<Currently Available Webinar> About RNAscope in situ Hybridization Services for In Vivo Distribution in Gene Therapy

This concerns the visual detection and quantification of in vivo distribution of AAV and LV vectors and the expression of introduced genes in preclinical animal models using RNAscope and BaseScope in situ hybridization.
RNAscope in situ hybridization (ISH) is described by users as a means for "morphology-based analysis of AAV in vivo distribution." The RNAscope in situ assay for AAV DNA and introduced gene mRNA combines the molecular sensitivity of qPCR with single-cell resolution and the context of tissue morphology.
BaseScope ISH is another powerful tool that facilitates the development of gene therapies. Specific measurements of therapeutic human introduced gene expression in animal models are often impossible by IHC due to the high level of homology between the introduced gene and animal ortholog transcripts. BaseScope allows for specific detection even of single nucleotide differences, enabling easy distinction of AAV human introduced genes from non-human primate and other animal ortholog sequences.

Date and time | Always open |
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Entry fee | Free |
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