By selecting plates, resins, fibers, etc. that combine with the foster tag, separation is possible depending on the number of phosphate groups!
The "Phos-tag technology" is a groundbreaking method for the separation, purification, and detection of phosphorylated substances using Phos-tag. Phos-tag is a functional molecule developed by the Laboratory of Pharmaceutical Molecular Function Science at Hiroshima University, which captures phosphate monoester anions (R-OPO32-) at neutral pH (physiological pH). Our company offers contract analysis using Phos-tag SDS-PAGE, providing strong support for research on protein phosphorylation. Additionally, we synthesize special Phos-tag compounds that have undergone chemical modifications such as various labeling, immobilization to single entities, and amino acid introduction. 【Features of Phos-tag SDS-PAGE】 ■ Enables analysis of substrate protein phosphorylation by kinases ■ Quantitatively visualizes phosphorylated and non-phosphorylated substrates ■ Analyzes the phosphorylation state of proteins within cells ■ Allows for Western blot analysis of target proteins ■ Bands can be excised from the gel after staining for mass spectrometry analysis *For more details, please refer to the related link page or feel free to contact us.
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【Flow from Request for Analysis to Data Delivery】 ■Consultation ↓ ■Detailed Meeting ↓ ■Sending of Samples ↓ ■Preliminary Inspection ↓ ■Phos-tag SDS-PAGE Analysis *For more details, please refer to the related link page or feel free to contact us.
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【Purpose】 ■ Capping the phosphate groups of phosphorylated compounds to utilize the spin difference and mass difference with comparative samples through nuclear magnetic resonance and mass spectrometry for the detection of unknown donors. ■ By adding the charge possessed by the foster group, electrical changes and mass changes occur, which can be utilized in electrophoresis and chromatography, etc. *For more details, please refer to the related link page or feel free to contact us.
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Nerd Research Institute is a contract synthesis company that supports research and development. We custom synthesize compounds that are not commercially available and assist research and development in a wide range of fields through chemical synthesis. In our business style, highly specialized researchers directly listen to the voices of our customers, share the challenges that need to be addressed, and propose solutions while progressing with the work. If you are having trouble with obtaining compounds or industrialization processes, please feel free to contact us. We broadly support contract synthesis for pharmaceuticals, cosmetics, pesticides, OLED materials, automotive materials, battery materials, medical device materials, bio-related reagents, regenerative medicine, and biomaterials.