Pre-treatment for the analysis of desmopressin in serum using LC-MS/MS with EVOLUTE EXPRESS WCX.
This application note describes a sample preparation protocol for the LC-MS/MS detection of the peptide desmopressin in human serum using solid-phase extraction (SPE). Desmopressin is an antidiuretic hormone and a synthetic vasopressin analog. It is clinically used for the treatment of nocturnal enuresis and diabetes insipidus, as well as for coagulation disorders. The method used here achieves reproducible and high recovery rates for desmopressin peptides in serum while minimizing co-elution of proteins, lipids, and phospholipids. The extraction from serum was performed using the EVOLUTE EXPRESS WCX96 well plate. EVOLUTE EXPRESS SPE products provide a robust and efficient high-throughput automated extraction solution. *You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.
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*You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.
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*You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.
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Biotage Japan Co., Ltd. is a wholly-owned subsidiary of Biotage AB, which is headquartered in Uppsala, Sweden (BIOT: NASDAQ/OMX Stockholm). Biotage AB has its roots in several venture companies that originated from Uppsala University, a leading science and engineering university in the Nordic region. After undergoing international acquisitions and restructuring throughout the 2000s, it has recently achieved remarkable growth as a global company.