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同仁グローカル

EstablishmentApril 1990
capital2000Ten thousand
addressKumamoto/Mashiko-cho, Kamimashiki-gun/2081-25 Tahara, Kumamoto Techno Research Park
phone096-286-1311
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last updated:Aug 05, 2025
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同仁グローカル List of Products and Services

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Biofilm Biofilm
Food foreign matter Food foreign matter
Enzyme activity analysis Enzyme activity analysis
Antioxidant capacity measurement Antioxidant capacity measurement
Various commissioned analyses Various commissioned analyses
Fatty acid analysis Fatty acid analysis
Trivia Trivia
Fatty

Fatty acid analysis

- I don't know the sample pretreatment methods or how to analyze fatty acids. - I want to perform fatty acid analysis in research, product development, and quality control. - I want to analyze trace amounts of fatty acids in the sample. - I want to measure changes in fatty acid composition. - I need fatty acid analysis, but the pretreatment and analysis are cumbersome. - I want to analyze organic acids (such as formic acid, acetic acid, butyric acid, etc.).

What can be understood through fatty acid analysis

The types of lipid molecules that make up lipids are vast! Analyzing lipids is typically done by analyzing them as fatty acids.

Our company offers "fatty acid analysis" for trace samples such as blood cells and other precious samples. Measuring the proportion of each fatty acid contained in lipids and the amount of each fatty acid can provide insights into the condition of each sample. Fatty acid analysis may yield new findings, so please feel free to contact us. [Analysis Objectives (Excerpt)] ■ Meat: Evaluation of fatty acid composition ratios based on tissue/species differences ■ Plants: Evaluation of fatty acid composition ratios based on species differences ■ Microorganisms: Evaluation of fatty acid composition based on differences in habitat ■ Algae: Measurement of fatty acid content and composition ratios ■ Tea: Evaluation of fatty acid composition ratios based on processing methods *For more details, please refer to the PDF document or feel free to contact us.

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Lipid fractionation: Fatty acid analysis by type of lipid (TLC)

Useful for preliminary evaluation when planning experiments! Introduction to fractionation using thin-layer chromatography (TLC).

We would like to introduce our "Fatty Acid Analysis by Type of Lipid" that we conduct. The extracted lipids contain various lipid classes with different properties, and by fractionating them by class, it becomes possible to focus the analysis on the desired fraction. TLC is a method that uses a stationary phase, such as silica gel coated on glass or aluminum, and a mobile phase using organic solvents to separate and fractionate substances. 【Point: What can be understood from TLC fractionation】 ■ I want to get an overview of what types of lipids are included. ■ I want to understand the lipid composition and approximate quantities. ■ I want to separate the desired lipid class. ■ I want to confirm the quantitative changes in lipid classes. *For more details, please download the PDF or feel free to contact us.

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Analysis of fatty acid composition

Analysis using standard samples of three components including oleic acid, palmitic acid, vaccenic acid, EPA, and DHA!

We would like to introduce our "Fatty Acid Composition Analysis." The detection sensitivity for each fatty acid varies depending on the equipment used. To correct for these differences, we measure standard samples to determine correction factors and calculate the corrected area from the measured area. We then use this corrected area to determine the content of each fatty acid. We conduct fatty acid analysis on a variety of samples, including fresh foods, grains, processed and fermented foods, animal tissues, plant tissues, blood, cells, bacteria, culture media, feed, and excrement. 【Test Overview】 ■ Target Fatty Acids - Analysis is conducted using standard samples for 36 components, including oleic acid, palmitic acid, vaccenic acid, EPA, and DHA. ■ Data - Reports are provided along with an Excel file. *For more details, please download the PDF or feel free to contact us.

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Quantitative analysis of fatty acids (by GC/MS)

Regarding the data, we also provide a report in Excel format along with the report!

Equipment Used: GC/MS Our company conducts "quantitative analysis of fatty acids" contained in lipids. We create a calibration curve using standard samples to calculate the fatty acid concentration. The fatty acid concentration will be reported in units such as "mg/g" or "mg/mL." For the target fatty acids, we perform quantitative analysis using all standard samples for 36 components, including oleic acid, palmitic acid, vaccenic acid, EPA, and DHA. We also conduct quantitative analysis for specific fatty acids. [Point] ■ Changes in fatty acid levels during food processing ■ Differences between wild types and genetically modified organisms ■ Changes in cell quantities due to culture conditions ■ Changes in fatty acid levels in the medium before and after culture *For more details, please download the PDF or feel free to contact us.

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[Analysis Case] Fatty Acid Analysis of Animal Tissue (Cells)

We will propose analyses tailored to your objectives, including sample preprocessing methods!

Using Equipment: GC/MS We would like to introduce our "Fatty Acid Analysis of Animal Tissues (Cells)." We conduct analysis of fatty acid composition in tissues such as muscle, fat, and organs, as well as in blood cells and cultured cells, and analyze specific fatty acid components. Our company performs fatty acid analysis on various samples including fresh foods, grains, processed and fermented foods, animal tissues, plant tissues, blood, cells, bacteria, culture media, feed, and waste. [Analysis Example] ■ Cultured Cells: Human Immortalized Keratinocytes *For more details, please download the PDF or feel free to contact us.

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[Analysis Case] Fatty Acid Analysis of Plant Tissues

Using the machine: GC/MS We are conducting analyses of the fatty acid composition of common edible oils!

We would like to introduce our "Fatty Acid Analysis of Plant Tissues." We conduct analysis of the fatty acids contained in plant tissues (cells). Measuring the proportion of each fatty acid in lipids and the quantity of each fatty acid can provide insights into the condition of each sample. Please feel free to contact us when you need our services. [Analysis Examples] ■ Edible Oil Fatty Acids ■ Olive Oil *For more details, please download the PDF or feel free to contact us.

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[Analysis Case] Fatty Acid Analysis of Microorganisms

Using the machine: GC/MS. It is also possible to analyze branched fatty acids and hydroxy fatty acids contained in microorganisms!

We would like to introduce our "Fatty Acid Analysis of Microorganisms." We conduct fatty acid analysis of lipids from microorganisms such as bacteria, plankton, and algae. Measuring the proportion of each fatty acid contained in the lipids and the amount of each fatty acid helps us understand the condition of each sample. We perform fatty acid analysis on various samples, including fresh foods, grains, processed and fermented foods, animal tissues, plant tissues, blood, cells, bacteria, culture media, feed, and excrement. [Analysis Example] ■Microorganism: Staphylococcus epidermidis *For more details, please download the PDF or feel free to contact us.

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[Analysis Case] Analysis of Fatty Acids on the Surface of Insects

Using the machine: GC/MS, we are analyzing the fatty acid components on the surface of insects and the fatty acid components contained in microorganisms!

Our company conducts "fatty acid analysis of the insect body surface." Insects are covered by an exoskeleton, and lipid components are secreted on their surface as wax to protect them from drying out. Additionally, it is believed that there are fatty acid compositions related to the unique individual recognition of insects. In the downloadable materials, you can find the results of the extraction and fatty acid analysis of the lipids from the body surface of stink bugs. Please take a look. [Analysis Examples] - Conducted analysis of fatty acid components on the insect body surface and fatty acid components in microorganisms. - Analysis Method: - Extraction: Chloroform-methanol mixed extraction - Derivatization: Methyl esterification - Analysis Equipment: GC/MS (Agilent Technologies) *For more details, please download the PDF or feel free to contact us.

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[Analysis Case] Differences in Fatty Acid Composition by Fish Parts

Use of machinery: GC/MS Introduction to fatty acid composition analysis of the abdominal, dorsal, skin, and periorbital (gelatinous part) regions of the sardine.

We would like to introduce the measurement of "differences in fatty acid composition by fish part" that we conduct. Measuring the proportion of each fatty acid contained in the lipids of each part and the amount of each fatty acid allows us to understand the differences in the proportions of fatty acids in each part. Here, we present the results of measurements regarding the differences in fatty acid composition of various parts of the fish. [Analysis Example] ■ Fatty acid composition analysis of each part of the Pacific saury (raw) ■ Parts: Flesh (belly), Flesh (back), Skin, Around the eyes *For more details, please download the PDF or feel free to contact us.

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[Analysis Case] Differences in Fatty Acid Composition by Type of Tea Leaves

Using the machine: GC/MS, we will propose analyses tailored to your needs, including sample pretreatment methods!

We would like to introduce the measurement of "differences in fatty acid composition by type of tea leaves" that we conduct. Tea is basically made from the same tea leaves, but the manufacturing processes differ. Sencha is made without fermentation, while oolong tea and black tea undergo fermentation processes. As a result, the proportions of fatty acids in the lipids contained in each type of tea and the amounts of each fatty acid vary. Here, we present the results of measurements regarding the differences in fatty acid composition by type of tea leaves. [Analysis Example] - Analysis of fatty acid composition by type for sencha, matcha, oolong tea, and black tea. *For more details, please download the PDF or feel free to contact us.

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