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column(hplc) - List of Manufacturers, Suppliers, Companies and Products

Last Updated: Aggregation Period:Dec 17, 2025~Jan 13, 2026
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column Product List

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Biomonolith <Protein A, Protein G>

Used primarily for the quantification of IgG (calculation of fermentation potency)!

We would like to introduce our bio-pharmaceutical analysis LC column, 'Bio Monolith <Protein A, Protein G>'. It enables high-speed and accurate quantification of mAb antibody titers and allows for the purification of small amounts of mAb for further characterization. It can be used with HPLC and UHPLC systems, including Agilent 1100, 1200, and 1260 Bio Inert Quaternary LC. The Bio Monolith Protein G column is suitable for the purification of IgG molecules that do not bind to Bio Monolith Protein A. 【Features】 ■ Separation analysis of all IgG subclasses (human and mouse) ■ Protein G provides different selectivity for IgG molecules that do not bind to Protein A ■ Separation unaffected by flow rate ■ High-speed movement between mobile and stationary phases ■ Fixation of method parameters ■ Significant reduction in analysis time and buffer usage *For more details, please refer to the related links or feel free to contact us.

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HAS/IgG Multiple Affinity Removal Column

Removal of albumin and IgG! This improves the sensitivity of subsequent sample LC/MS analysis and electrophoresis.

We would like to introduce the "HAS/IgG Multiple Affinity Removal Column" that we handle. The column format is "4.6 x 50mm," and custom-made column sizes and spin cartridges are also available. By using the multiple affinity removal system, approximately 69% of total proteins (albumin and IgG) can be removed. With an optimized two-buffer system, the column can be reused with very high reproducibility for over 200 injections, and it is provided with a convenient column reagent starter kit. 【Features】 ■ The serum injection volume per analysis is 100μL for a column with an inner diameter of 4.6mm. ■ The protocol and column are optimized for the analysis of human biological samples. ■ Compatible with most FPLC or HPLC pumps and equipment, including Agilent 1200 instruments. ■ Human serum albumin/IgG affinity removal spin cartridges are also available. ■ Up to 20,000μL of plasma/serum can be processed until the column's lifespan is reached (for a column with an inner diameter of 4.6mm). *For more details, please refer to the related links or feel free to contact us.

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JP18 Pharmaceutical Test Item LC Analysis Relevant Column

Introduction of each pharmaceutical item! In addition to the specified columns, test items and others are also included.

On our website, we introduce HPLC columns that comply with the 18th edition of the Japanese Pharmacopoeia (Notification No. 220 by the Ministry of Health, Labour and Welfare, dated June 7, 2021). We have listed pharmaceuticals such as "Actinomycin D," "Aclarubicin," "Acrinol Hydrate," and "Azathioprine" by each item. Additionally, if there are no catalog products for the corresponding structure of fillers or column sizes, we indicate "No applicable products." There is a possibility that we can propose custom columns, so please contact us if you have any requests. [Items Listed (Partial)] ■ Actinomycin D ■ Aclarubicin ■ Acrinol Hydrate ■ Azathioprine ■ Acyclovir *For more details, please refer to the related links or feel free to contact us.

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Agilent BioHPLC Column

If all columns and consumables are used correctly, the lab work will proceed smoothly!

The "Agilent BioHPLC Column" is designed to perform high-precision and high-speed characterization analysis of monoclonal antibodies and other intact proteins, aggregates by SEC, charge variants by IEX, intact mass by reversed phase, primary structure, post-translational modifications (PTM), and cleaved glycan analysis by hydrophilic interaction liquid chromatography. To further enhance the productivity of chromatography labs, smooth operations can be achieved if all columns and consumables are used correctly. 【Features】 ■ Achieves fast and consistent biopharmaceutical analysis ■ Designed to provide consistent performance ■ Underlying technology of the column improves accuracy and productivity ■ Supports speeding up analysis and enhancing lab productivity *For more details, please refer to the PDF materials or feel free to contact us.

  • Other laboratory equipment and containers

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BioResolve RP column for protein separation

New column for reverse phase separation of intact proteins and their subunits.

The analysis of proteins with high molecular weight and complex structures is accompanied by many difficulties. Waters has developed a new reversed-phase column based on the feedback and requests from researchers and analysts who are actually working on protein analysis. The BioResolve RP mAb Polyphenyl column uses solid core particles with a particle size of 2.7 μm and incorporates novel functional groups, achieving not only improved separation but also significant improvements in carryover compared to low molecular weight compounds. At the same time, it reduces issues commonly associated with high temperatures and ion-pair reagents used in reversed-phase separation of proteins. Additionally, we offer guard columns and standards specifically designed for protein analysis, along with comprehensive support such as user-friendly procedures and analysis examples for those conducting protein analysis for the first time.

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FlexFire Metal-Free Column

Introduction to LC/MS is also easy! There are analyses that can be made possible with hardware changes!

The "FlexFire Metal-Free Column" is an HPLC/UHPLC column that has a significant effect on compounds such as phosphate groups and metal coordination. Traditionally, these compounds have been analyzed using stainless steel columns with phosphate buffer methods. By using this product, it is possible to develop methods using formate buffers, making it easier to introduce into LC/MS. 【Features】 - Prevents adsorption by avoiding contact with metals - Allows for direct elution of compounds - Has a significant effect on compounds such as phosphate groups and metal coordination - Enables method development using formate buffers *For more details, please refer to the PDF document or feel free to contact us.

  • Other protein analysis
  • Column Chromatography

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Liquid chromatography column "stainless steel empty column"

Accumulated know-how and diverse customer needs are being fulfilled!

Hakusho Co., Ltd. is committed to providing better products based on many years of experience and achievements, keeping in mind the diverse needs of our customers. We offer not only analytical columns but also preparative columns, packing devices, and various connecting parts (adapters). We accept custom orders based on application and dimensions, so we look forward to hearing from you. 【Features】 - Capable of supporting a wide range of HPLC applications - A rich variety of inner diameters, high inner surface precision, and low diffusion column ends - Proposing high-precision products based on years of experience and achievements - Custom empty columns can be manufactured to meet customer needs - Available in screw type and ferrule type (swagelok type) For more details, please contact us or download the catalog.

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DNAPac PA200/PA200 RS Column

High separation of oligonucleotide chains and stereoisomers! Anion exchange column.

The "DNAPac PA200 HPLC/DNAPac PA200 RS (High-Speed Separation) UHPLC Column" is a column designed for the high separation of ssDNA and RNA. The selectivity for oligonucleotides can be controlled by various parameters such as the pH of the mobile phase, solvents, and the use of different salts, allowing for adjustments in separation to meet the specific requirements of applications. We provide structural analysis and quantification methods utilizing high-resolution mass spectrometry and anion exchange columns for UHPLC. 【Features】 ■ High separation of oligonucleotide chains and stereoisomers ■ Improved separation and throughput ■ Control of selectivity and retention through the pH of the mobile phase, type of salt, and type of mobile phase ■ Separation of n from n-1 bases *For more details, please refer to the PDF document or feel free to contact us.

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Column "AdvanceBio MS Spent Media"

Rapid MS-based workflow! No need for sample derivatization, saving time and resources.

The "AdvanceBio MS Spent Media" is a HILIC column that can separate non-derivatized amino acids and other polar metabolites in cell culture media with high speed, excellent sensitivity, and reproducibility, making it suitable for mass spectrometry. By combining it with Agilent InfinityLab LC series instruments and Agilent MS instruments, a comprehensive solution for cell culture component analysis is achieved. The AdvanceBio MS Spent Media analysis is an innovative solution designed for the generation and characterization of biomolecules, as a new product in the Agilent AdvanceBio family. 【Features】 ■ Achieves excellent peak shape and recovery rates even for challenging ionic metabolites ■ Baseline chromatographic separation capability for isomers of leucine and isoleucine ■ Columns tested with amino acids to ensure quality and performance ■ Excellent analytical sensitivity with a mobile phase suitable for MS ■ Compatible with both HPLC and UHPLC systems *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Bonus-RP

Introducing a new technology that enables three-dimensional protection! ZORBAX reverse phase HPLC columns.

The "ZORBAX Bonus-RP" has a unique structure that incorporates polar amide groups into a long alkyl chain. This new bonding method suppresses the interaction between basic compounds and the silica substrate, significantly improving peak shape even for particularly difficult-to-separate basic compounds. It also demonstrates excellent stability at pH 2, making it suitable for improving the peak shape of basic compounds with significant tailing in many other alkyl-type stationary phases. 【Features】 ■ Excellent peak shape for challenging basic compounds at low and neutral pH ■ Unique selectivity among reverse phase columns ■ New technology that embeds polar groups and allows for steric protection ■ Usable with 100% aqueous mobile phases *For more details, please refer to the PDF document or feel free to contact us.

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Technical Data: Chiral Column for Reverse Phase Ultron ES-OVM

Effective for metabolic studies in blood! A column that is valuable in the fields of pharmaceuticals, pesticides, food, and organic synthesis.

The "Ultron ES-OVM" is an optical separation HPLC column that has many excellent features, such as being easy to use in reverse phase mode, having good peak shape, and being suitable for the study of trace metabolites. It uses amino-modified silica gel with proteins that are resistant to denaturation as the stationary phase, allowing for the analysis of plasma samples without any pre-treatment. This document includes the basic properties of the ligand, examples of separation degree adjustment, and case studies of compound analysis using the Ultron ES-OVM. We encourage you to read it. [Contents] ■ Basic properties of the ligand ■ Mechanism of separation ■ Selection of mobile phase ■ Relationship between pH and capacity factor ■ Relationship between salt concentration and capacity factor *For more details, please refer to the PDF document or feel free to contact us.

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ZORBAX Extend-C18

Improvement of retention, separation ability, and peak shape in base compounds! Introducing ZORBAX reverse phase HPLC columns.

The "ZORBAX Extend-C18" utilizes a groundbreaking bidentate C18-C18 bonding technology, enabling the development of high-separation methods in high pH regions while being a silica-based column. The unique bidentate bonding and double-end capping enhance stability at high pH. It improves retention, separation capability, and peak shape for basic compounds. To extend column life, it is preferable to use triethylamine, ammonium hydroxide, or pyrrolidine as buffer solutions, while phosphate buffers are not recommended. 【Features】 - High separation efficiency and long lifespan in high pH regions (up to pH 11.5) - Improved stability at high pH due to unique bidentate bonding and double-end capping - Higher efficiency and superior peak shape compared to polymer-based columns - High sensitivity and separation for LC/MS analysis of peptides and proteins *For more details, please refer to the PDF document or feel free to contact us.

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Bond Elut Atrazine

Bond Elut Atrazine is designed for the extraction of dealkylated metabolites, specifically desethylatrazine and deisopropylatrazine.

Bond Elut Atrazine is designed specifically for the extraction of atrazine from aqueous matrices, as well as its dealkylated metabolites, desethylatrazine and deisopropylatrazine. The 120μm particle size and suitable tube diameter allow for fast flow rates, making it ideal for processing large volumes of water samples. Additionally, the bed mass capacity of Bond Elut Atrazine is optimized to ensure the reliable recovery of atrazine and its polar metabolites, demonstrating sufficient capacity. By using an analytical method that combines Bond Elut Atrazine with Varian's HPLC diode array detector system, quantitative analysis of atrazine and its dealkylated metabolites in river water is possible even at extremely low concentrations (0.02 ppb). This concentration level is significantly lower than the EPA's standard (3 ppb) and the values set by European agencies (0.1 ppb and 0.5 ppb). The rapid and highly reproducible extraction and analysis methods are reliable and can minimize the consumption of organic solvents. *For more details, please refer to the PDF materials or feel free to contact us.*

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Ultra-low pressure particle size 3μm column Sumipax ODS-D-210SLP

Ultra-low pressure HPLC column achieving the highest standard of low pressure with a particle size of 3 μm.

This is a general-purpose column made from high-purity silica gel manufactured based on the technology our company has cultivated over many years. The SUMIPAX ODS series offers a wide range of high-performance columns, each with different characteristics. We believe that by selecting the optimal column from a variety of options with differing specificities, we can meet all analytical needs.

  • Analytical Equipment and Devices

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InfinityLab Poroshell 120 Column

Method development is quick and easy! Compatible with almost all LC systems, with 12 types of fillers available.

The "InfinityLab Poroshell 120 Column" enhances analytical efficiency and reliability, significantly improving the performance of various instruments. Whether you desire superior HPLC performance, want to achieve UHPLC performance at lower pressures, or are seeking the highest level of UHPLC performance, this column is suitable for you. Available in three particle sizes: 1.9μm, 2.7μm, and 4μm, allowing you to choose the appropriate particle size based on your separation needs. 【Features】 ■ A column with innovative chiral stationary phase on porous surface particles ■ High-speed, high-separation column that increases throughput and separation capability ■ Excellent inter-batch reproducibility that consistently delivers reliable separation results ■ Selectable particle sizes Particle sizes of 1.9μm, 2.7μm, and 4μm maximize the performance of methods and instruments *For more details, please refer to the PDF document or feel free to contact us.

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