Cytiva List of Products and Services

Tangential flow filtration (TFF) is a filtration method that applies a constant pressure to the membrane side by flowing liquid samples parallel to the membrane surface, allowing solutes and small molecules to pass through the membrane. Compared to conventional direct flow filtration, TFF is less prone to filter clogging because it is continuously perfused parallel to the membrane surface, enabling efficient and large-capacity ultrafiltration. The Minimate EVO TFF system is suitable for large-scale protein and nucleic acid purification and is equipped with Omega membranes that have a wide range of molecular weight cut-offs (MWCO) from 1K to 1000K. It is also suitable for applications that require the purification of exosomes, which are extracellular nano-vesicles, from large volumes of cell culture media. It has been reported that TFF methods have a higher recovery rate of exosomes from cell culture media compared to ultracentrifugation [1], and there are also proven results with Minimate [2]. If you are considering large-volume sample ultrafiltration, please feel free to contact us. [1] Busatto S., et al., Cells, 7, 273-283 [2] Sung SE., et al., Int J Mol Sci, 22, 2889-2900

NanoSep is a centrifugal device for ultrafiltration that incorporates a membrane (Omega membrane) in a 1.5 mL tube size. It is suitable for sample volumes of 50 - 500 µL and is effective for the purification and desalting of small-scale proteins and nucleic acids. For example, it can be used for sample preparation and SDS removal in protein or peptide analysis using LC/MS. Additionally, recent studies have utilized NanoSep with a 300K MWCO (molecular weight cut-off) for the purification of SARS-CoV-2 virus present in human nasopharyngeal swabs. - Rapid sample processing time - Low protein adsorption Omega (modified PES) membrane - Wide range of molecular weight cut-offs - Leak prevention of concentrated solutions into the receiver using ultrasonic sealing technology

The "Microsep Advance Centrifugal Filtration Filter" is a 15 mL centrifuge tube type filter that can rapidly and accurately concentrate and recover samples up to 5.0 mL. It boasts a high recovery rate of over 90%. The large effective filtration area (3.3 cm²) allows for quick sample processing. The dead-stop structure ensures the retention of the concentrated liquid, and the color coding allows for easy identification. 【Features】 ■ Low protein and DNA adsorption ultrafiltration membrane (Omega membrane) and housing ■ High recovery rate of over 90% ■ Quick sample processing due to the large effective filtration area (3.3 cm²) ■ Dead-stop structure ensures retention of concentrated liquid ■ Rich variety of fractionation molecular weight sizes ■ Easy identification with color coding *For more details, please refer to the PDF materials or feel free to contact us.

The "MacroSep Advance Centrifugal Filtration Filter" is a 50 mL centrifuge tube type filter that quickly concentrates and recovers samples of up to 20 mL while minimizing loss. It features a low protein and DNA adsorption ultrafiltration membrane (Omega membrane) and housing. With a recovery rate of over 90%, it ensures the retention of concentrated liquid due to its dead-stop structure. It offers a wide range of fraction molecular weight sizes and can be easily identified by color coding. Recently, MacroSep 30K has been utilized in the first purification step of research on fluorescent gold nanoclusters using exosomes as templates [1]. **Features** - Low protein and DNA adsorption ultrafiltration membrane (Omega membrane) and housing - High recovery rate of over 90% - Rapid concentration of 20 mL samples to 0.5 mL - Retention of concentrated liquid due to dead-stop structure - Wide range of fraction molecular weight sizes - Easy identification through color coding *For more details, please refer to the PDF document or feel free to contact us.* [1] Lee, S. T. et al., Molecular Science 22. 4433-4441. (2021)

The "Jumbosep Centrifugal Filtration Device" features a unique sealing mechanism that eliminates the need for O-rings, preventing leaks of concentrated liquid into the receiver. It is equipped with a dead stop mechanism to prevent drying and sticking upon completion of centrifugal filtration. The reservoir and receiver can be reused after autoclaving. 【Features】 ■ Rapid sample processing time ■ Over 90% recovery rate using low protein-binding Omega membranes ■ Color-coded for easy identification of a wide range of fraction molecular weights/pore sizes ■ Unique sealing mechanism design eliminates the need for O-rings, preventing leaks of concentrated liquid into the receiver ■ Built-in dead stop mechanism to prevent drying and sticking upon completion of centrifugal filtration ■ Reservoir and receiver can be reused after autoclaving *For more details, please refer to the PDF document or feel free to contact us.

The "LV Centramate" is a product designed for the scale-up and scale-down of tangential flow filtration process development, small batch production of biopharmaceutical processes, scale-down testing, and the production of materials for safety and efficiency studies (Phase I clinical trials). With a low residual volume, it achieves high concentration factors from a small starting volume. Since it has the same flow path length as the Centramate cassette holder and Centrasette cassette holder, linear scale-up is possible. In a 2010 study, LV Centramate was used for the concentration of AAV viruses purified from cell culture supernatant [1]. 【Features】 ■ Linear scale-up is possible ■ Can also be used in conjunction with other lab TFF systems ■ Achieves high concentration factors from a small starting volume due to low residual volume ■ Easy connection with SUS316L polished luer lock ports, ensuring the same compatibility characteristics as the production scale Centrasette holder [1] Vandenberghe, et al., Human Gene Therapy. 21. 1251-1257 (2010).

The "Centramate Tangential Flow System for Laboratory Use" is suitable for processing capacities ranging from 1 to 125 liters in process development and small-scale production. Membrane cassette removal is easy without disassembling the system piping. By simply adding membrane cassettes [each cassette has a filtration area of 0.1 m²], the filtration area can be easily expanded to 0.5 m². Additionally, due to the same flow path length, linear scale-up to larger process systems is possible. There is a documented case of desalting antisense oligonucleotides obtained from solid-phase synthesis using the Centramate 1K membrane for nucleic acid synthesis applications. 【Features】 - Easily expand the filtration area to 0.5 m² by simply adding membrane cassettes - Linear scale-up to larger process systems is possible - Easy removal of membrane cassettes - Provided with SUS316L hardware - Various membranes are available for a wide range of applications Boos, J.A. et al., Nucleic Acids Research. 41 e145 (2013)

The NAB NanoSep Spin Column is equipped with a two-layer silica-based quartz glass fiber membrane, enabling efficient binding of DNA and RNA, as well as rapid processing of samples. The two-layer membrane structure allows for a high maximum nucleic acid binding capacity, accommodating high concentrations of nucleic acid purification depending on the samples processed. The NAB NanoSep can be used as a column for nucleic acid purification, allowing for the extraction of plasmid DNA, genomic DNA, and total RNA from various cell and tissue samples. We can provide a high-quality, high-yield nucleic acid purification system compatible with the nucleic acid purification buffers you are currently using. 【Features】 • Rapid sample processing • Two-layer silica-based quartz glass fiber membrane enabling efficient binding of DNA and RNA • Low-adsorption polypropylene housing • Ultrasonic welding seal that does not cause bypass or sealing defects • Compatible with standard centrifuge rotors 【Material Composition】 Membrane: Two-layer binder-free, silica-based quartz glass fiber Housing: Polypropylene 【Effective Filtration Area】0.28 cm² 【Maximum Sample Capacity】500 μL

The new business fields related to the manufacturing of biopharmaceuticals, such as gene therapy drugs, peptide medicines, and new types of vaccines represented by mRNA vaccines, are on a continuous path of expansion. However, the technology for scaling up from lab-scale to commercial production scale remains a challenge. The equipment and operating methods used in small-scale lab production differ from those used in commercial production scale. Therefore, while leveraging the results of laboratory-level research, it is essential to collect test data that anticipates the transition to GMP manufacturing from the preclinical and clinical trial drug production stages. This will be the first step toward practical application in the future. This seminar will provide an overview of the fundamentals of TFF technology, which is frequently used in manufacturing processes. Date: November 25, 2021 (Thursday) 13:30 ~ 14:30 *An on-demand version is currently available. URL: https://biotech.pall.jp/jp/ja/landing/tff-basics.html?utm_source=e_signature&utm_medium=email&utm_campaign=21-11-121-TFFWB-JP&utm_content=text_link_lab

MabSelect VH3 affinity resin uses a protein A ligand designed to interact exclusively with the variable heavy chain of the human antibody VH3 sequence family. This allows for the efficient separation of fragments such as bispecific antibodies (bsAb), fragment antigen-binding regions (Fab), single-chain variable fragments (scFv), and variable heavy chain domains (VHH). • Interacts only with the variable heavy chain of VH3, not with the Fc region • High binding capacity for antibody fragments with VH3 • Removal of antibody-derived impurities from asymmetric bsAb with VH3 • Stable even after washing with 0.5 M NaOH

Cytiva Protein Select resin allows for the spontaneous cleavage between the tag and the target protein during the affinity step, resulting in the elution of high-purity target protein without any residual amino acids from the tag. 【Self-Cleavage of the Tag】 The Cytiva Protein Select tag binds to the ligand on the resin, and after a certain period, the tag is cleaved from the target protein. This means that no enzymatic digestion is required! 【No Residual Amino Acids from the Tag】 Since the cleavage occurs between the tag and the target, no amino acids from the tag remain in the target protein. 【High Purity in One Step】 The cleaved target can be eluted from the column simply by flowing the starting buffer. There is no need for pH changes or the addition of reagents for elution.