Method for Testing Antibacterial Activity of Household Synthetic Detergents and Soaps
Test method for antibacterial activity of synthetic detergents and soaps for residential use.
This is the testing method of the Detergent and Soap Fair Trade Council. After bringing the bacteria and the sample into contact through the test piece, the number of viable bacteria on the test piece is measured to investigate the disinfection effect.
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basic information
This is the testing method of the Detergent and Soap Fair Trade Council. It is a method to investigate the antibacterial effect by measuring the viable bacterial count on a test piece after exposing it to bacteria and the sample. The features are as follows: 1. The test piece is used. Bacteria and the sample are brought into contact through a circular stainless steel piece. There is also a method of disinfecting liquid agents that does not use a test piece, which is the most fundamental method. 2. Nutrients are added to the bacterial solution. Bovine serum albumin is added to the bacterial solution as a model soiling substance. When there is dirt, the effect on the bacteria in the sample tends to be less pronounced compared to when there is no dirt. * The Japanese Pharmacopoeia "Hard Surface Carrier Method" involves inoculating a bacterial solution without added nutrients onto a test piece (with no specified material) measuring approximately 5 cm x 5 cm and measuring the viable bacterial count on the test piece. If you would like this method, please consult us accordingly.
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Applications/Examples of results
**Testing Method** Each of the test samples and control samples will be repeated three times. 1. **Preparation of Test Bacterial Solution** Prepare a bacterial solution by mixing an equal amount of a solution adjusted to 10^8 CFU/mL using 1/2 nutrient broth with a 30 g/L bovine serum albumin aqueous solution. Depending on the testing purpose, the nutritional conditions can be modified. 2. **Preparation of Samples** Prepare as follows: - Test Sample: The original liquid of the specimen, or the specimen (liquid or powder) adjusted to the specified concentration (the expected usage concentration of the specimen or the concentration for disinfection appeal) using a solvent (as specified, hard water). - Control Sample: 0.05% (w/v) Polysorbate 80 aqueous solution. 3. **Inoculation of Test Bacterial Solution and Samples onto Test Pieces, Incubation** After inoculating the test bacterial solution onto stainless steel test pieces (20 mm in diameter), inoculate the samples and let them sit for 5 minutes. Although the standard specifies 5 minutes, this can be adjusted considering the desired effect duration. 4. **Measurement of Viable Bacterial Count** Wash out the test carriers and measure the viable bacterial count. 5. **Evaluation** Compare the viable bacterial counts obtained from testing the test samples and control samples using common logarithmic values, and calculate the disinfection activity value.
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At our center, we not only conduct tests as specified by JIS and other regulations, but we also propose testing methods that suit the purpose of the tests. We capture the entire problem for problem-solving and provide practical advice.
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