analysis Product List and Ranking from 309 Manufacturers, Suppliers and Companies

Contract analysis and commissioned analysis of CAE - Frequency response analysis

Experienced engineers will propose appropriate analysis methods tailored to the customer's analysis objectives. We will assist with analyses seeking steady-state responses using simple sine waves.

Frequency response analysis is used to confirm vibration issues in products. It can help identify problems caused by vibrations and contribute to improvements in safety and quality. It is utilized in various fields such as automotive, aerospace, railways, shipping, plants, industrial machinery, construction machinery, civil engineering, and the medical sector. 【Features】 - Proposal-based contract analysis by experienced engineers. - Suggestions for condition setting considerations and improvement measures based on analysis results. - Thorough pursuit of "high quality," "short delivery times," and "cost performance." - The software used includes globally trusted and high-precision tools like 'ABAQUS' and 'NASTRAN.' - High repeat rate. 【Examples of Target Products】 - Automobiles - Aircraft - Railways - Ships - Plant equipment (pressure vessels, piping, etc.) - Construction machinery - Machine tools and presses - Molds and jigs - Semiconductor devices - Robotic arms - Industrial equipment - Bridges and dams - Medical devices and nursing equipment It is utilized in various areas. For more details, please request materials or view the PDF data available for download.

Company：ダイコーテクノ
Price：Other

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Contract analysis and commissioned analysis of CAE: Spectral response analysis

Experienced engineers will propose appropriate analysis methods tailored to your analysis objectives. We will assist you in evaluating the maximum response using seismic waves.

Spectral response analysis is used to identify vibration issues in products. It can help confirm problems caused by vibrations and contribute to improvements in safety and quality. It is utilized in various fields such as automotive, aerospace, railways, shipping, plants, industrial machinery, construction machinery, civil engineering, and the medical sector. 【Features】 - Proposal-based contract analysis by experienced engineers. - Suggestions for condition setting considerations and improvement measures based on analysis results. - Thorough pursuit of "high quality," "short delivery times," and "cost performance." - The software used includes globally trusted and high-precision tools like 'ABAQUS' and 'NASTRAN.' - High repeat rate. 【Examples of Target Products】 - Automobiles - Aircraft - Railways - Ships - Plant equipment (pressure vessels, piping, etc.) - Construction machinery - Machine tools and presses - Molds and jigs - Semiconductor devices - Robot arms - Industrial equipment - Bridges and dams - Medical devices and nursing equipment It is applied in various areas. For more details, please request materials or view the PDF data available for download.

Company：ダイコーテクノ
Price：Other

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CAE Contract Analysis, Commissioned Analysis, and Undertaking - Thermal Analysis

Experienced engineers will propose appropriate analysis methods tailored to your analysis objectives. We will help you confirm temperature distribution and thermal stress, and assist with countermeasures and considerations.

Thermal analysis is used to identify thermal issues in products. It helps to confirm problems caused by heat and contributes to improvements in safety and quality. It is utilized in various fields such as automotive, aerospace, railways, shipping, plants, industrial machinery, construction machinery, civil engineering, and the medical field. 【Features】 - Proposal-based contract analysis by experienced engineers. - Suggestions for condition setting considerations and improvement measures based on analysis results. - Thorough pursuit of "high quality," "short delivery times," and "cost performance." - The software used includes globally trusted and high-precision tools like 'ABAQUS' and 'NASTRAN.' - High repeat rate. 【Examples of Target Products】 - Automobiles - Aircraft - Railways - Ships - Plant equipment (pressure vessels, piping, etc.) - Construction machinery - Machine tools and presses - Molds and jigs - Semiconductor devices - Robot arms - Industrial equipment - Bridges and dams - Medical devices and nursing equipment It is utilized in various areas. For more details, please request materials or view the PDF data available for download.

Company：ダイコーテクノ
Price：Other

Contract Analysis
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Structural Analysis
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CAE Contract Analysis, Commissioned Analysis, and Outsourcing - Thermal Stress Analysis

Experienced engineers will propose appropriate analysis methods tailored to your analysis objectives. We will help you confirm the effects of thermal stress and assist with countermeasures and considerations.

Thermal stress analysis is used to identify thermal issues in products. It can help confirm problems caused by heat and contribute to improvements in safety and quality. It is utilized in various fields such as automotive, aerospace, railways, shipping, plants, industrial machinery, construction machinery, civil engineering, and the medical sector. 【Features】 - Proposal-based contract analysis by experienced engineers. - Suggestions for condition setting considerations and improvement measures based on analysis results. - Thorough pursuit of "high quality," "short delivery times," and "cost performance." - The software used includes globally trusted and high-precision tools like 'ABAQUS' and 'NASTRAN.' - High repeat rate. 【Examples of Target Products】 - Automobiles - Aircraft - Railways - Ships - Plant equipment (pressure vessels, piping, etc.) - Construction machinery - Machine tools and presses - Molds and jigs - Semiconductor devices - Robot arms - Industrial equipment - Bridges and dams - Medical devices and nursing equipment It is utilized in various areas. For more details, please request materials or view the PDF data available for download.

Company：ダイコーテクノ
Price：Other

Contract Analysis
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Structural Analysis
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Contract analysis and commissioned analysis of CAE: Thermal conduction analysis

Experienced engineers will propose appropriate analysis methods tailored to your analysis objectives. We will help you confirm the temperature distribution state and assist with countermeasures and considerations.

In thermal conduction analysis, it is used to identify thermal issues in products. It can help confirm problems caused by heat and contribute to improvements in safety and quality. It is utilized in various fields such as automotive, aerospace, railways, shipping, plants, industrial machinery, construction machinery, civil engineering, and the medical field. **Features** - Proposal-based contract analysis by experienced engineers. - Suggestions for condition setting considerations and improvement measures based on analysis results. - Thorough pursuit of "high quality," "short delivery times," and "cost performance." - The software used includes globally trusted and high-precision tools like 'ABAQUS' and 'NASTRAN.' - High repeat rate. **Examples of Target Products** - Automobiles - Aircraft - Railways - Ships - Plant equipment (pressure vessels, piping, etc.) - Construction machinery - Machine tools and presses - Molds and jigs - Semiconductor devices - Robotic arms - Industrial equipment - Bridges and dams - Medical devices and nursing equipment It is utilized in various areas. For more details, please request materials or view the PDF data available for download.

Company：ダイコーテクノ
Price：Other

Contract Analysis
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Structural Analysis
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CAE Contract Analysis, Commissioned Analysis, and Outsourcing - Buckling Analysis

Experienced engineers will propose appropriate analysis methods tailored to your analysis objectives. We will assist with analyses such as confirming buckling modes.

Buckling analysis is used to confirm buckling issues in products. It helps to identify what kind of buckling modes may occur, contributing to safety verification and quality improvement. It is utilized in various fields such as automotive, aerospace, railways, shipping, plants, industrial machinery, construction machinery, civil engineering, and the medical field. **Features** - Proposal-based contract analysis by experienced engineers. - Suggestions for condition setting considerations and improvement measures based on analysis results. - Thorough pursuit of "high quality," "short delivery times," and "cost performance." - Use of globally trusted and high-precision software such as 'ABAQUS' and 'NASTRAN.' - High repeat rate. **Examples of Target Products** - Automobiles - Aircraft - Railways - Ships - Plant equipment (pressure vessels, piping, etc.) - Construction machinery - Machine tools and presses - Molds and jigs - Semiconductor devices - Robot arms - Industrial equipment - Bridges and dams - Medical devices and nursing equipment It is utilized in various areas. For more details, please request materials or view the PDF data available for download.

Company：ダイコーテクノ
Price：Other

Contract Analysis
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CAE Contract Analysis, Commissioned Analysis, and Outsourcing - Collision Analysis

Experienced engineers will propose appropriate analysis methods tailored to your analysis objectives. We assist in analyzing phenomena related to the collision of objects.

In collision analysis, it is used to identify collision issues in products. It helps to confirm what kind of forces occur during a collision, what behaviors are exhibited, and can be utilized for safety verification and quality improvement. It is utilized in various fields such as automotive, aerospace, railways, shipping, plants, industrial machinery, construction machinery, civil engineering, and the medical field. 【Features】 - Proposal-based contracted analysis by experienced engineers. - Suggestions for condition setting considerations and improvement measures based on analysis results. - Thorough pursuit of "high quality," "short delivery times," and "cost performance." - The software used includes globally trusted and high-precision tools like "Radioss." - High repeat rate. 【Examples of Target Products】 - Automobiles - Aircraft - Railways - Ships - Plant equipment (pressure vessels, piping, etc.) - Construction machinery - Machine tools and presses - Molds and jigs - Semiconductor devices - Robot arms - Industrial equipment - Bridges and dams - Medical devices and nursing equipment It is utilized in various areas. For more details, please request materials or view the PDF data from the download section.

Company：ダイコーテクノ
Price：Other

Contract Analysis
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CAE Contract Analysis, Commissioned Analysis, and Outsourcing - Drop Analysis

Experienced engineers will propose appropriate analysis methods tailored to your analysis objectives. We will assist you in analyzing the phenomenon of objects falling and colliding.

Drop analysis is used to verify drop issues in products. It helps to confirm what kind of forces occur during a drop, what behaviors are exhibited, and can be utilized for safety verification and quality improvement. It is utilized in various fields such as automotive, aerospace, railways, shipping, plants, industrial machinery, construction machinery, civil engineering, and the medical field. **Features** - Proposal-based contract analysis by experienced engineers. - Suggestions for condition setting considerations and improvement measures based on analysis results. - Thorough pursuit of "high quality," "short delivery times," and "cost performance." - Utilizes globally trusted and high-precision software such as "Radioss." - High repeat rate. **Examples of Target Products** - Automobiles - Aircraft - Railways - Ships - Plant equipment (pressure vessels, piping, etc.) - Construction machinery - Machine tools and presses - Molds and jigs - Semiconductor devices - Robot arms - Industrial equipment - Bridges and dams - Medical devices and nursing equipment It is utilized in various areas. For more details, please request materials or view the PDF data available for download.

Company：ダイコーテクノ
Price：Other

Contract Analysis
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Thermal Fluid Analysis

Visualization through thermal fluid simulation

Utilizing 3D data, a model is created. Temperature distribution and fluid simulations are conducted. By visualizing, it becomes even easier to imagine.

Company：グローバル本社
Price：Other

Thermo-fluid analysis
analysis

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ASU/V-Struct Release Analysis Edition

Predicting and solving product behavior during demolding in resin molding! By identifying the causes of demolding defects and taking preventive measures, we reduce design changes and renovation costs.

This is the "ASU/V-Struct Release Analysis Edition," customized specifically for release analysis during resin injection molding. It is software designed to identify the causes of release defects during injection molding and to implement countermeasures in advance, thereby improving the quality of molded products and reducing the costs associated with mold design changes and modifications. - It enables the prediction of sticking and cavity capture in advance, identifying areas that will be captured by the cavity based on displacement distribution and node force distribution. - It allows for the prediction of whitening and warping due to protrusion arrangement balance, identifying areas of deformation in molded products based on the evaluation of protrusion loads for each protrusion. - It can accommodate inclined cores (upward and downward) and diagonal movement of slide units. - It enables high-precision predictions that inherit the temperature distribution during injection molding (using ASU/MOLD).

Company：先端力学シミュレーション研究所
Price：Other

Structural Analysis
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Press molding and mold deformation weakly coupled analysis ASU/ISTR

This is an introduction to press forming simulation that takes into account mold deflection using "ASU/ISTR" and "ASU/P-form."

In the current press forming simulation, the mold is treated as a rigid body, and deflection is not taken into account. However, during actual press processing, especially when forming high-strength steel plates, a very large load is applied, resulting in mold deflection. This causes changes in the clearance between the upper and lower molds, leading to variations in contact and surface pressure on the workpiece depending on the location. Additionally, the amount of deflection varies based on the structure of the mold. To reproduce the phenomena of actual press processing in press forming simulation, an analysis that considers mold deflection is necessary. We will introduce a case of forming simulation that takes mold deflection into account using the open-source structural analysis software FrontISTR for mold deformation and our solver ASU/P-form for weakly coupled analysis. ASU/ISTR is a general-purpose pre- and post-processing software developed by our company that is compatible with FrontISTR. By utilizing the deflection weakly coupled setting function of ASU/ISTR, we can quickly incorporate the analysis results from ASU/P-form and automatically set the conditions. Furthermore, it allows for easy analysis setup and execution on a Windows PC. FrontISTR excels in parallel computing, and there are no licensing costs even when using a PC with a high number of cores.

Company：先端力学シミュレーション研究所
Price：Other

simulator
analysis

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Case Study Analysis: "Scuttle Shake Analysis of Open Cars by OMA"

Scuttle shake analysis of open cars using OMA (Operational Modal Analysis).

We would like to introduce a case study by F-MA Consulting, a specialist in vibration noise analysis technology, titled "Scuttle Shake Analysis of Open Cars Using OMA (Operational Modal Analysis)." The definition of scuttle shake is: "a phenomenon commonly seen in open cars with low body rigidity," "which manifests as a 'shaking sensation' when driving in urban areas at speeds of 40 to 60 km/h," and "is generally said to be caused by low-frequency (10 to 20 Hz) twisting modes of the body." 【Experimental Analysis Using ModalVIEW】 - Collection of acceleration and deceleration data from actual vehicle runs at each measurement point - Pole extraction using SSI from time history data - Calculation of mode shapes at each point from the extracted modes - Display of vibration modes in the operational state For more details, please contact us or download the catalog.

Company：F-MAコンサルティング
Price：Other

Other analyses
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[Data] Real Number Mode Curve Fitting Method and Its Applications

Analyzing the white body of passenger cars and pickup trucks! A comparison of the real mode method and the complex mode method was conducted.

The theory of curve fitting in experimental modal analysis has a long history during which various methods have been developed, and it is even said that there are no further mathematical advancements to be made. In this document, we introduce a real-valued modal curve fitting method that advances the assumption of real modes by using only the imaginary part of the FRF (or the real part when the FRF is in terms of velocity/force). Additionally, we applied this method to the white body of a passenger car and a pickup truck, and we present a comparative analysis of the results obtained from the real mode method and the complex mode method. We invite you to read it. [Contents] ■ Preface ■ Real Mode Method ■ Case Study of Passenger Car White Body ■ Case Study of Pickup Truck ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

Company：F-MAコンサルティング
Price：Other

Other analyses
others
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Practical Modal Analysis for Automobiles

We are publishing information about OMA (Operational Modal Analysis) and EMA (Experimental Modal Analysis)!

This document reports on cases of OMA (Operational Modal Analysis) using multi-point simultaneous measurements on SUV test vehicles, followed by cases of OMA and EMA (Experimental Modal Analysis) using multi-point non-simultaneous measurements on convertible car test vehicles. As a practical modal analysis for automobiles using a relatively small-scale system, OMA proposes a method to determine natural frequencies and damping ratios using SSI (Stochastic Subspace Identification), and to obtain mode shapes using ODS (Operational Deflection Shape). Additionally, EMA proposes a combination of sinusoidal excitation using a small electromagnetic shaker and the least squares complex frequency method. 【Contents】 ■ Preface ■ OMA (Operational Modal Analysis) ■ EMA (Experimental Modal Analysis) ■ Discussion ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

Company：F-MAコンサルティング
Price：Other

Other analyses
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Operational Vibration Shape Analysis "ODS Project"

It is possible to perform detailed structural analysis of dynamic characteristics! Clear user interface.

The "ODS Project" is part of the TPA Project's structural analysis package, which identifies vibration patterns using measured data and a measurement point library, and animates them under specified operational conditions for actual operational vibration shape analysis. It allows for a centralized overview of all information, automatically performs analysis and animation, and enables immediate evaluation. Additionally, it is possible to animate and analyze vibration shapes in unsteady structures using the Time Domain Animation Project (TDA). 【Main Features】 ■ Auto spectrum that displays selected channels on a color band and 2D diagram for analysis ■ FFT diagram for additional recorded air propagation channels ■ Shape Table for saving and selecting shapes to be animated ■ Automatic calculation of MPC values (mode phase co-linearity) *For more details, please refer to the PDF document or feel free to contact us.

Company：ヘッドアコースティクスジャパン
Price：Other

Structural Analysis
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Temperature distribution analysis

By using thermal analysis, it is possible to create high-precision heat plates and heaters. Please contact us if the temperature tolerance conditions are strict.

By conducting thermal analysis, it is possible to create high-precision heat plates. By conducting thermal analysis, it is possible to reduce the number of prototypes, thereby achieving cost reduction.

Company：新日電熱工業
Price：Other

Mold Design
analysis

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Real-time analysis of surveillance camera footage for quick response.

An image analysis AI that utilizes existing camera footage as is to achieve advanced image analysis.

SATLYS is a video analysis AI service provided by Toshiba Digital Solutions Corporation. It analyzes camera footage used for security, monitoring, and caregiving purposes in real-time, supporting the efficiency of on-site operations and quick decision-making. Since it can utilize existing surveillance camera footage as is, AI implementation can begin without the need to introduce new recording equipment. This service is valuable for manufacturers who want to add AI capabilities to their surveillance camera products and related systems, allowing them to enhance added value in a short period.

Company：光アルファクス
Price：Other

Image analysis software
Other embedded systems (software and hardware)
analysis

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Evaluation and analysis of product discoloration and other issues.

We will clarify the cause of discoloration based on information and analysis from our customers! Here, we introduce the analysis of product discoloration and other related issues.

Discoloration and stains on metal and plastic products can be very noticeable and may lead to defects. The causes can be due to the influence of chemical substances or extremely small adhering particles. One method to investigate these causes is to analyze the actual discoloration or stains, which can reveal the reason for the discoloration. Our company conducts investigations into the causes of discoloration based on information from customers and the results of our analysis. If you are experiencing issues with product defects, please feel free to consult us. 【Analysis Process】 ■ Information Gathering ■ Observation ■ Analysis ■ Reporting Results *For more details, please refer to the PDF document or feel free to contact us.

Company：環境アシスト
Price：Other

Contract Analysis
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Anisotropic design and analysis of CFRP

Would you like to develop high-performance CFRP parts with excellent cost performance?

At Tech Lab Co., Ltd., we offer "Anisotropic Design and Analysis of CFRP." We possess a variety of anisotropic material data necessary for anisotropic analysis of many PAN-based and pitch-based CFRPs. Through numerical analysis using FEM (Finite Element Method), we conduct optimal orientation and lamination design tailored to the specifications of parts and products. Furthermore, we propose feasible and cost-effective lamination configurations that take moldability into account, rather than just simple numerical analysis. [What can be achieved with anisotropic design (excerpt)] ■ Ultra-lightweight materials with a specific gravity of 1.5 to 1.7 that have high thermal conductivity ■ Parts with high thermal dimensional stability that have an ultra-low thermal expansion coefficient ■ Heat transfer/insulation that can be controlled based on direction ■ Ability to balance strength, rigidity, and thermal properties, among others *For more details, please refer to the PDF materials or feel free to contact us.

Company：テックラボ開発センター
Price：Other

Contract Analysis
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Contracted Sequence Analysis "Rental Analysis Service"

Available for request from 1run! A rental analysis service that can provide quick result reports.

The "Contracted Sequence Analysis Rental Analysis Service" is designed for customers who want to request only electrophoresis when "common equipment is crowded," who are "performing sequence reactions under special conditions," or who want to conduct electrophoresis of sequence reaction products more cheaply and quickly. Additionally, we offer the "Contracted Sequence Analysis Premix2 Shortened Delivery Time," which achieves speed and low cost by having customers prepare samples in each container. 【Features】 ■ Rental Analysis - Accepts requests from 1 run, allowing for differentiation based on sample length - Delivery the next business day after sample receipt (as fast as same day!) ■ Semi-Rental Analysis - Recommended for customers who do not want to perform labor-intensive purifications like ethanol precipitation - Delivery the next business day after sample receipt *For more details, please contact us or download the catalog.

Company：ファスマック
Price：Other

Contract Analysis
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Environmental DNA analysis

Solving your 'things you want to investigate' with DNA analysis technology! Would you like to apply it to testing and research?

We would like to introduce Fasmac's "Environmental DNA Analysis." In "DNA Metabarcoding using Next-Generation Sequencers," we utilize DNA barcoding regions to analyze diversity in environmental samples. We also accommodate unique target sequences from our customers. Customers can prepare the library themselves, and we will handle everything from sequencing to analysis. Additionally, we offer "Species-Specific Analysis by Quantitative PCR," which can be used for evaluating the presence or absence of organisms and estimating biomass. 【Features】 < DNA Metabarcoding using Next-Generation Sequencers > ■ Analyzing diversity in environmental samples using DNA barcoding regions ■ Free provision of primers for library preparation ■ Support for unique target sequences from customers ■ Possible to handle everything from DNA extraction to library preparation *For more details, please refer to the PDF document or feel free to contact us.

Company：ファスマック
Price：Other

Contract Analysis
Contract Analysis
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Microbial Typing Service (NGS MLST Analysis Service)

Rapid and high precision! Introducing a method for simultaneously identifying seven genetic regions and typing bacteria. *Case study collection available.

At Fasmac, we offer a "Microbial Typing Service (NGS MLST Analysis Service)." "MLST analysis" is a method of typing bacteria by patterning mutations in multiple gene regions (usually more than 7 regions), providing detailed strain information that cannot be distinguished by 16S rRNA gene analysis. The "MLST analysis" using NGS identifies seven gene regions simultaneously by mapping sequence data obtained from shotgun genome sequencing to the genomes of closely related species, and it is believed to be faster and more accurate compared to traditional methods. We have added microbial typing analysis to Fasmac's next-generation sequencing analysis services. Please feel free to contact us if you would like to make an inquiry. *For more details, please refer to the PDF document or feel free to contact us.

Company：ファスマック
Price：Other

Contract Analysis
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RNA-seq analysis *Case study collection available

From library preparation to data analysis! Introducing FastMac's RNA-seq analysis!

At Fasmac, we offer "RNA-seq analysis." "RNA-seq" allows for comprehensive quantification of all transcripts expressed within cells by analyzing mRNA using NGS. To quantify the expression levels of all transcripts, RNA, which is the transcript from the sample, is first extracted and fragmented. Then, cDNA libraries are created from the fragmented RNA, followed by sequencing. Additionally, we provide a variety of custom services that allow you to choose the analysis content according to your experimental plan. [Analysis Overview] ■ (Customer) Provide sample information ■ Send samples to us ■ Library preparation ■ Sequence analysis ■ Data analysis *For more details, please refer to the PDF document or feel free to contact us.

Company：ファスマック
Price：Other

Contract Analysis
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Analysis of live microbial community structure *Case study collection available

Introducing new amplicon analyses from next-generation sequencing! We present two approaches.

At Fasmac, we offer "Analysis of Live Microbial Community Structure." The "Community Structure Analysis using the Selective Membrane Permeable Dye EMA" utilizes the fact that EMA modifies DNA derived from dead bacteria, rendering the modified DNA unable to be amplified by PCR, allowing for the selective detection of DNA from live bacteria. Additionally, the "Community Structure Analysis using cDNA from Reverse Transcription of RNA" is a method for identifying actively functioning microorganisms by analyzing RNA in environmental samples. 【Features】 <Community Structure Analysis using Selective Membrane Permeable Dye EMA> ■ Utilizes the modification of dead bacterial DNA by EMA, which prevents PCR amplification of the modified DNA ■ Selectively detects DNA from live bacteria <Community Structure Analysis using cDNA from Reverse Transcription of RNA> ■ Analyzes RNA in environmental samples ■ Identifies actively functioning microorganisms *For more details, please refer to the PDF document or feel free to contact us.

Company：ファスマック
Price：Other

Contract Analysis
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Genotyping Analysis: PCR-Electrophoresis Analysis

Detection of larger indels and screening using HMA! Introducing our genotyping analysis.

At Fasmac, we offer "PCR-Electrophoresis Analysis." We can detect band shifts (10bp and above) due to base deletions/inserts and perform HMA (heteroduplex mobility assay) analysis. The analysis equipment used is the "PerkinElmer LabChip GX Touch HT," and the samples include tissue fragments from mice, rats, zebrafish, and medaka, as well as cultured cell pellets. Please feel free to contact us when you need our services. 【Analysis Details】 ■ Delivery Time: 1 to 2 weeks ■ Samples: Tissue fragments from mice, rats, zebrafish, and medaka, cultured cell pellets, etc. ■ Deliverables: Electrophoresis images ■ Analysis Equipment: PerkinElmer LabChip GX Touch HT *For more details, please refer to the PDF document or feel free to contact us.

Company：ファスマック
Price：Other

Contract Analysis
Contract Analysis
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Antibody sequence analysis

We can also accept optional work! We will support the production of recombinant antibodies.

At Fasmac, we offer "Antibody Sequence Analysis." By obtaining the variable sequences of antibodies, we support the production of recombinant antibodies. We amplify the variable sequences of mouse IgG genes expressed in hybridomas using the 5' RACE method or RT-PCR, perform DNA cloning in E. coli, and conduct sequence analysis. Additionally, we can accommodate other optional tasks depending on the situation. Please feel free to consult with us. 【Analysis Details】 ■ Provided Material: Hybridoma total RNA 1μg (at least 100ng/μl) or cell pellet 1×10^6 cells ■ Deliverables: Report, sequence analysis data (Data for 10 clones each of H chain and L chain will be reported) ■ Delivery Time: 2 to 3 weeks *For more details, please refer to the PDF document or feel free to contact us.

Company：ファスマック
Price：Other

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[Next-Generation Sequencing Analysis Case] MLST Analysis *Case study collection available

We will introduce a case study that was able to identify strains mixed in environmental DNA!

MLST (Multi-locus sequencing typing) analysis is a method for typing bacteria by patterning mutations in multiple gene regions (usually more than seven). In this analysis case (in-house data), samples were prepared assuming the identification of isolates' genomes and environmental bacteria (samples mixed with environmental DNA and the genomes of isolated strains), and typing was conducted. The prepared strains included Gram-positive and Gram-negative bacteria, and genome sequencing and typing were performed on these samples using Miseq. As a result, this method allowed for the identification of strains mixed with environmental DNA. [Case Overview] - Samples were prepared assuming the identification of isolates' genomes and environmental bacteria, and typing was conducted. - The prepared strains were Bacillus subtilis subsp. subtilis str. 168 (Gram-positive) and Pseudomonas protegens Pf-5 (Gram-negative). - Genome sequencing and typing were performed on these samples using Miseq. *For more details, please refer to the PDF document or feel free to contact us.*

Company：ファスマック
Price：Other

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[Next-Generation Sequencing Analysis Case] 12S Amplicon Analysis

We present a case that suggests PCR repetition is effective in increasing the number of fish species detected! *Case study collection available.

We would like to introduce an example analyzed using MiFish, which is used for DNA metabarcoding of fish. Water samples were collected from multiple locations in the Sagami River system and pooled in a plastic tank. To prevent DNA degradation, 1 ml/L of Osban disinfectant was added immediately after sampling. The samples were brought back at room temperature, and filtration and DNA extraction were performed in the lab. The obtained data was provided to MitoFish for homology search. As a result, it was observed that the number of fish species detected increased, suggesting that PCR repetition is effective in increasing the number of detected fish species. [Case Overview] - Filtration and DNA extraction were performed in the lab. - NGS library preparation was conducted using the extracted DNA. - Library preparation was performed in 8 repetitions. - The prepared library was sequenced using Illumina's MiSeq. - The obtained data was provided to MitoFish for homology search. *For more details, please refer to the PDF document or feel free to contact us.*

Company：ファスマック
Price：Other

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[Next-Generation Sequencing Analysis Case] COI Amplicon Analysis

We have found that it is an effective means to elucidate the relationship between hosts and parasites that is difficult to detect from appearance! *Case study collection is being distributed.

At the Kyoto University Center for Ecological Research, research is being conducted on the diversification of the genus Acer and the processes involved in coexistence among species. Our company has been commissioned to conduct part of the analysis, which we would like to introduce. In this analysis, we utilized host-specific blocking primers to suppress the amplification of host-derived genes, allowing for the efficient identification of the host, the leafroller moth, and a comprehensive exploration of parasites within it. We found that the analytical method using blocking primers is an effective means of elucidating the relationships between hosts and parasites that are difficult to detect visually. 【Case Overview】 ■ PCR was conducted in the presence and absence of blocking primers. ■ COI amplicon analysis was performed on 274 samples of leafroller moth larvae using Miseq. ■ From the obtained reads, we identified 10 species of leafroller moths and 13 species of parasitic wasps, clarifying the host-parasite relationships. *For more details, please refer to the PDF document or feel free to contact us.

Company：ファスマック
Price：Other

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[Case Study] Determination of Insertion Sites for Animal Cultured Cells and Plant Cells

We have added a case study of random integration analysis using the RAISING method!

We would like to introduce a case study of our random integration analysis service using the RAIS method. We performed the RAIS method on T-DNA taglines inserted into Arabidopsis thaliana using the Agrobacterium method, as well as on mouse cultured cells with retrovirus vectors inserted. We confirmed the insertion site sequences using Sanger sequencing and mapped the decoded sequences obtained from next-generation sequencing analysis to the genome sequence. As a result, it was confirmed that this method is effective for determining insertion sites in animal cultured cells as well as in plant cells. 【Random Integration Analysis Service using the RAIS Method】 ■ Sanger Sequencing Plan ■ Next-Generation Sequencing (NGS) Plan * For more details, please refer to the PDF document or feel free to contact us. * For case studies, please refer to the URL below.

Company：ファスマック
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