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Contracted Sequence Analysis "Rental Analysis Service"

Available for request from 1run! A rental analysis service that can provide quick result reports.

The "Contracted Sequence Analysis Rental Analysis Service" is designed for customers who want to request only electrophoresis when "common equipment is crowded," who are "performing sequence reactions under special conditions," or who want to conduct electrophoresis of sequence reaction products more cheaply and quickly. Additionally, we offer the "Contracted Sequence Analysis Premix2 Shortened Delivery Time," which achieves speed and low cost by having customers prepare samples in each container. 【Features】 ■ Rental Analysis - Accepts requests from 1 run, allowing for differentiation based on sample length - Delivery the next business day after sample receipt (as fast as same day!) ■ Semi-Rental Analysis - Recommended for customers who do not want to perform labor-intensive purifications like ethanol precipitation - Delivery the next business day after sample receipt *For more details, please contact us or download the catalog.

  • Contract Analysis

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Environmental DNA analysis

Solving your 'things you want to investigate' with DNA analysis technology! Would you like to apply it to testing and research?

We would like to introduce Fasmac's "Environmental DNA Analysis." In "DNA Metabarcoding using Next-Generation Sequencers," we utilize DNA barcoding regions to analyze diversity in environmental samples. We also accommodate unique target sequences from our customers. Customers can prepare the library themselves, and we will handle everything from sequencing to analysis. Additionally, we offer "Species-Specific Analysis by Quantitative PCR," which can be used for evaluating the presence or absence of organisms and estimating biomass. 【Features】 < DNA Metabarcoding using Next-Generation Sequencers > ■ Analyzing diversity in environmental samples using DNA barcoding regions ■ Free provision of primers for library preparation ■ Support for unique target sequences from customers ■ Possible to handle everything from DNA extraction to library preparation *For more details, please refer to the PDF document or feel free to contact us.

  • Contract Analysis
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  • Analysis Services

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Genotyping Analysis: PCR-Electrophoresis Analysis

Detection of larger indels and screening using HMA! Introducing our genotyping analysis.

At Fasmac, we offer "PCR-Electrophoresis Analysis." We can detect band shifts (10bp and above) due to base deletions/inserts and perform HMA (heteroduplex mobility assay) analysis. The analysis equipment used is the "PerkinElmer LabChip GX Touch HT," and the samples include tissue fragments from mice, rats, zebrafish, and medaka, as well as cultured cell pellets. Please feel free to contact us when you need our services. 【Analysis Details】 ■ Delivery Time: 1 to 2 weeks ■ Samples: Tissue fragments from mice, rats, zebrafish, and medaka, cultured cell pellets, etc. ■ Deliverables: Electrophoresis images ■ Analysis Equipment: PerkinElmer LabChip GX Touch HT *For more details, please refer to the PDF document or feel free to contact us.

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  • Analysis Services

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Antibody sequence analysis

We can also accept optional work! We will support the production of recombinant antibodies.

At Fasmac, we offer "Antibody Sequence Analysis." By obtaining the variable sequences of antibodies, we support the production of recombinant antibodies. We amplify the variable sequences of mouse IgG genes expressed in hybridomas using the 5' RACE method or RT-PCR, perform DNA cloning in E. coli, and conduct sequence analysis. Additionally, we can accommodate other optional tasks depending on the situation. Please feel free to consult with us. 【Analysis Details】 ■ Provided Material: Hybridoma total RNA 1μg (at least 100ng/μl) or cell pellet 1×10^6 cells ■ Deliverables: Report, sequence analysis data (Data for 10 clones each of H chain and L chain will be reported) ■ Delivery Time: 2 to 3 weeks *For more details, please refer to the PDF document or feel free to contact us.

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  • Analysis Services

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Next-generation sequencing analysis "Microbial Genome Draft Analysis Package"

Samples can accept bacteria and archaea! *Case study collection is currently being distributed.

The "Microbial Genome Draft Analysis Package" is a service that can be used reasonably by being packaged. Acceptable samples include bacteria and archaea. As optional services, we offer auto-annotation using Prokka, BLAST analysis of contigs, and sequence type typing. Please feel free to contact us when you need our services. 【Analysis Overview】 (1) Customer: Send the extracted genomic DNA to us. (2) Our company: Perform QC work on the received samples. (3) Our company: Prepare the adapter library for MiSeq analysis. (4) Our company: Conduct a 2×300 bp run on MiSeq. (5) Our company: Assemble the acquired data and create contigs. *For more details, please refer to the PDF document or feel free to contact us.

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Next-generation sequencing analysis "Targeted amplicon analysis"

We offer a variety of optional services! Data is visualized as "microbial community composition graphs" and "heat maps." *Case study collection available.

"Target Amplicon Analysis" is a service that can be used reasonably by packaging it. The target regions for analysis include 16S V4, 18S V9, ITS (gITS7-ITS4), COI (mlCOIintF-HCO2198), and MiFish (12S V5), among others. We provide primers for sample preparation targeting the amplification regions free of charge, and you will send us your samples. After that, we will use QIIME and other tools to visualize the data as "microbial community composition graphs" and "heat maps." [Optional Services (Excerpt)] - Change of target region length (approximately 400-500 bp) - Library mix preparation - Rarefaction curve and principal coordinate analysis (PCoA) - OTU BLAST analysis *For more details, please refer to the PDF document or feel free to contact us.

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DNA random integration analysis

Short time, simple, high sensitivity, low-cost DNA insertion site analysis.

It is possible to determine the insertion sites of foreign DNA randomly integrated into the host genome, including the target location. This can be used to identify insertion sites of viral genome, viral vector, fusion genes that may serve as drug discovery targets, and off-target knock-ins in genome editing. Compared to traditional methods for determining transgene insertion sites such as Tail-PCR, LAM-PCR, and Southern blotting, this approach enables analysis that is quicker, simpler, more sensitive, and lower in cost.

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Genotyping analysis

PCR-electrophoresis analysis and PCR-NGS analysis! Detection using real-time PCR and digital PCR is also possible.

To everyone who is worried about 'having to do genotyping but not having enough time,' leave the labor-intensive tasks to Fasmac. In our 'Genotyping Analysis,' we perform DNA extraction, PCR, and mutation detection. Please send us tissue samples from mice or zebrafish, cultured cell pellets, etc. We will provide a quote based on the number of samples and the analysis you require. Additionally, detection using real-time PCR and digital PCR is also possible. 【Line-up】 ■ PCR-Electrophoresis Analysis: Detection of larger indels and screening using HMA ■ PCR-Sanger Seq Analysis: Detection of small indels and single nucleotide substitutions ■ PCR-NGS Analysis: Detailed analysis of mosaic mutations *For more details, please refer to the PDF document or feel free to contact us.

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  • Analysis Services

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Genotyping Analysis: PCR-Sanger Sequencing Analysis

Detection of small indels and single nucleotide substitutions! Introducing our genotyping analysis.

At Fasmac, we offer "PCR-Sanger Seq analysis." With Sanger sequencing, we can confirm the presence of single nucleotide substitutions and the number of nucleotide deletions/inserts that cannot be detected by electrophoresis analysis. Samples can include tissue fragments from mice, rats, zebrafish, and medaka, as well as cultured cell pellets, and the deliverables will be sequencing analysis data. Please feel free to contact us when you need our services. 【Analysis Details】 ■ Delivery Time: 1-2 weeks ■ Samples: Tissue fragments from mice, rats, zebrafish, and medaka, cultured cell pellets, etc. ■ Deliverables: Sequencing analysis data ■ Analysis Equipment: Applied Biosystems 3730xl DNA Analyzer *For more details, please refer to the PDF document or feel free to contact us.

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Genotyping Analysis: PCR-NGS Analysis

Detailed analysis of mosaic mutations! Introducing genotyping analysis using Illumina MiSeq.

At Fasmac, we offer "PCR-NGS analysis." We conduct detailed analysis of the types and frequencies of mutations using next-generation sequencers. The analysis device used is the "Illumina MiSeq," which allows for the simultaneous analysis of one target site (up to 16 samples). For details regarding pricing, delivery times, and more, please feel free to contact us. 【Analysis Details】 ■ Simultaneous analysis of 16 samples for one target site ■ Example: Knockout in cultured cells ■ Analysis device: Illumina MiSeq *For more information, please refer to the PDF document or feel free to contact us.

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Fragment analysis

Currently, it is widely used as a DNA testing method and DNA variety identification method under development!

"Fragment analysis" involves customers sending PCR products amplified using fluorescent primers, which are then assessed for fragment length through capillary electrophoresis by a sequencer. Our company conducts fragment analysis using a capillary sequencer to assist with DNA variety testing and technological development. We also aim to provide proposals tailored to our customers' needs, so please feel free to contact us. 【Features of DNA Sequencing Service】 - Analysis conducted domestically allows for detailed and prompt responses. - All data is visually checked by specialized staff. - The sequencer has undergone official maintenance by the manufacturer. - Re-electrophoresis is performed on samples expected to show improved data. *For more details, please refer to the PDF document or feel free to contact us.*

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