Highly sensitive measurement of carbonylated proteins! Reagents for oxidative stress research!
The "Carbonylated Protein Measurement Kit" is a marker for protein oxidative damage in humans and animals. Traditionally, colorimetric methods and HPLC methods utilizing the reaction with dinitrophenylhydrazine (DNPH) have been used to measure carbonylated proteins, but there have been issues such as the need for a large sample volume. This kit allows for the high-sensitivity measurement of carbonylated proteins by reacting the carbonylated proteins in the sample with DNPH and then using a specific antibody that binds to the DNPH-conjugated proteins. 【Features】 ■ Reacts carbonylated proteins in the sample with DNPH ■ Uses a specific antibody for DNPH-conjugated proteins ■ Measures carbonylated proteins *For more details, please refer to the PDF document or feel free to contact us.
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【Specifications】 ■Measurement targets: Plasma, bronchoalveolar lavage fluid, cerebrospinal fluid, cell extracts, etc. derived from humans and animals (excluding rabbit-derived samples) ■Measurement range: 156 - 2500 pmol/mg protein ■Time required: Approximately 2 hours for pretreatment and about 4 hours for ELISA measurement (or overnight + 1 hour) ■Sample volume required: Approximately 4L ■Number of tests: 96 wells ■Required equipment: ・Microplate reader (measurement wavelength 450nm) ・8-channel micropipette (50–250μL) ・Micropipette (for 0.5–1000μL) ・Eppendorf tubes (1.5mL) ・Distilled water ・Centrifuge ・Vortex mixer ・Ultrafiltration filter (Product code: KPC-FLT) ・Protein concentration measurement kit (Product code: KPC-BCA, etc.) ■Storage conditions: Refrigerated (2–8°C; do not freeze) *For more details, please refer to the PDF document or feel free to contact us.
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For more details, please refer to the PDF document or feel free to contact us.
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Nikken Zail Co., Ltd. is a company that provides research reagents in a wide range of fields, including medicine, pharmacy, biochemistry, and food. Among them, the Japan Institute of Aging Control develops, manufactures, and sells research reagents in areas such as anti-aging, and also conducts contract testing. We will continue to enhance our research and development and production technology, while providing new products and services to contribute to the health of people around the world.
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