome staining on a single processing slide before executing the full procedure. 【Features】 ■ Significantly enhances the efficiency of routine tasks ■ Processes up to 20 glass slides in about 30 minutes with a single operation ■ New glass slides can be set during operation ■ Test runs before processing are also possible ■ A compact tabletop device that does not require a lot of installation space ■ The slide cassette of HANABI P5 is designed to fit with the slide holder of HANABI S1020 　➢ Easily transitions to the staining process after the development process is completed

• Other bio-related products

The "MetaPhase Harvester HANABI P2P" is a device for preparing chromosome specimens that automatically executes the processing steps from cultured cells to cell harvesting. By performing the complex manual procedures uniformly, it achieves homogeneous harvesting processes. Additionally, it supports options such as reagent depletion detection sensors, waste tank sensors, the second Carnot line, and HANABI-SPI (automatic Carnot preparation device), allowing for more flexible protocol settings. 【Features】 ■ Can simultaneously process 24 samples in about 1.5 hours ■ Maintains constant temperature management in the centrifuge ■ Allows cell fixation processing with cooled fixative ■ Fully automates processes such as centrifugation, vortex mixing, and reagent injection ■ Supports options like reagent depletion detection sensors and waste tank sensors *For more details, please refer to the PDF materials or feel free to contact us.

• Other bio-related products

The "Metaphase Harvester HANABI P1" is an automatic cell harvesting device that automatically executes the processing steps from cultured cells to cell harvesting. It features a temperature-controlled centrifuge mechanism in the main operation unit and a pump that ensures stable liquid delivery, allowing for the dispensing of three liquids: PBS, hypotonic treatment solution, and Carnoy's fixative. By using this product, cell harvesting processes for the production of large quantities of stable chromosome metaphase specimens can be completed in a short time. 【Features】 ■ Simultaneous processing of 16 samples in about 1.5 hours ■ Temperature management of the centrifuge section ■ Preheat function for PBS solution/hypotonic solution ■ Cell fixation processing with cooled fixative ■ Built-in control panel *For more details, please refer to the PDF document or feel free to contact us.

• Other bio-related products

The "MetaPhase Harvester HANABI P3P" is a device that automatically executes the processing steps from cultured cells to cell harvesting. By applying the newly added simultaneous drainage and injection processing mode, it is possible to shorten the processing time by up to 15% (approximately 34 minutes). It can also display the remaining time for this process. This product supports a variety of options, significantly improving versatility and productivity. 【Features】 ■ Simultaneous processing of 64 samples in about 1.5 hours ■ Constant temperature management in the centrifuge section ■ Preheat function for PBS solution/hypotonic solution ■ Cell fixation processing with cooled fixative ■ Built-in control panel *For more details, please refer to the PDF materials or feel free to contact us.

• Other bio-related products

We would like to introduce our "HPLC Purification Columns & Buffers." By using two types of buffers for gradient elution, we achieve a reduction in analysis time. We offer sales starting from 2.5L bottles, and can produce larger quantities of 50L, 200L, and 900L to meet customer needs. Additionally, the columns are packed with alkylated polystyrene-divinylbenzene (PS-DVB) beads, allowing for the purification of both short and long nucleic acids. 【Features】 ■ Columns - Capable of purifying not only short nucleic acids but also long nucleic acids - Prevents impurities from remaining within the beads - Achieves high performance when used with dedicated buffers ■ Buffers - Can be mass-produced to meet customer needs - Reduces analysis time through gradient elution with two types of buffers The columns and buffers are also included in the USP mRNA analysis guidelines. *For more details, please refer to the PDF document or feel free to contact us.

• Other separation and analysis equipment
• High Performance Liquid Chromatograph
• Other purification and extraction