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Tangential flow filtration (TFF) is a filtration method that applies a constant pressure to the membrane side by flowing liquid samples parallel to the membrane surface, allowing solutes and small molecules to pass through the membrane. Compared to conventional direct flow filtration, TFF is less prone to filter clogging because it is continuously perfused parallel to the membrane surface, enabling efficient and large-capacity ultrafiltration. The Minimate EVO TFF system is suitable for large-scale protein and nucleic acid purification and is equipped with Omega membranes that have a wide range of molecular weight cut-offs (MWCO) from 1K to 1000K. It is also suitable for applications that require the purification of exosomes, which are extracellular nano-vesicles, from large volumes of cell culture media. It has been reported that TFF methods have a higher recovery rate of exosomes from cell culture media compared to ultracentrifugation [1], and there are also proven results with Minimate [2]. If you are considering large-volume sample ultrafiltration, please feel free to contact us. [1] Busatto S., et al., Cells, 7, 273-283 [2] Sung SE., et al., Int J Mol Sci, 22, 2889-2900

The "LV Centramate" is a product designed for the scale-up and scale-down of tangential flow filtration process development, small batch production of biopharmaceutical processes, scale-down testing, and the production of materials for safety and efficiency studies (Phase I clinical trials). With a low residual volume, it achieves high concentration factors from a small starting volume. Since it has the same flow path length as the Centramate cassette holder and Centrasette cassette holder, linear scale-up is possible. In a 2010 study, LV Centramate was used for the concentration of AAV viruses purified from cell culture supernatant [1]. 【Features】 ■ Linear scale-up is possible ■ Can also be used in conjunction with other lab TFF systems ■ Achieves high concentration factors from a small starting volume due to low residual volume ■ Easy connection with SUS316L polished luer lock ports, ensuring the same compatibility characteristics as the production scale Centrasette holder [1] Vandenberghe, et al., Human Gene Therapy. 21. 1251-1257 (2010).

The "Centramate Tangential Flow System for Laboratory Use" is suitable for processing capacities ranging from 1 to 125 liters in process development and small-scale production. Membrane cassette removal is easy without disassembling the system piping. By simply adding membrane cassettes [each cassette has a filtration area of 0.1 m²], the filtration area can be easily expanded to 0.5 m². Additionally, due to the same flow path length, linear scale-up to larger process systems is possible. There is a documented case of desalting antisense oligonucleotides obtained from solid-phase synthesis using the Centramate 1K membrane for nucleic acid synthesis applications. 【Features】 - Easily expand the filtration area to 0.5 m² by simply adding membrane cassettes - Linear scale-up to larger process systems is possible - Easy removal of membrane cassettes - Provided with SUS316L hardware - Various membranes are available for a wide range of applications Boos, J.A. et al., Nucleic Acids Research. 41 e145 (2013)