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■ High-speed analysis column "TSKgel FcR-IIIA-NPR" - Capable of recognizing differences in the N-linked glycan structures of the antibody Fc region and separating antibodies with different ADCC activities. - Liganded with recombinant human Fcγ receptor IIIa, offering improved stability compared to the native type. - Allows for direct separation of antibody samples without the need for purification or pre-treatment. - Separation can be achieved in a short time (20 minutes). (Including equilibration time, measurements can be made in 30 minutes per cycle.) ■ Preparative column "TSKgel FcR-IIIA-5PW" - Demonstrates equivalent separation selectivity as it uses the same ligand as TSKgel FcR-IIIA-NPR. - Capable of loading approximately 5 mg of antibody sample. - Allows for the collection of the amount necessary for structural analysis and activity measurement in a single preparative chromatography run. *For more details, please refer to the PDF document or feel free to contact us.*

The identification of critical quality attributes (CQAs) of antibody drugs, the establishment of their management scope, and their analysis can be effectively achieved using HPLC/UHPLC. We will introduce the TSKgel series (columns) used in the analysis of critical quality attributes and risk assessment of antibody drugs with UHPLC/HPLC.

The "TSKgel(R) UP-SW3000-LS" is a size exclusion chromatography column for the separation of biomacromolecules, using a filler with a particle size of 2μm that has diol groups chemically bonded to the surface of silica gel. Due to the use of fine particle fillers (2μm), it enables rapid analysis (15cm column) and high-resolution analysis (30cm) of biomacromolecules such as proteins (antibodies, enzymes). It has minimal column shedding, making it suitable for measurements using light scattering detectors. 【Features】 ■ The surface characteristics of the filler are similar to the existing TSKgel SW type. ■ Minimal column shedding makes it ideal for measurements using light scattering detectors. ■ Improved filler and column materials reduce initial protein adsorption. ■ Shipping and storage solvents are easily managed with a 20% ethanol aqueous solution. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.

This document is a technical material that explains the high-performance SEC column TSKgel UP-SW3000. We will introduce the basic characteristics and separation examples. [Contents (excerpt)] ■ Introduction ■ Basic characteristics of TSKgel UP-SW3000 ■ Application examples ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

● The surface properties of the packing material are similar to the existing TSKgel SW type. ● It is suitable for the separation of low molecular weight proteins, fragments, peptides, and oligonucleotides. 【Features】 ■ Rapid analysis (15 cm column) and high-resolution analysis (30 cm) are possible. ■ The surface properties of the packing material are similar to the existing TSKgel SW type. ■ Optimized for the separation of antibody (IgG) dimers, monomers, and fragments. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.*

"TSKgel Protein C4-300" is a reverse phase chromatography column for the separation of biomacromolecules, using a packing material with silica gel of particle size 3μm and pore size 30nm, with butyl groups (C4) immobilized. It exhibits strong protein retention and excellent separation selectivity. 【Features】 ■ Strong protein retention and excellent separation selectivity ■ High recovery rate for general water-soluble proteins, with superior peak shape ■ Due to the use of fine particle packing material (3μm), it enables high throughput, high separation, and high sensitivity measurements ■ The 2mm inner diameter column is suitable for LC/MS (/MS) measurements *For more details, please refer to the PDF document or feel free to contact us.

"TSKgel Protein A-5PW" is an affinity chromatography column filled with a recombinant Protein A introduced into a porous hydrophilic polymer substrate. The substrate with a particle size of 20μm has high mechanical strength, allowing for high-speed analysis of IgG. It has a high IgG adsorption capacity and a wide quantification range. Additionally, the column material uses PEEK, resulting in low nonspecific adsorption. 【Features】 ■ The substrate with a particle size of 20μm has high mechanical strength, enabling high-speed analysis of IgG. ■ It has a high IgG adsorption capacity and a wide quantification range. ■ Durable column. ■ It has the same ligand as the separation and purification carrier TOYOPEARL AF-rProtein A HC-650F, providing similar separation selectivity. *For more details, please refer to the PDF document or feel free to contact us.

This document explains size exclusion chromatography (SEC) for HPLC columns. It includes features of SEC columns and a list of compatible H-type solvents. Additionally, the section on SEC column selection provides a detailed explanation of both aqueous SEC column selection (for synthetic polymers) and organic solvent-based SEC column selection, using tables for clarity. 【Contents (excerpt)】 ■ Features of SEC columns ■ List of compatible H-type solvents ■ SEC column selection ■ Aqueous SEC columns (for proteins, peptides, and nucleic acids) ■ Aqueous SEC columns (for synthetic polymers, polysaccharides, oligosaccharides, and nucleic acids) *For more details, please refer to the PDF document or feel free to contact us.

This document explains the IEC, HIC, and affinity of HPLC columns. It introduces the characteristics of ion exchange chromatography columns, as well as basic physical properties. Additionally, it explains each product series using tables. Furthermore, it also includes information on hydrophobic chromatography and affinity chromatography. 【Contents (excerpt)】 ■ Ion Exchange Chromatography ■ Hydrophobic Chromatography ■ Affinity Chromatography *For more details, please refer to the PDF document or feel free to contact us.

This document is the product catalog for TOYOPEARL and TSKgel series. In "Process Chromatography for Antibodies," we present the characteristics of various chromatography separation modes, as well as key TOYOPEARL products for antibody purification, using tables. Furthermore, the product list categorizes numerous products under each item. [Contents (Excerpt)] <TOYOPEARL> ■ Process Chromatography for Antibodies Characteristics of various chromatography separation modes Key TOYOPEARL products for antibody purification Protein A and Protein L carriers Ion exchange carriers ■ Product List (Process Chromatography) *For more details, please refer to the PDF document or feel free to contact us.

This document is a technical material that explains liquid chromatography. Liquid chromatography has become an essential method for the separation and analysis of biological samples. This guide provides a comprehensive explanation of various separation modes of liquid chromatography, their principles, and features, ranging from the basics to actual measurements. [Contents (excerpt)] - Properties of proteins and nucleic acids - Separation methods for biopolymers - Liquid chromatographs used for measuring biopolymers - Size exclusion chromatography - Ion exchange chromatography *For more details, please refer to the PDF document or feel free to contact us.

This document is a technical material explaining the TSKgel STAT series columns for high-performance ion exchange chromatography. It introduces the basic characteristics and application examples. [Contents] ■ Introduction ■ Basic properties of the column - Characteristics of the packing material - Column pressure - Separation of standard proteins - Separation of nucleic acids - Sample loading amount ■ Application examples of anion exchange columns ■ Application examples of cation exchange columns ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

This document is a technical material explaining the TSKgel Protein C4-300, a high-performance reversed-phase chromatography column for the separation of biomacromolecules. It introduces the basic properties, the impact of measurement conditions on separation, comparisons with other RPC columns, and examples of separations. 【Contents (excerpt)】 ■ Introduction ■ Features ■ Basic Properties ■ Separation Examples ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

This document is a technical material explaining the high-performance normal phase chromatography (NPC) / hydrophilic interaction chromatography (HILIC) column TSKgel Amide-80 2μm. We introduce the TSKgel Amide-80 2μm, which improves separation performance compared to the conventional TSKgel Amide-80 by reducing the particle size to 2μm. [Contents (excerpt)] ■ Introduction ■ Features ■ Basic Properties ■ Separation Examples ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

This document introduces the high-performance AFC column "TSKgel FcR-IIIA-NPR" handled by Tosoh Corporation. It includes the basic properties of the product, standard separation conditions, application examples, and usage precautions. The new measurement method using this column allows for very simple and quick results with good reproducibility. Additionally, this column can be used not only for quality control of antibody drugs but also for screening production cell lines, optimizing cell culture medium composition, and process analysis of culture processes. 【Contents (partial)】 ■ Introduction ■ Basic Properties - Properties of the packing material - Standard separation conditions - Effects of eluent composition - Effects of sample load amount - Durability - Cleaning methods - Lot-to-lot variation of packing material - Storage stability, etc. *For more details, please refer to the PDF document or feel free to contact us.

This document is a technical material explaining the high-performance affinity chromatography column TSKgel Protein A-5PW. It introduces basic properties, features, and separation examples. [Contents (excerpt)] ■ Introduction ■ Basic properties ■ Separation examples ・ High-speed analysis ・ Quantification of monoclonal IgG (spike recovery test) ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

This document is a technical material that explains the high-performance SEC column TSKgel UP-SW2000. We will introduce its basic characteristics and separation examples. 【Contents (excerpt)】 ■ Introduction ■ Basic characteristics of TSKgel UP-SW2000 ■ Application examples ・ Separation of biopharmaceuticals ・ Separation of oligonucleotides ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

This document is a technical material explaining the high-performance SEC column TSKgel UP-SW Aggregate. We will introduce its basic characteristics and separation examples. 【Contents (excerpt)】 ■ Introduction ■ Basic characteristics of TSKgel UP-SW Aggregate ■ Analysis examples - Analysis of heat-treated antibody aggregates - Analysis of IgM - Analysis of nucleic acids ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

● The surface properties of the packing material are similar to the existing TSKgel SW type. ● Ideal for the separation of high molecular weight aggregates of antibodies, high molecular weight proteins, and nucleic acid fragments. ■ Quick analysis (15 cm column) and high-resolution analysis (30 cm) are possible. ■ The surface properties of the packing material are similar to the existing TSKgel SW type. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.

Optimized microparticle fillers (2µm, 3µm) enable rapid analysis (15cm column) and high-resolution analysis (30cm) of biomacromolecules such as proteins, antibodies, and enzymes. The surface characteristics of the fillers are similar to those of the widely used general-purpose SWXL series columns. They are compatible with both UHPLC and HPLC systems.

The latest column is the UP-SW3000-LS. Please refer to that as well. The "TSKgel UP-SW3000" is a size exclusion chromatography column for the separation of biomacromolecules, using a packing material that chemically bonds diol groups to the surface of silica gel particles with a diameter of 2μm. Due to the fine particle packing material, rapid analysis (15cm column) and high-resolution analysis (30cm) of biomacromolecules such as proteins, antibodies, and enzymes are possible. 【Features】 ■ Enables rapid analysis (15cm column) and high-resolution analysis (30cm) ■ Surface characteristics of the packing material are similar to existing TSKgel SW types ■ Optimized for the separation of antibody (IgG) dimers, monomers, and fragments ■ Compatible with both UHPLC and HPLC systems *For more details, please refer to the PDF document or feel free to contact us.

The latest column is UP-SW3000-LS. Please refer to that as well. This document is a technical material that explains the high-performance SEC column TSKgel UP-SW3000. We will introduce the basic characteristics and examples of separation. [Contents (excerpt)] ■ Introduction ■ Basic characteristics of TSKgel UP-SW3000 ■ Application examples - Precise analysis of antibody drugs - Separation example of antibody papain digests ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

We will introduce examples of oligonucleotide separation using hydrophilic interaction chromatography (HILIC) and ion-pair reversed-phase chromatography.

This is a technical document explaining the features of the new TOSOH SEC column "TSKgel UP-SW3000-LS," which addresses the phenomenon of non-specific adsorption of proteins (initial adsorption) often observed when equilibrating a new size exclusion chromatography (SEC) column with elution buffer and injecting protein samples. This phenomenon is particularly prevalent with antibody samples, where aggregates or dimers may adsorb, leading to a reduction in elution peaks or, in some cases, no peaks being eluted at all. Such occurrences are seen in nearly all SEC columns.