AATCC TM100 (Test Method for Antibacterial Finishes on Textile Materials)
This is a testing standard from AATCC (American Association of Textile Chemists and Colorists). It quantitatively examines the antibacterial effect of treated textile products on the growth of bacteria. Please feel free to inquire about other AATCC standards (such as TM147).
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basic information
- Necessary information for conducting the test For a smooth estimation, it would be helpful to receive the following information: - Number of types of specimens - Whether there are preparations for unprocessed test pieces - Required deliverables* (if there are any besides the Japanese report) - Test microorganism species (if you want to conduct the test with species different from the specified ones) - Method of preparing test pieces (if you want to use test pieces treated with liquids or wet wipes for testing) *Unless there are specific requests, the report will only be in Japanese (including test methods and test results (results of determining test conditions, viable cell counts, Percent Reduction) without images).
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Applications/Examples of results
**Testing Method** 1. Preparation of Test Spore Solution Prepare using tryptone soy broth or nutrient medium diluted 20 times with a 0.05% polysorbate 80 aqueous solution. 2. Sample Preparation Cut the samples (antimicrobial-treated test pieces and untreated test pieces) into circles with a diameter of 4.8±0.1 cm or squares with a side of 3.8±0.1 cm, stacking them to a total weight of 1.0±0.1 g. *Although the testing document does not specify the number of repetitions, prepare three samples stacked to 1.0±0.1 g each and conduct the tests simultaneously.* 3. Inoculation and Cultivation of Test Spore Solution Inoculate the samples with 1.0±0.1 mL of the test bacterial solution, then place them in sterilized sealed containers and incubate at 37±2°C for 24 hours. 4. Inoculation and Cultivation of Test Bacterial Solution Immediately after inoculation and 24 hours after incubation, wash the samples with 100 mL of SCDLP liquid medium, and prepare a tenfold dilution series of the washout solution with purified water. After inoculating this onto standard agar medium (n=2), incubate at 37±2°C for 24 to 48 hours and count the colonies. 5. Evaluation Calculate the Percent Reduction (R) based on the regulations.
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At our center, we not only conduct tests as specified by JIS and other regulations, but we also propose testing methods that suit the purpose of the tests. We capture the entire problem for problem-solving and provide practical advice.
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