JIS L 1921 (Test Method for Antifungal Properties of Textile Products)
This is a test to compare the inhibitory and suppressive effects on mold growth between antimicrobial-treated samples and untreated samples by inoculating the test samples with mold spore suspension, culturing them, and measuring the ATP levels before and after culturing. This can be conducted on absorbent samples such as fiber products and paper. ISO 13629-1 describes a similar testing method. *ATP is a substance synthesized by living bacteria. Arrangements for the testing method are also possible. If you would like to discuss changes to testing conditions, bacterial species, or sample characteristics, please feel free to consult with us.
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basic information
- Necessary information for conducting the test To provide a smooth estimate, it would be helpful to receive the following information: - Number of types of samples - Test microorganism species - Availability of samples corresponding to control samples - Required deliverables* (if there are any besides the Japanese report) - Method of sample preparation (if you wish to conduct the test using samples treated with liquids or wet wipes, etc.) - Any test conditions you wish to change (if applicable) *Unless there are specific requests, the report will consist solely of a Japanese report (including test methods and test results (results of test condition assessments, ATP levels, antifungal activity values), without images).
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Applications/Examples of results
**Testing Method** 1. Preparation of Test Spore Solution According to the regulations, a 1/20 Sabouraud Dextrose liquid medium is used for preparation. Depending on the testing purpose, this nutritional condition may be modified. *A single spore solution is used. Unlike the mold resistance test, a mixed spore solution cannot be used.* 2. Preparation of Samples Prepare test samples and control samples cut to 0.20±0.03 g. *For each sample, three pieces are used before and after cultivation for testing.* *Sterilization of samples is not performed unless instructed otherwise.* *Control samples refer to "materials similar to the test samples that have not undergone anti-fungal processing."* *Additional notes regarding control samples are available.* *It is also possible to use test pieces that have undergone some treatment with liquids, etc., as test samples. If cotton cloth pieces are needed, we can prepare them.* 3. Inoculation of Test Spore Solution and Cultivation Inoculate the test spore solution onto the samples and cultivate at 25±2°C for 42±2 hours. 4. Measurement and Evaluation of ATP Levels - Using dedicated reagents, measure the ATP levels in the mold cells before and after cultivation. - Compare the ATP measurement results between the test samples and control samples to evaluate the anti-fungal effect.
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At our center, we not only conduct tests as specified by JIS and other regulations, but we also propose testing methods that suit the purpose of the tests. We capture the entire problem for problem-solving and provide practical advice.
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