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We would like to introduce our "Biofilm Formation Suppression Evaluation Method on Substrates," which is part of our biofilm-related analysis. To evaluate the suppression of biofilm formation, the substrate is first immersed in a bacterial culture solution to allow biofilm formation. The formed biofilm is then stained with crystal violet (CV), after which the CV is extracted with an extraction solution, and the absorbance is measured. This is compared to the biofilm formation amount on a standard substrate. 【Analysis Method】 1. Immerse the substrate in a bacterial culture solution and incubate at a constant temperature for 24 to 48 hours. 2. Rinse the substrate with water and stain the formed biofilm with crystal violet. 3. Extract the crystal violet and measure the absorbance at 590 nm. 4. Compare with the biofilm formation amount on the standard substrate. *For more details, please refer to the PDF document or feel free to contact us.

We would like to introduce our analysis related to biofilms, specifically the "Evaluation Method for Bacterial Sterilization and Growth Inhibition in Biofilms." The sterilization effect is evaluated by measuring the metabolic activity of the bacteria. WST (water-soluble tetrazolium salt) is one of the reagents used to determine bacterial metabolic activity, producing WST formazan in accordance with the metabolic activity of the bacteria. By measuring the absorbance, we can determine the amount of surviving bacteria. 【Analysis Method】 1. Immerse the substrate or pin in the bacterial culture solution and incubate at a constant temperature for 24 to 48 hours to allow biofilm formation on the substrate or pin. 2. After immersing the substrate or pin in the sterilizing agent solution for a specified time, wash it with water. 3. Immerse the substrate or pin in the WST reagent for 10 to 60 minutes. 4. Measure the absorbance at 460 nm. 5. Compare the standard solution with the amount of biofilm. *For more details, please refer to the PDF document or feel free to contact us.

We would like to introduce our "Evaluation Method for the Destruction and Removal Effects of Biofilms," which is part of the biofilm-related analysis we conduct. When evaluating the function of cleaning agents that destroy and remove formed biofilms, we generally use a 96-well plate to allow biofilms to form on each well. The biofilms are then immersed in the cleaning agent for a specified period, and the amount of biofilm removal is compared against sterilized water (or sterilized buffer, etc.) as a control. 【Analysis Method】 1. Immerse the substrate or pins in a bacterial culture solution and incubate at a constant temperature for 24 to 48 hours to allow biofilm formation on the substrate or pins. 2. After immersing the substrate or pins in the biofilm cleaning agent for a specified time, rinse with water. 3. Stain the substrate or pins with crystal violet. 4. Extract the crystal violet and measure the absorbance at 590 nm. 5. Compare the biofilm amounts between the control and the samples. *For more details, please refer to the PDF document or feel free to contact us.

"Biofilm" is formed in environments where bacteria are present, provided there are adequate nutrients and moisture, and can develop not only at room temperature but also at low temperatures, characterized by its sliminess. Much of the sliminess is due to polysaccharides released by the bacteria. The majority of the volume consists of high molecular weight sugars (extracellular polysaccharides: EPS) that are produced by microorganisms. The type of biofilm that forms varies depending on the environmental conditions and the degree of bacterial species mixing and growth. [Examples of occurrence locations (partial)] ■ Bathroom and bathtub ■ Washing machine drum ■ Kitchen ■ Refrigerator ■ Air conditioner *For more details, please refer to the PDF document or feel free to contact us.

We would like to introduce the "Measurement Method for Biofilms" conducted by our company. Typically, to detect biofilms, a dye called crystal violet is used. This cationic blue dye compound strongly binds electrostatically to the anions of the biofilm constituents in aqueous solution. In organic solvents, the binding weakens, allowing the crystal violet that was bound to the biofilm to be extracted by the organic solvent. 【Other Methods】 ■ Sensory Testing ■ Gravimetric Method *For more details, please refer to the PDF document or feel free to contact us.

We would like to introduce what can be understood through biofilm analysis. It is utilized for evaluating the biofilm formation inhibition effects of panels and components used in various household appliances such as refrigerators, washing machines, and air conditioners, as well as for evaluating the biofilm formation inhibition effects of indwelling catheters. Our company has numerous achievements in evaluating biofilm formation suppression and removal capabilities using various materials and agents, so we can propose analysis systems tailored to your objectives and requests. 【Evaluation Achievements (Partial)】 ■ Evaluation of biofilm formation inhibition effects on materials used in bathtubs and piping ■ Evaluation of biofilm removal effects of cleaning agents ■ Evaluation of the inhibition effects on bacterial growth within biofilms by disinfectants, as well as evaluation of their sterilization effects ■ Evaluation of biofilm formation inhibition effects by surface treatment agents *For more details, please refer to the PDF document or feel free to contact us.

We would like to introduce the differences in biofilm formation by bacterial species based on the biofilm-related analyses conducted by our company. Many bacteria, from well-known ones like Escherichia coli and Pseudomonas aeruginosa to anaerobic bacteria such as periodontal pathogens, form biofilms. While the formation is influenced by culture conditions, the amount and structure of biofilm formation vary by species. Additionally, the biofilm formation by mixed bacteria can significantly exceed that of single species, and their morphology can differ greatly. [Differences by Bacterial Species] ■ Bacterial species with high biofilm formation: Pseudomonas aeruginosa, Staphylococcus epidermidis, Streptococcus mutans ■ Bacterial species with low biofilm formation: Escherichia coli, Staphylococcus aureus ■ Bacterial species that form highly adhesive biofilms: Staphylococcus epidermidis ■ Bacterial species that form less adhesive biofilms: Pseudomonas aeruginosa *For more details, please refer to the PDF document or feel free to contact us.

We would like to introduce the removal of biofilms using sodium hypochlorite solution based on the biofilm-related analysis conducted by our company. The effectiveness is quicker at higher concentrations, and at a 5% concentration, biofilms can be removed in just a few minutes. The effectiveness of biofilm removal also varies depending on the type of bacteria and the amount of biofilm present. It is also important to properly control the amount of biofilm formation to evaluate the effectiveness of the cleaning agent. 【Features】 ■ The higher the concentration, the quicker the removal; at a 5% concentration, removal is possible in just a few minutes. ■ The amount of biofilm decreases in a concentration-dependent manner with sodium hypochlorite. *For more details, please refer to the PDF document or feel free to contact us.

We would like to introduce our "Biofilm Evaluation Using Mixed Bacteria." Typically, to measure the anti-biofilm activity of agents or treatment substrates, biofilm formation is conducted using specific strains such as Staphylococcus epidermidis or Pseudomonas aeruginosa, cultured on media used for each bacterium. However, if you want to evaluate the effectiveness in a specific environment, it is also possible to use biofilms formed from bacteria collected from that environment. At our company, we conduct biofilm formation experiments using microbial communities collected from sinks and bathtubs, allowing for evaluations under conditions that are more relevant to the environments in which they will be used. Please feel free to contact us for more details. [Features] ■ If you want to evaluate effectiveness in a specific environment, it is possible to use biofilms formed from bacteria collected from that environment. *For more details, please refer to the PDF document or feel free to contact us.

The "slime" that occurs in sinks in kitchens and bathrooms is called biofilm. If cleaning is not thorough, it can become slippery before you know it, and if left unattended, it can proliferate to the point where water cannot flow. This creates a negative image of being dirty and troublesome. This time, we will introduce the analysis methods. The analysis of biofilm is expected to be utilized for various purposes, such as developing materials used in water areas like bathtubs and kitchens, developing agents that inhibit biofilm formation, and examining conditions to efficiently produce biofilm as a useful component. *For detailed information, please refer to the related links and catalog. Feel free to contact us for more information.

Biofilms exist in various places around us. For example, the surface of teeth, the kitchen and bathroom sinks, and the slime in the bathtub are all examples of biofilms. In daily life, the presence of biofilms poses issues such as the unpleasantness of slime, the hassle of cleaning, and the risk of infection, leading to the sale of many products designed to eliminate the bacteria that cause biofilms and suppress their occurrence and formation. However, within just a few days, the proliferation of surviving bacteria leads to the reformation of biofilms, creating a cycle of ongoing struggle. This time, I have summarized information about biofilms. *For detailed content of the article, please refer to the related links and catalog. For more information, feel free to contact us.*