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column(hplc) - List of Manufacturers, Suppliers, Companies and Products

Last Updated： Aggregation Period:Dec 17, 2025～Jan 13, 2026
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column Product List

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Core-shell type filling column "SunShell"

Core-shell type filling column "SunShell"

Column back pressure halved, achieving high separation! A column that also achieves high durability.

"SunShell" is a core-shell type filling column that achieves efficient silanol group deactivation and high durability by using the improved Sunniest-bonding method for core-shell silica. The core-shell column exhibits equivalent performance (theoretical plates) at half the back pressure of a column with a particle size of 1.8µm. Due to the low back pressure, there are fewer failures in UHPLC even after prolonged use. 【Features】 - Core sizes of 1.6μm, 3.0μm, and 3.4μm with porous layers of 0.5μm, 0.2μm, and 0.6μm - 2.6μm offers the same number of plates as fully porous Sub2μm and 2μm, with the same column pressure as fully porous 3μm - 5μm has the same number of plates as fully porous 3μm and the same column pressure as fully porous 5μm - 3.4μm is a core-shell packing material for rapid protein separation - The reverse phase stationary phases such as C18 and C8 have the same surface treatment as Sunniest For more details, please contact us or download the catalog.

  • Other laboratory equipment and containers

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HPLC/UHPLC column "FlexFire C1"

HPLC/UHPLC column "FlexFire C1"

It is possible to shorten analysis time! It has surprising performance that enhances the retention of highly polar compounds to the maximum limit.

The first choice of columns in HPLC/UHPLC method development is almost always C18, and to change selectivity from C18, columns such as C30, C8, and HILIC are selected as second choices. The 'FlexFire C1' is a column that uses refined silica gel. The perception of "this product = low adsorption" holds unexpected performance that enhances peak shape and peak sensitivity, as well as maximizes the retention of highly polar compounds. 【Features】 ■ Contains unexpected performance that maximizes the retention of highly polar compounds ■ Significantly weakens excessively strong retention ■ Allows for reduced analysis time without changing the method ■ Utilizes refined silica gel *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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[Case Study] AAV Titer Measurement After Downstream Process

[Case Study] AAV Titer Measurement After Downstream Process

By adopting size exclusion chromatography and highly sensitive fluorescence detection, accurate and reproducible measurements can be achieved with a small amount of sample!

We introduce a rapid and cost-effective method for measuring the titer of adeno-associated virus (AAV) products after downstream processing, combining size exclusion chromatography (SEC) with a highly sensitive fluorescence detector. By using the Thermo Scientific MAbPac SEC-1 column, efficient separation of AAV capsids and low molecular weight impurities is achieved, allowing for detailed monitoring of product quality attributes (PQA). This method enables rapid determination of AAV product titer after a single step of affinity purification, contributing to the evaluation of product characteristics. 【Features】 ■ Rapid and cost-effective titer measurement method ■ Measurement possible with small sample sizes due to high-sensitivity fluorescence detection ■ Efficient separation using the MAbPac SEC-1 column ■ Detailed monitoring of PQA is possible ■ Improved reproducibility with low-adsorption glass vials (RSD less than 5%) *For more details, please download the PDF or feel free to contact us.

  • FL72565_Core-Flex-shadow1.png
  • Other measurement, recording and measuring instruments

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FlexFire SEC Series Columns for HPLC/UHPLC

FlexFire SEC Series Columns for HPLC/UHPLC

Easily analyze polymer compounds! Methods can also be easily constructed.

The "FlexFire SEC Series" is a column designed for the separation of macromolecules such as proteins and antibodies, as well as low molecular weight compounds, based on their size in solution. This product primarily utilizes isocratic elution, and methods can be easily constructed. Additionally, the results obtained are straightforward, making it suitable for the analysis of intact biomacromolecules such as proteins and antibodies. 【Features】 ■ Easy analysis of macromolecules ■ Separation based on size ■ Primarily uses isocratic elution, with easy method construction ■ Simple results obtained ■ Well-suited for the analysis of intact biomacromolecules such as proteins and antibodies *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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TSKgel UP-SW Series

TSKgel UP-SW Series

Microparticle filler! Rapid analysis of biomacromolecules (15cm) and high-resolution analysis (30cm) are possible.

The "TSKgel UP-SW series" is a size exclusion chromatography that is compatible with both UHPLC and HPLC systems. It is possible to select columns with different molecular weight fractionation ranges according to the molecular weight of the target sample. The surface characteristics of the packing material are the same as the existing "TSKgel SW type." Additionally, we also offer the "TSKgel SuperSW mAb series," "UltraSW Aggregate," and "TSKgel SuperSW, SWXL, SW, BioAssistSWXL series." 【Features】 - Due to the use of fine particle packing materials (2μm/3μm), rapid analysis (15cm) and high-resolution analysis (30cm) of biomacromolecules are possible. - The surface characteristics of the packing material are the same as the existing TSKgel SW type. - Compatible with both UHPLC and HPLC systems. - It is possible to select columns with different molecular weight fractionation ranges according to the molecular weight of the target sample. *For more details, please refer to the PDF document or feel free to contact us.

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HPLC/UHPLC column "FlexFire C30"

HPLC/UHPLC column "FlexFire C30"

Dramatically updated! Not just a second choice, but also active as a main column.

The "FlexFire C30" was introduced in 1998 and has been a representative column used by many people to this day. Updates include an expanded pH range, improved pressure resistance, and enhanced retention and separation of highly polar compounds. Analysis with a 100% aqueous mobile phase can still be set up as before. It serves not just as a second choice, but also as a main column. 【Features】 ■ Expanded pH range ■ Improved pressure resistance ■ Enhanced retention and separation of highly polar compounds ■ Serves as a main column, not just a second choice ■ Utilizes refined silica gel *For more details, please refer to the PDF materials or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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SEC column "TSKgel(R) UP-SW3000-LS"

SEC column "TSKgel(R) UP-SW3000-LS"

Reduced initial protein adsorption! Compatible with both UHPLC and HPLC systems.

The "TSKgel(R) UP-SW3000-LS" is a size exclusion chromatography column for the separation of biomacromolecules, using a filler with a particle size of 2μm that has diol groups chemically bonded to the surface of silica gel. Due to the use of fine particle fillers (2μm), it enables rapid analysis (15cm column) and high-resolution analysis (30cm) of biomacromolecules such as proteins (antibodies, enzymes). It has minimal column shedding, making it suitable for measurements using light scattering detectors. 【Features】 ■ The surface characteristics of the filler are similar to the existing TSKgel SW type. ■ Minimal column shedding makes it ideal for measurements using light scattering detectors. ■ Improved filler and column materials reduce initial protein adsorption. ■ Shipping and storage solvents are easily managed with a 20% ethanol aqueous solution. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.

  • Other physicochemical equipment

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HPLC/UHPLC column "FlexFire AQ C18"

HPLC/UHPLC column "FlexFire AQ C18"

Retention and separation of highly polar compounds in reverse phase systems! It can be handled just like before.

The "FlexFire AQ C18" is a column that can perform well across a wide range in both first and second applications. In reversed-phase columns, retaining and separating highly polar compounds can be extremely challenging. As a result, it is often the case that HILIC columns are chosen, but this column has been developed as an additional option before transitioning to HILIC columns. The frequently used C18 group in familiar reversed-phase columns has no change in usage, allowing for handling in the same manner as before. 【Features】 ■ Suitable for retaining and separating highly polar compounds in reversed-phase systems ■ Can perform well across a wide range in both first and second applications ■ Serves as an additional option before transitioning to HILIC columns ■ Can be handled in the same manner as before ■ Utilizes refined silica gel *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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UHPLC column

UHPLC column

Improve productivity! A lineup of columns that maximizes the performance of UHPLC systems.

Many of the LC columns currently available on the market cannot fully leverage the benefits derived from the performance of the latest UHPLC systems. Waters offers a variety of columns for UHPLC, such as CORTECS columns and eXtended Performance [XP] columns, to assist customers in overcoming challenges in their analyses. This expands the options for column selection and maximizes the potential of analytical laboratories. **Features** - CORTECS 2.7μm columns that utilize solid-core (core-shell) particles to maximize UHPLC separation performance - XP columns that use three types of particle substrates: ethylene-bridged hybrid (BEH), High Strength Silica (HSS), and Charged Surface Hybrid (CSH), providing excellent selectivity and flexibility in method development - Column sizes and particle diameters designed for UHPLC - Seamless method transfer between UPLC, UHPLC, and HPLC - Exceptional reproducibility between batches and columns through comprehensive management of the entire manufacturing process from silica and hybrid synthesis to the final product

  • パーティクル.jpg
  • Separation device
  • Analytical Equipment and Devices

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SEC column "TSKgel UP-SW Aggregate"

SEC column "TSKgel UP-SW Aggregate"

Rapid analysis (15 cm column) and high-resolution analysis (30 cm) of biomacromolecules such as antibody aggregates, high molecular weight proteins, and nucleic acid fragments are possible.

● The surface properties of the packing material are similar to the existing TSKgel SW type. ● Ideal for the separation of high molecular weight aggregates of antibodies, high molecular weight proteins, and nucleic acid fragments. ■ Quick analysis (15 cm column) and high-resolution analysis (30 cm) are possible. ■ The surface properties of the packing material are similar to the existing TSKgel SW type. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.

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SEC column "TSKgel UP-SW2000"

SEC column "TSKgel UP-SW2000"

Due to the microparticle filler (2 μm), rapid analysis of biomacromolecules and mid-sized molecules such as low molecular weight proteins, peptides, and oligonucleotides is possible.

● The surface properties of the packing material are similar to the existing TSKgel SW type. ● It is suitable for the separation of low molecular weight proteins, fragments, peptides, and oligonucleotides. 【Features】 ■ Rapid analysis (15 cm column) and high-resolution analysis (30 cm) are possible. ■ The surface properties of the packing material are similar to the existing TSKgel SW type. ■ Optimized for the separation of antibody (IgG) dimers, monomers, and fragments. ■ Compatible with both UHPLC and HPLC systems. *For more details, please refer to the PDF document or feel free to contact us.*

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Technical Data: The purification of bispecific antibodies is settled with this! (2)

Technical Data: The purification of bispecific antibodies is settled with this! (2)

Selection of chromatography suitable for the separation of aggregated impurities related to antibodies.

During the production of bispecific antibodies (BsAb), various impurities such as mispaired antibodies, aggregates, incomplete antibodies, and fragment antibodies are often generated. Therefore, in the downstream purification process of BsAb, it is important to effectively separate and remove these impurities derived from various products and process-related impurities using chromatography. This report presents the separation modes of chromatography used in the isolation and purification of BsAb that have been previously reported, along with the corresponding packing materials and separation conditions.

  • Chromatographic resins and packing materials

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Bond Elut LMS

Bond Elut LMS

Bond Elut LMS elutes target compounds without the addition of amines, buffers, or acids.

Bond Elut LMS elutes target compounds without the addition of amines, buffers, or acids. The polymer, which eliminates secondary interactions, means that elution of compounds can be achieved with organic solvents or low ionic strength HPLC mobile phase solvents. This feature ensures compatibility with precision analytical techniques such as LC/MS. Bond Elut LMS is characterized by an optimized particle size and a large surface area that enhances extraction efficiency, without interfering with clear extraction. Bond Elut LMS ensures excellent recovery rates and reproducibility, reducing downtime and ensuring trouble-free operation. *For more details, please refer to the PDF materials or feel free to contact us.*

  • Other consumables

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Column "AdvanceBio Amino Acid Analysis (AAA)"

Column "AdvanceBio Amino Acid Analysis (AAA)"

Designed as an innovative solution for the characterization of biomolecules!

The "AdvanceBio Amino Acid Analysis (AAA)" is an LC column for biopharmaceutical analysis that can separate amino acids from protein hydrolysates and cell culture media with high speed, excellent sensitivity, and reproducibility. It includes proven reagents for amino acid derivatization, a prepared amino acid standard kit, columns based on our innovative Poroshell technology, and support from our experts. When used in conjunction with Agilent InfinityLab LC series instruments, it provides a complete solution for amino acid analysis. 【Features】 ■ Achieves high separation capability with the efficient particle morphology of Poroshell ■ Long column life due to robust chemically modified silica with excellent resistance to high pH ■ Utilizes 2.7μm diameter particles, compatible with both HPLC and UHPLC systems ■ Standard solutions and reagents available as a kit ■ Uses a general analysis injection system *For more details, please refer to the PDF document or feel free to contact us.

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SEC column "TSKgel UP-SW3000"

SEC column "TSKgel UP-SW3000"

Due to the microparticle filler, rapid analysis of biomacromolecules such as proteins, antibodies, and enzymes is possible.

The latest column is the UP-SW3000-LS. Please refer to that as well. The "TSKgel UP-SW3000" is a size exclusion chromatography column for the separation of biomacromolecules, using a packing material that chemically bonds diol groups to the surface of silica gel particles with a diameter of 2μm. Due to the fine particle packing material, rapid analysis (15cm column) and high-resolution analysis (30cm) of biomacromolecules such as proteins, antibodies, and enzymes are possible. 【Features】 ■ Enables rapid analysis (15cm column) and high-resolution analysis (30cm) ■ Surface characteristics of the packing material are similar to existing TSKgel SW types ■ Optimized for the separation of antibody (IgG) dimers, monomers, and fragments ■ Compatible with both UHPLC and HPLC systems *For more details, please refer to the PDF document or feel free to contact us.

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