Contract for the production of genetically modified mice: Creation of transgenic mice.
Tissue samples are taken from the obtained offspring! They are produced by microinjection into fertilized eggs.
Our company is engaged in the production of "transgenic mice." Transgenic mice are created by purifying DNA fragments of the expression unit that includes a promoter, the gene to be expressed, and a polyA sequence from plasmid DNA, and then microinjecting them into fertilized eggs. From the resulting offspring, tissue samples are collected, and genotyping PCR is performed to investigate the insertion of the injected gene into the genome, thereby identifying the founder mouse (FO). [Features] - Production by injecting the gene expression unit into fertilized eggs of the C57BL/6 strain - Forced expression of the gene in mouse individuals - Specific expression can be achieved by selecting the promoter - Strong expression throughout the body is also possible by using the CAG promoter *For more details, please refer to the PDF materials or feel free to contact us.
- Company:トランスジェニック
- Price:Other