サーモフィッシャーサイエンティフィック株式会社／Thermo Fisher Scientific K.K. Product Lineup

As a separation method in ion chromatography, ion exchange is mainly used. Understanding the principle of ion exchange will help in adjusting the separation to meet measurement objectives and in selecting the appropriate column. This introduces the ion exchangers packed in columns for ion chromatography.

• Analytical Equipment and Devices

Ion exchange separation is the fundamental mechanism of separation in ion chromatography, where ionic components repeatedly adsorb and desorb between the ion exchange sites and the electrolyte solution. This principle and its characteristics will be explained.

• Analytical Equipment and Devices

Ion exchange separation occurs due to the competition between the measurement ions and the eluent ions on the ion exchange sites. This explains the concentration and types of eluent that affect this ion exchange separation.

• Analytical Equipment and Devices

Ion exchange separation occurs due to the competition between the measurement ions and the eluent ions on the ion exchange sites. One of the factors that affects this ion exchange separation is the change in column temperature. When the column temperature is altered, the retention time of the measurement ions changes due to variations in separation equilibrium, diffusion rates, degree of dissociation, and the viscosity of the eluent. The impact of temperature varies depending on the type of measurement ions and also changes with different columns and eluents. This factor of temperature will be explained.

• Analytical Equipment and Devices

Ion exchange separation occurs due to the competition between the measured ions and the eluent ions on the ion exchange sites. One factor that affects this ion exchange separation is hydrophobic interaction. I will explain this factor of hydrophobicity.

• Analytical Equipment and Devices

Ion exchange separation occurs due to the competition between the measurement ions and the eluent ions on the ion exchange sites. One of the factors that affects this ion exchange separation is the change in the flow rate of the eluent. I will explain the differences in the chromatograms obtained by varying the flow rate of the eluent and the points to be cautious about.

• Analytical Equipment and Devices

If optimization of separation cannot be achieved by adjusting the elution liquid or temperature, it is recommended to change the type of column. Using columns with different performance can significantly alter the separation depending on the ionic components. We will explain our cation columns.

• Analytical Equipment and Devices

If optimization of separation cannot be achieved by adjusting the elution solution or temperature, it is recommended to change the type of column. Using columns with different performance can significantly alter the separation depending on the ionic components. We will explain our anion columns.

• Analytical Equipment and Devices

Optimizing separation requires effort and time. Therefore, we introduce the Thermo Scientific Virtual Column Separation Simulator, which can simulate the optimization of separation on a computer.

• Analytical Equipment and Devices

The Thermo Scientific Chromeleon Chromatography Data System (CDS) simplifies the workflows of customers engaged in analytical operations, helps deepen the understanding of data, and achieves ease of use, comprehensive instrument control, automated data analysis, and flexible reporting. In preparation for next-generation lab environments, it also supports the management of complex mass spectrometry data and regulatory compliance. It is compatible with third-party LC and GC systems, allowing for centralized management of the entire chromatography environment in multi-vendor labs. By centralizing the management of tasks related to analysis, from execution to analysis, report output, and data management, it strongly supports compliance with regulations such as 21 CFR Part 11 and GxP.

• Analytical Equipment and Devices
• Software (middle, driver, security, etc.)
• Integrated operation management

The Reagent-Free IC (RFIC) eluent generator system is a groundbreaking system that allows for the inline preparation of eluent at the required concentration simply by sending ultra-pure water from a pump. It not only eliminates the hassle of eluent preparation but also enables the supply of accurate and high-purity eluent. Additionally, gradient analysis can be easily performed with just the concentration settings from the workstation.

• Analytical Equipment and Devices

The "GENESYS Series" is a UV-Vis spectrophotometer developed based on our over 60 years of experience in the field of spectroscopy. Designed specifically for use in laboratories and classrooms where robust and easy-to-operate equipment is required, the Thermo Scientific GENESYS series spectrophotometers cater not only to routine analysis but also to a wide range of applications. With high operability and flexibility, and a variety of accessories tailored to meet different analytical needs, experiments can be conducted more easily. 【Features】 ■ Equipped with a high-resolution color display, achieving simple and easy operability ■ Adopts a large sample compartment for easy access during sample exchange ■ Allows selection of multiple data output methods, enabling flexible and smart data output ■ All models in the GENESYS series come with Japanese software ■ The UV-Vis models of the GENESYS series* do not require a warm-up period after power-on (*Applicable models: GENESYS 50 / GENESYS 150 / GENESYS 180 / Biomate 160)

• Spectroscopic Analysis Equipment

■Sales Performance Boasting Top Market Share■ Since starting sales of ion chromatography in Japan in 1975, we have pursued ease of use and have delivered over 4,000 units nationwide. We boast a market share of over 65%. *Source: R&D Corporation, Scientific Instruments Yearbook ■High-Level Customer Support System■ To ensure that our equipment can be used stably and for a long time, we collaborate with sales agents nationwide and have over 90 certified service engineers. Additionally, technical support is available from resident engineers, allowing you to use our products with peace of mind. ■Reliable Technical Expertise Supporting Analysts and Labs■ Advanced technologies such as a mobile phase generator capable of automatic generation of elution solutions and suppressors contribute to the efficiency of lab operations. We offer over 90 types of columns for ion chromatography.

• Analytical Equipment and Devices

The "Quasor II EBSD System" is an electron backscatter diffraction analysis device that allows for the simultaneous collection of EBSD data and EDS/WDS spectral images with ease. It characterizes the crystal structures that influence the physical properties of a wide range of materials observed through scanning electron microscopy (SEM) and microanalysis. You can obtain both structural and chemical information of the sample in a single measurement. 【Features】 ■ Collection speed of up to 600 frames per second with 99% of pixels indexed ■ Obtain both structural and chemical information of the sample in a single measurement ■ Background removal through subtraction or division ■ Image cropping ■ Flat fielding using FFT or parabolic methods *For more details about the product, please contact us directly.

• Analytical Equipment and Devices

The "WDS MagnaRay Spectrometer" enables elemental analysis in electron microscopes, automatic intelligent alignment, and parameter settings with high speed and reliability. High-resolution trace analysis can be performed as easily as with an EDS system. The built-in expert system automatically handles alignment, analysis settings, and data collection. 【Features】 ■ High resolution ■ Sensitivity to trace elements ■ Continuous spectrometer covering the entire spectral range ■ Guaranteed sensitivity specifications *For more details about the product, please contact us directly.

• Spectroscopic Analysis Equipment
• Analytical Equipment and Devices

"Pathfinder" is a new tool designed to achieve efficient and comprehensive electron microscope analysis. It allows for the detection of trace elements in extremely complex matrices by scanning spectral imaging data at the pixel level. Our proprietary 29 types of imaging filters enhance the clarity and comprehensibility of SEM/EDS and SEM/WDS data images. 【Features】 ■ Achieves efficient and comprehensive electron microscope analysis ■ Prevents prolonged and subjective analysis ■ Reliable analysis can be completed in less than one minute ■ Excellent for discovering the properties of unknown materials and foreign substances ■ Capable of detecting trace elements in extremely complex matrices *For more details about the product, please contact us directly.

• Software (middle, driver, security, etc.)
• Analytical Equipment and Devices

The "UltraDry EDS Detector" provides faster and more accurate X-ray interpretation in industries such as metals and mining, advanced materials, and semiconductors. When used in conjunction with Thermo Scientific Pathfinder X-ray microanalysis software, it demonstrates exceptional resolution with a very high yield. It maintains an extremely high data collection speed without the need for external cooling or cooling with liquid nitrogen. 【Features】 ■ Incredible operating space that achieves excellent performance ■ Miniaturized integrated FET to effectively eliminate electronic noise ■ One of the largest collection solid angles among detectors available on the market, thanks to the crystalline active area ■ Sensitivity to light elements down to Be (beryllium) ■ No auxiliary cooling such as cooling water or fans, or liquid nitrogen required *For more product details, please contact us directly.

• X-ray inspection equipment
• Other inspection equipment and devices

The "ESCALAB Xi+" is an imaging XPS microprobe that achieves ultimate high sensitivity and energy resolution through the adoption of advanced technology. A wide variety of options are available to meet diverse needs. From system control to data measurement, data analysis, and report generation, various functions are seamlessly integrated by the Thermo Scientific Avantage data system, enabling easy operation and high productivity. 【Features】 ■Ultimate performance ■Efficient operation ■Flexible design *For more details, please refer to the PDF document or feel free to contact us.

• probe
• Analytical Equipment and Devices

The "NanoDrop Series" is an ultra-micro UV-Vis spectrophotometer that enables concentration measurement with just 1μL. Its unique sample retention system allows for direct measurement of high-concentration samples without the need for dilution or cuvettes. The highly polished sample pedestal is made of hydrophobic stainless steel, allowing for easy wiping between measurements, eliminating concerns about sample carryover. 【Features】 ■ Concentration measurement with just 1μL ■ Eliminates waste in sample measurement and provides accurate results in seconds ■ Direct measurement of high-concentration samples without dilution ■ No worries about sample carryover ■ Only 3 simple steps: "Apply sample, measure, wipe" *For more details, please refer to the PDF document or feel free to contact us.

• Spectroscopic Analysis Equipment

The "NanoDrop One" is an ultra-micro spectrophotometer that incorporates the new Thermo Scientific Acclaro sample intelligence technology, further expanding the limits of spectroscopic analysis. This technology allows for accurate assessment of sample quality, enabling the progression of downstream application experiments. It supports faster analysis, taking one step further towards our customers' success. 【Features】 ■Convenience ready to use ■Minimal sample preparation ■No consumables required ■Rapid and easy measurements ■Rich data management options *For more details, please refer to the PDF materials or feel free to contact us.

• Spectroscopic Analysis Equipment

"Nexsa" is an X-ray photoelectron spectroscopy device that achieves both the highest level of performance and complete automation of measurements. The new monochromator X-ray source can measure from 10 to 400 μm in 5 μm steps while maintaining high sensitivity. The combination of a high-efficiency lens system and detector enables high-sensitivity, high-speed XPS analysis. 【Features】 ■ Capable of measuring low-concentration compounds and microscopic areas ■ Achieves both the highest level of performance and complete automation of measurements ■ Multi-functional options can be equipped ■ Realizes high-sensitivity, high-speed XPS analysis ■ Makes it easy to measure insulators *For more details, please refer to the PDF document or feel free to contact us.

• Spectroscopic Analysis Equipment

The K-Alpha is a fully automated X-ray photoelectron spectroscopy device that achieves the highest level of operability and unparalleled performance simultaneously through the adoption of new technology. Equipped with a low-power, high-intensity X-ray monochromator, it allows for the selection of analysis areas in 5μm steps from 50μm to 400μm. By adjusting the analysis area to the desired sample size, it is possible to obtain the highest level of signal intensity. 【Features】 ■ High sensitivity, high energy resolution XPS measurements ■ Micro-focus X-ray source for micro-area analysis ■ Real-time sample observation mechanism from the vertical direction of the sample ■ Excellent operability and high throughput ■ Integrated control of measurement, analysis, and device control through the Thermo Scientific Avantage data system *For more details, please refer to the PDF document or feel free to contact us.

• Spectroscopic Analysis Equipment

The ARL iSpark Plus OES is a high-performance emission spectrometer based on a sophisticated photomultiplier tube (PMT) optical system. This device features excellent functionality and other innovative technologies, as outlined below: • Rapid analysis (15% faster than conventional methods) • Unique PMT optical system • Groundbreaking digitally controlled emission device • Innovative spark stand design – Improved maintainability – Enhanced stability for almost all elements – Minimal memory effect • Advanced acquisition technology and algorithm processing for PMT signals • Single spark data collection capability with an algorithm to remove the intensity data of scattered sparks, improving PMT accuracy • Cutting-edge OES analysis of non-metallic inclusions • High-performance argon gas management with argon gas-saving mode

• Spectroscopic Analysis Equipment

The HAAKE MARS 40/60 rheometer is designed for high versatility, aimed at measuring samples in various states from liquids to solids. Additionally, by combining it with other analytical instruments (such as FT-IR and Raman spectrometers), it is possible to measure not only physical properties but also the chemical state of the samples, making it useful for research and development in various fields. 【Features】 ■ Convenient pneumatic rotor release ■ TCP/IP Ethernet data communication for rapid data acquisition ■ Remote control and maintenance via a password-protected web server ■ A wide range of accessories to accommodate physical property evaluation of all samples ■ Connect assist function for quick accessory exchange ■ Measurement geometry optimized for accurate sample loading *For more details, please refer to the PDF document or feel free to contact us.

• Other measurement, recording and measuring instruments

The rheometer "HAAKE Viscotester iQ" can operate in four modes despite its compact design, allowing for both rotational and dynamic measurements with settings available for either stress control or strain control. It is suitable for sampling inspections in QC, routine measurements, and even extensive rheological measurements. From beginners to experts, anyone can easily and reliably obtain accurate measurement results. 【Features】 ■ Intuitive operability that requires no explanation from setup to measurement tasks ■ Easy, accurate, and highly reproducible gap settings with a smart lift function ■ A powerful, high-resolution, high-response EC motor enables flexible measurements ■ Various measurement geometries accommodate a wide range of measurement samples ■ Equipped with an easy-to-read color panel for numerical data and graphs *For more details, please refer to the PDF document or feel free to contact us.

• Other measurement, recording and measuring instruments

The "Thermo Scientific HAAKE MARS iQ Rheometer Series" offers advanced flexibility and ease of use to meet a wide range of needs in quality control and research and development. Regardless of the user's proficiency, it is possible to consistently evaluate the characteristics of a wide variety of samples in a short time. The user-friendly software can be easily operated on a touchscreen, and thanks to its clear graphical display, users can reliably adhere to standard operating procedures (SOPs). 【Features】 ■ The Connect Assist function ensures the correct measurement geometry is selected, enabling accurate measurements without errors. ■ Configurations can be flexibly and quickly changed to suit various analytical purposes. ■ Two models are available, featuring ball bearings and air bearings. ■ Capable of texture analysis, tribology measurements, pressure dependency measurements, and more. ■ The adoption of a high-rigidity frame significantly improves reliability. ■ Environmentally friendly materials, low power consumption, and resource-efficient manufacturing processes are utilized. *For more details, please refer to the PDF document or feel free to contact us.

• Other measurement, recording and measuring instruments

The Thermo Scientific Antaris II near-infrared analyzer achieves innovative analytical standards for raw material acceptance testing, final product verification, and simultaneous multi-component quantitative analysis. In addition to its intuitive operability that anyone can use, it boasts exceptional stability and data reproducibility. With a compact and robust design that can be used in harsh environments such as manufacturing lines and on-site locations, it also excels in maintainability. 【Features】 ■ Rapid transfer of analytical methods from the laboratory to the field system ■ Reduction of maintenance costs ■ Easy-to-use measurement menus and automated validation software ■ Compliance with official analytical methods and validation programs in Europe and the United States ■ Comprehensive global support program *For more details, please download the catalog or feel free to contact us.

• Analytical Equipment and Devices

The Thermo Scientific Nicolet Summit series FTIR can quickly and reliably identify, verify, and quantify samples, improving the productivity of routine analytical tasks. Combining the newly developed optical system, LightDrive Optical Engine, with the innovative Paradigm software, the Nicolet Summit FTIR can identify, verify, and quantify samples more quickly and reliably than ever before, enhancing the productivity of everyday analytical work. It offers three product lines: Summit PRO, Summit X, and Summit LITE. Please choose the optimal model for your purpose. 【Features】 ■ Space-saving and robust design ■ Suitable as a shared device for multiple users ■ Touchscreen monitor that can be directly attached to the instrument ■ Multi-color LED indicators allow for immediate understanding of the device status, enabling quick assessment of analytical results.

• Analytical Equipment and Devices

The "Thermo Scientific Nicolet iS50 Series FT-IR" is our flagship FTIR instrument, featuring high expandability and flexibility. With simple and intuitive operation, it quickly and easily provides reliable data. We offer the "Nicolet iS50" and the "Nicolet iS50R," which comes standard with a step scan function. 【Features】 <Nicolet iS50> ■Thermo Scientific Polaris mid-infrared light source ■Gold-coated optics ■High expandability to accommodate various applications ■Diverse electric control options ■High-quality design *For more details, please refer to the PDF document or feel free to contact us.

• Spectroscopic Analysis Equipment

The "Thermo Scientific Nicolet Apex FTIR" is widely compatible with everything from routine analysis to diverse research applications. Designed with advanced technology, the LightDrive optical engine delivers next-generation analytical performance. The interferometer, laser, and infrared light source come with a 10-year warranty. Additionally, its excellent S/N ratio and wavenumber resolution reduce the time required for data collection. • High levels of performance and excellent S/N ratio and spectral resolution meet all needs. • The simple and intuitive Thermo Scientific OMNIC Paradigm software enables infrared spectral measurements tailored to various requirements. • A streamlined validation workflow provides audit managers with methods compliant with pharmacopoeias (Ph.Eur., USP, JP, CP). • With the cloud-based OMNIC Anywhere software, users can analyze data anytime, anywhere, and from any device. *For more details, please refer to the PDF document or feel free to contact us.

• Spectroscopic Analysis Equipment

The "Thermo Scientific Nicolet iN10 Micro FT-IR" is a next-generation high-sensitivity dedicated micro system. It features easy operation with a focus on usability. The all-in-one system eliminates the need for a separate FT-IR unit. Additionally, we offer the fully automated microscope "Thermo Scientific Nicolet iN10 MX Infrared Imaging System" with software control. 【Features】 ■ Real-time observation of visible images during measurement ■ Achieves speedy analysis ■ Automatic component analysis and particle analysis functions convenient for separating mixtures ■ Multi-coated Ge Tip-ATR provides high sensitivity with over 50% throughput ■ Easy operation for measurement, analysis, and searching with the analysis wizard *For more details, please refer to the PDF document or feel free to contact us.

• Optical microscope

The "Thermo Scientific Nicolet iN5 Micro FT-IR" has achieved intuitive and easy operation. It features a highly reliable optical system that requires no alignment, high-output LED illumination, and the ability to clearly identify areas of interest on the sample using an eyepiece or camera (optional). You can select the most suitable analytical method (transmission, reflection, ATR) according to the sample and quickly obtain high-quality data. 【Features】 ■ Multiple visible observation options available ■ High-output LEDs provide clear sample illumination for extended periods ■ An aperture positioned at the front of the device ensures precise pinpoint identification of the analysis target ■ A fixed objective lens maintains a consistently aligned optimal state ■ A focus adjustment knob at the front of the device makes it easy to adjust the focus on the sample *For more details, please refer to the PDF document or feel free to contact us.

• Optical microscope

The "Thermo Scientific DXR3 Series Raman Spectrometer" is a system that anyone can master without the need for experience or knowledge. With its patented optical axis adjustment feature, it always maintains optimal conditions. Additionally, the intuitive software, which is thoroughly designed for ease of use, reduces the time required for analysis and flexibly accommodates various analytical purposes. 【Features】 ■ Changing lasers, filters, and gratings is very easy ■ All optical components are managed by Thermo Scientific OMNIC, ensuring compatibility at all times ■ Can be connected to a PC via USB port, allowing operation with a laptop ■ Automatic mirror adjustment maintains optimal peak intensity at all times ■ Adjusts laser output to achieve consistently stable Raman intensity *For more details, please refer to the PDF document or feel free to contact us.

• Spectroscopic Analysis Equipment

This application note presents a case study of the high-precision separation and collection of cis/trans isomers of vitamin K1 using the Thermo Scientific Vanquish Core HPLC system and Vanquish Fraction Collector. The normal phase kit extends compatibility with organic solvents such as hexane, confirming excellent reproducibility that meets EP standards (RSD < 1%). Recovery rates of 99.5% to 101.4% were achieved for both peak-based and time-based collections. Furthermore, collection and storage were conducted under temperature control, comparing isomerization behavior at 8 °C and 35 °C, demonstrating that temperature management is crucial for suppressing structural changes. For more details on reliable isomer purification using the normal phase compatible collection system, please refer to the materials. 【Features】 ■ Extended solvent compatibility with normal phase HPLC compatible kit ■ High reproducibility with retention time and peak area RSD < 1% ■ High-precision collection with recovery rates of 99.5% to 101.4% ■ Significant suppression of isomerization with low-temperature storage at 8 °C ■ Quantitative evaluation of isomerization reactions and verification of temperature dependence

• Analytical Equipment and Devices

The Thermo Scientific Vanquish Fraction Collector (VFC) is a system that enables peak-triggered fractionation using UV, CAD, and MS detectors. With CAD and MS detection, it has become possible to fractionate and evaluate unknown components and impurities that were difficult to detect with conventional UV detection. This application note explains the features of the VFC equipped with innovative valve technology, using examples from plant extracts. It provides detailed practical know-how from parameter settings for accurate fractionation to applications on a semi-preparative scale. 【Features】 ■ Low contamination: Cleaning functions for needle and buffer loop ■ High precision fractionation: Automatic calculation of delay volume, peak-triggered fractionation ■ Excellent versatility: Diverse fractionation settings using UV, CAD, and MS detection ■ High reliability: Fractionation enabled by innovative valve technology

• Analytical Equipment and Devices

The HPLC Charged Aerosol Detector (CAD) system is a solution that allows for the rapid and highly accurate quantification of lipids in liposome formulations. This technical sample presents the results of validation using the ASTM method across 12 system configurations at 4 sites (Thermo Scientific Vanquish Flex UHPLC and Vanquish Core HPLC). Reliable results were obtained across all systems, and they passed the system suitability tests. Additionally, excellent separation performance was confirmed using the Thermo Scientific Hypersil GOLD column. 【Features】 ■ High precision quantification: Quantification is possible in the range of 0.2 to 300 µg/g using linear, quadratic, and log-log calibration curves. ■ High reliability: Passed system suitability tests at all sites. ■ Excellent performance: High separation capability and rapid analysis with the Hypersil GOLD column. ■ Efficient analysis: Consistent responsiveness and high-precision results obtained quickly.

• Analytical Equipment and Devices

This application note introduces the analysis of polymer compounds using size exclusion chromatography (SEC) with the Vanquish Charged-Aerosol Detector (CAD). The CAD has approximately 100 times the sensitivity compared to a differential refractive index detector (RI) and is a versatile detector capable of accurately detecting semi-volatile and non-volatile components. Its wide dynamic range and excellent stability make it ideal for analyzing molecular weight distributions. This document discusses the detection of calibration standard samples and calibration curves, comparisons of sensitivity, and changes in retention time due to sample concentration, using polystyrene samples to compare RI and explain the characteristics and advantages of CAD. 【Features】 ■ High sensitivity detection: CAD achieves approximately 100 times the sensitivity compared to RI ■ Wide dynamic range: Accurately measures samples of diverse molecular weights ■ Detection in the polymer range: Effectively detects polymers with molecular weights up to 400,000 ■ Low concentration measurement: Minimal concerns about carryover or column loading even at low concentrations ■ Stability: Provides rapid stabilization and consistent results

• Analytical Equipment and Devices

In the quality control of plating solutions, the quantification of additives is an important factor. In particular, the quantification of sulfur-containing compounds that contribute to film quality adjustment and prevention of liquid decomposition is essential. This document provides accurate and highly reproducible analytical results using a method that does not require complex pretreatment. For the HPLC measurement conditions, an ion-pair reagent was added to the mobile phase, and an injection method was adopted to reduce the influence of sample impurities. As a result, sharp peaks can be obtained without being affected by metals and inorganic ions. Additionally, by comparing the chromatograms of the standard solution and the plating solution sample, it can be confirmed that the concentration of additives is maintained consistently. Furthermore, as an evaluation of reproducibility, the same sample was measured five times, demonstrating high reproducibility. 【Features】 ■ High-sensitivity quantitative analysis without complex pretreatment ■ High reproducibility using ion-pair reagents ■ Injection method that reduces the influence of metals and inorganic ions ■ Accurate quantification through comparison of standard solutions and actual samples ■ Highly reproducible analytical results

• Analytical Equipment and Devices

The Thermo Scientific Vanquish Split Sampler significantly reduces the effort and time associated with traditional manual dilution by allowing users to set up an automated dilution program for samples. This technical note verifies the accuracy and reproducibility of automated dilution of standard solutions through the measurement of DNPH-derivatized formaldehyde. Custom injections using user-defined programs enable easy execution of multi-step dilutions and automation of calibration curve creation. Furthermore, by utilizing the e-workflow of Thermo Scientific Chromeleon CDS, the entire process from analysis to report generation can be greatly streamlined, enhancing the reliability of analyses and increasing laboratory productivity. **Features** - High reproducibility and accuracy through automated dilution - Flexible dilution settings with custom injection programs - Automation of analysis with Chromeleon CDS e-workflow, eliminating human error - Significant reduction in effort and time compared to manual dilution - High-precision preparation capable of up to 20-fold dilution

• Analytical Equipment and Devices

This document introduces a method for the high-precision separation and quantification of four types of tocopherols contained in vegetable oils using the single-loop heart-cut 2D-LC method. Under the separation conditions of a reverse-phase system using a general ODS column, the separation of β-tocopherol and γ-tocopherol is difficult; however, the 2D-LC method allows for easy separation. In the first dimension, tocopherols and interfering components in the sample are separated, and in the second dimension, the four types of tocopherols (α, β, γ, δ) are separated. The repeatability of the standard samples achieved a high reproducibility with RSD < 0.1%. Additionally, measurements conducted on multiple vegetable oils showed that quantification was possible without the influence of interfering peaks. The single-loop heart-cut 2D-LC method is the simplest technique among 2D-LC methods, making it applicable for routine analysis and a useful tool for food analysis involving samples with a high content of matrix components. 【Features】 ■ High-precision separation achieved with the single-loop heart-cut 2D-LC method ■ Separation of β-tocopherol and γ-tocopherol, which is difficult in reverse-phase systems ■ Effective separation of interfering substances in food ■ High reproducibility with repeatability of RSD < 0.1%

• Analytical Equipment and Devices

The Thermo Scientific Vanquish Duo UHPLC system is a dual LC system equipped with two independent flow paths, allowing for parallel condition testing of different separation chemistries, such as the combination of ion exchange chromatography (IEX) and ion-pair reverse phase chromatography (IP-RP), within the same system. This technical document utilizes a method scouting kit to examine various ion-pairing agents, gradient conditions, pH, and column temperatures, achieving good peak shapes for mRNA and high-sensitivity detection of impurities. By simultaneously investigating IEX and IP-RP, it is possible to understand the separation characteristics of the sample, enabling efficient determination of separation conditions. With high separation capability and sensitivity, impurities can be effectively detected during post-transcription purification, allowing for reliable mRNA purification. **Features** - Simultaneous examination of two different separation chemistries (IEX and IP-RP) using the same device. - High-sensitivity detection of impurities in post-transcription purification through mRNA profiling. - Efficient determination of suitable separation conditions using the method scouting kit. - Examination of up to 10 solvents per column type to determine appropriate mobile phase conditions.

• Analytical Equipment and Devices

Quantitative analysis of gloss agents (saccharin and 2-butin-1,4-diol) in electroplating nickel plating solution using LC-MS helps maintain a consistent composition of the plating solution and greatly aids in the quality control of gloss agents. This method analyzes the components contained in the gloss agents under two different conditions based on their chemical composition. Additionally, samples can be easily measured by simply diluting them with water, and good results ranging from 92% to 108% have been obtained in addition-recovery tests. Furthermore, analytical conditions have been devised to achieve stable measurements over a long period. By using MS, detection is possible even at low concentrations in samples containing high concentrations of impurities, such as plating solutions, and detection is possible with both UV and MS. Measurement of not only gloss agents but also decomposition and by-products is possible, leading to further evaluation of the plating solution. For more details, please download the materials. 【Features】 ■ Quality control of gloss agents achieved through quantitative analysis using LC-MS ■ Measurement of gloss agents and decomposition/by-products is possible ■ Analysis of gloss agent components under two different conditions ■ Samples can be easily measured by simply diluting with water ■ Analytical conditions enable stable measurements over a long period

• Analytical Equipment and Devices

This technical document presents a case study on the highly sensitive quantification of eight components, including monosaccharides, disaccharides, and sugar alcohols, in beverages using the Thermo Scientific ISQ EC single quadrupole mass spectrometer. There are various methods for sugar analysis using HPLC, but traditional differential refractive index detectors have challenges such as sensitivity issues and the inability to perform gradient analysis. Additionally, while fluorescence detectors can detect low concentrations, they require derivatization, making the sample preparation complicated. The ISQ EC single quadrupole mass spectrometer enables the quantification of sugars at low concentrations ranging from a few to several tens of ng/mL, thanks to its high sensitivity and selectivity. Furthermore, its user-friendly device and analysis software allow easy operation for both beginners and experts in mass spectrometry, streamlining routine analyses and enabling the rapid acquisition of reliable quantitative data. 【Features】 ■ High sensitivity: Detection limit of a few to several tens of ng/mL ■ High selectivity: Accurate quantification unaffected by interfering components ■ Improved operability: No complex sample preparation required

• Analytical Equipment and Devices

This technical document presents a case study of the simultaneous analysis of polyphenols contained in beverages and health foods with high sensitivity, unaffected by other components. A 1.5 µm solid-core particle column was used for the analysis, separating 36 types of polyphenols under gradient conditions within 10 minutes. By using standard samples of each polyphenol, calibration curves and detection limits were confirmed, achieving good linearity with a coefficient of determination of over 0.997 and detection limits in the low ng/mL range. Additionally, in the spiking recovery tests conducted on multiple health foods and beverages, approximately 100% recovery was obtained, confirming accurate quantification without interference from food components. This system reliably separates and detects components with similar structures, such as polyphenols, providing reduced analysis time and high reproducibility. Furthermore, its compact design and ease of operation streamline routine analyses. 【Features】 ■ Simultaneous analysis of 36 polyphenol components ■ High separation using a 1.5 µm solid-core particle column ■ Detection limits in the low ng/mL range and high sensitivity ■ Good calibration curves with a coefficient of determination over 0.997 ■ Accurate quantification with approximately 100% recovery, unaffected by food components

• Analytical Equipment and Devices

This technical document introduces a post-column analysis system for accurately quantifying organic acids in various beverages. The analysis column uses ion exclusion mode, and by adding BTB solution post-column, it separates and detects ten types of organic acids with good reproducibility. The e-workflow feature, which supports operations in routine analysis, is a significant advantage as it fully automates the process from device startup to measurement and report generation. The HPLC system features a compact design that incorporates a dual pump with two pumps in one module for analysis and post-column solution delivery. Additionally, even in the event of an error, the automatic switching of valves prevents the post-column reaction solution from backflowing into the analysis column, allowing for safe adoption in routine analysis. 【Features】 ■ Accurate quantification of ten types of organic acids with good reproducibility of less than 1% ■ Separation of organic acids using ion exclusion mode columns, allowing measurement without matrix effects ■ Detection limits for organic acids in this method range from a few to 7 μg/mL ■ Automation of the process from device startup to report generation with the e-workflow feature ■ Ensured safety with automatic valve switching in case of errors

• Analytical Equipment and Devices