メルク株式会社ライフサイエンス（シグマ アルドリッチ ジャパン合同会社） Product Lineup

"Benzonase Salt Tolerant Endonuclease Emprove Expert" is a newly developed enzyme. It is designed using advanced protein engineering to exhibit the highest level of activity even under high salt concentration conditions. This protein is a monomer with a molecular weight of approximately 27 kDa and an isoelectric point (pI) of pH 9.68. It functions at pH 6-10 and 0-37°C. 【Features】 ■ A new endonuclease designed for gene therapy manufacturing ■ Shows activity under high salt concentration conditions (up to 1M NaCl) ■ Non-animal derived, recombinant from E. Coli, high purity ■ Compliant with GMP (IPEC-PQG) and Emprove Expert, FDA DMF ■ Free from glycosylation and post-translational modifications ■ Accurate detection using a proprietary InnoM assay *For more details, please download the PDF or feel free to contact us.

The "Cellvento 4CHO-X Expansion Medium" has been specially developed for N-1 perfusion of Chinese hamster ovary (CHO) cell lines. It contains high concentrations of nutrients to achieve high cell density at a low CSPR (Cell Specific Perfusion Rate: the amount of medium supplied per cell). It is chemically defined, free from animal-derived components, and does not contain any undefined components such as hydrolysates. 【Features】 ■ Specially formulated to control the metabolic profile of cells ■ Minimizes toxic by-products ■ Ensures appropriate nutrient levels for high cell density ■ Prevents nutrient depletion, improving the robustness and predictability of the seed train process ■ Usable in all expansion steps of the seed train, including N-1 ■ Achieves robust proliferation at low CSPR *For more details, please download the PDF or feel free to contact us.

"Cellvento ModiFeed Prime COMP" is a chemically defined (CD) medium that replenishes depleted nutrients necessary for cell function and enables high-concentration single feed at neutral pH to extend the production culture period in fed-batch mode. It is formulated for fed-batch production of monoclonal antibodies (mAb) and biopharmaceuticals using various Chinese hamster ovary (CHO) cell expression systems. It can be used in combination with Cellvento 4CHO COMP and EX-CELL Advanced CHO production media or many major basic media for CHO cells. 【Features】 ■ All components are non-animal origin (NAO) and do not contain hydrolysates, phenol red, or 2-mercaptoethanol ■ High concentration formulation (>131g/L) ■ Formulation without glucose ■ Achieves single feed containing modified amino acids and amino acid derivatives *For more details, please download the PDF or feel free to contact us.

"Cellvento 4CHO" is a chemically defined cell culture medium. It has been developed for the proliferation of Chinese hamster ovary (CHO) cells in suspension culture, as well as for the production of monoclonal antibodies and recombinant proteins. It is chemically defined, free from animal-derived components, and does not contain any undefined components such as hydrolysates. Additionally, it does not use L-glutamine. The feed contains both cysteine and tyrosine, eliminating the need for additional alkaline feed. 【Features】 ■ To be widely usable even with cells that have the dhfr gene introduced, it does not contain hypoxanthine or thymidine. ■ To allow fine-tuning of glucose concentration in fed-batch processes, the feed does not contain glucose, which helps keep lactate production low. *For more details, please download the PDF or feel free to contact us.

"Cellvento 4HEK Medium" is a chemically defined medium optimized for the production of adeno-associated virus (AAV) in various human embryonic kidney (HEK) 293 cell lines. It is streamlined and includes components that enhance medium stability and suppress protein oxidation. The composition does not contain any animal-derived components. It can be used as a cell amplification and vector production medium for gene therapy vector manufacturing. 【Features】 - Compatible with commonly used transfection reagents such as PEI - Facilitates AAV production, making it easier to obtain high-titer full capsids in various HEK293 cell lines - Usable at all stages including proliferation, transfection, and production - Suitable for research or manufacturing purposes - Can be used immediately after taking it out of the refrigerator without returning it to room temperature *For more details, please download the PDF or feel free to contact us.

"Cellvento ModiFeed Gal-COMP" is a chemically defined (CD) feed that contains only non-animal-derived components. It is used in the development and manufacturing of monoclonal antibodies (mAb) and biopharmaceuticals based on a Chinese hamster ovary (CHO) cell expression system. To enhance usability, the product is supplied as a compaction-dried powder medium, making it convenient to handle and easy to dissolve. 【Features】 - Complete feed medium that minimizes galactosylation - As a CD feed free from animal-derived components, it allows for easier process management and reduces complexity - The compaction feed reduces the complexities of dissolution, storage, and addition - The dissolved feed is stable at room temperature, eliminating the need for temperature cycling during the process (refrigerated management and warming before feeding) *For more details, please download the PDF or feel free to contact us.

"Cellvento ModiFeed Gal＋ COMP" is a chemically defined (CD) feed that contains only non-animal derived components. It is used in the development and manufacturing of monoclonal antibodies (mAbs) and biopharmaceuticals in a Chinese hamster ovary (CHO) cell-based expression system. To enhance usability, the product is supplied as a compacted dry powder medium, making it convenient to handle and easy to dissolve. 【Features】 ■ A complete feed medium that increases terminal galactose in various CHO cell-based expression systems. ■ As a CD feed free from animal-derived components, it allows for easier process management and reduces complexity. ■ The compacted feed reduces the complexity of dissolution, storage, and addition. *For more details, please download the PDF or feel free to contact us.

"Cellvento ModiFeed Prime COMP" is a chemically defined (CD) feed that contains only non-animal-derived components. It does not contain hydrolysates, phenol red, 2-mercaptoethanol, or glucose, enhancing uniformity between batches. It is intended for use in the development and manufacturing of monoclonal antibodies and biopharmaceuticals using a Chinese hamster ovary (CHO) cell expression system. 【Features】 - Added to replenish depleted nutrients necessary for cell function and extend the culture period in fed-batch mode. - Single feed at high concentration and neutral pH. - Due to its high concentration exceeding 131g/L, the volume of the additive to the medium is reduced, improving the yield of the target product. - Specially designed to enhance process management and reduce complexity. *For more details, please download the PDF or feel free to contact us.

"Cellvento ModiFeed Sial+ COMP" is a chemically defined (CD) feed that contains only non-animal-derived components. It is used for the development and manufacturing of monoclonal antibodies (mAb) and biopharmaceuticals based on a Chinese hamster ovary (CHO) cell expression system. To enhance usability, the product is supplied as a compaction dry powder medium, making it convenient to handle and easy to dissolve. 【Features】 ■ A complete feed medium that maximizes sialylation ■ As a CD feed free of animal-derived components, it allows for easier process management and reduces complexity ■ The compaction feed reduces the complexity of dissolution, storage, and addition ■ The dissolved feed is stable at room temperature, eliminating the need for temperature cycling during the process (refrigerated management and warming before feeding) *For more details, please download the PDF or feel free to contact us.

The "CHOZN Elite Cell Line" is a CHO (Chinese Hamster Ovary) mammalian cell expression system that proliferates in a suspension culture using a chemically defined medium free of animal-derived components. It is a subclone of the CHOZN GS KO cell line, achieving performance that surpasses the parent strain in various performance indicators such as efficiency of cell line development (CLD), predictable growth, and protein production levels. A technical user guide detailing the procedures for optimizing performance will be provided along with the cell line. **Features** - High-producing clones with titers of 7 to 13 g/L can be obtained through the introduction of standard vectors, resulting in cost reduction. - Easy to adopt and can be directly integrated into the customer's existing CLD processes, compatible with both fed-batch and perfusion processes. *For more details, please download the PDF or feel free to contact us.*

"Cellvento, EX-CELL CHO cell culture media and feed media" is a comprehensive commercial product that supports all production processes and cell line development needs for Chinese hamster ovary (CHO) cells. It is optimized for the production of high-performance monoclonal antibodies and recombinant proteins in fed-batch, enhanced fed-batch, and perfusion cultures. Advanced technologies such as modified amino acids and media compaction are incorporated into the catalog media portfolio to meet the needs for process enhancement. [Related Products] ■ CHOZN GS Cell Line ■ Millipore Express Filters ■ Mobius Breez Microbioreactor ■ Cellicon Assembly ■ Mobius HCDC Assembly ■ Mobius iFlex Bioreactor *For more details, please download the PDF or feel free to contact us.

We would like to introduce the "Deviron Surfactants" that we handle. In response to the global impact of the usage restrictions on "Triton X-100," we have developed two surfactants: 'Deviron C16 Emprove Evolve' and 'Deviron 13-S9 Emprove Expert.' Please check the downloadable materials for a comparison table that will help you evaluate and select products. 【Deviron C16 Emprove Evolve Specifications (Partial)】 ■ Surfactant Type: Amphoteric (pI 8.9) ■ Chemical Name: N,N-Dimethyl-tetradecylamine-N-oxide ■ Critical Micelle Concentration (CMC): 0.002-0.003% wt (24°C) ■ Composition: 30% (w/v) aqueous solution ■ Packaging: 1L, 2.5L, 25L *For more details, please download the PDF or feel free to contact us.

"EX-CELL Advanced CHO Fed-Batch Medium" is a chemically defined medium. It was developed using multivariate analysis of over 10,000 data points, including specifications for performance, physical properties, regulatory compliance, and safety design. It is designed to be used in conjunction with "EX-CELL Advanced CHO Feed 1" to achieve excellent platform performance in fed-batch culture of all commercial production CHO cell lines (CHO-S, DuxB11, DG44, CHO-M, and CHOZN GS). [Features] - A product intended for manufacturing applications in the biomanufacturing industry - Chemically defined - Free from animal-derived components - Free from L-glutamine, hypoxanthine, and thymidine *For more details, please download the PDF or feel free to contact us.

We have developed a high-purity NTP EmproveExpert portfolio that complies with IPEC-PQG GMP standards to ensure consistency between batches in your mRNA manufacturing process. These critical raw materials come with comprehensive Emprove dossiers. We provide essential regulatory support to appropriately address the complex regulatory requirements of the biopharmaceutical industry, helping ensure compliance with regulations and reducing time to market. 【Features】 ■ Manufactured in compliance with IPEC-PQG GMP, improving consistency between batches ■ Free from animal-derived components (AOF) ■ High purity (over 99%) ■ Microbial and endotoxin specifications set at low levels ■ Nuclease activity specifications set below the detection limit ■ Residual solvent specifications set at low levels *For more details, please download the PDF or feel free to contact us.

This document is a handbook regarding raw materials for biopharmaceutical processes. It provides a comprehensive overview of our process raw materials and additives in upstream, downstream, and formulation processes, along with detailed information on regulatory background and regulatory support. We understand our customers' needs for safety, security, scalability, and process efficiency, enabling us to deliver therapeutic solutions to patients more quickly. [Contents (partial)] ■ Bioprocess: Proposals for process raw materials in biopharmaceutical manufacturing ■ Recent topics on pharmaceutical additives and process raw materials ■ Regulatory support for bioprocess raw materials ■ Upstream and cell culture biopharmaceutical process raw materials ■ Process raw materials for buffers, downstream, and formulation ■ Solid process raw materials for buffers and bioprocesses *For more details, please download the PDF or feel free to contact us.

This document discusses the optimization of feed strategies for enhanced fed-batch processes using "Cellvento ModiFeed Prime Feed." It introduces a study examining the effects of starting the feed from the first day of culture, rather than on the third day as in traditional fed-batch processes, to achieve successful cultivation at high cell densities. Additionally, various feed schedules have been evaluated to optimize cultivation performance. [Contents (partial)] ■ Future prospects of enhanced fed-batch ■ Impact of first-day feed on medium formulation ■ Importance of first-day feed in enhanced batch cultivation ■ Evaluation of feed schedules ■ Control of cultivation environment *For more details, please download the PDF or feel free to contact us.