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"Cellufine" is a resin to chromatography used for the purification of antibody drugs such as monoclonal antibodies, biopharmaceuticals like proteins and enzymes, vaccines, virus particles, and food additives. Due to its spherical cellulose particles, it has mechanical strength and high biocompatibility, making it widely used for the purification of biopharmaceuticals. With over 40 years of manufacturing experience, it has been adopted by global pharmaceutical companies around the world. Additionally, as it is derived from natural polysaccharides, it has a lower level of leachables compared to synthetic polymers. 【Features】 - Adopted by pharmaceutical companies worldwide for the purification of biopharmaceuticals - Can be operated at high flow rates - High adsorption performance allows for cost improvements in the manufacturing process - Lower leachables compared to synthetic polymers due to being made from natural polysaccharides - Manufactured under an ISO9001 quality system *For more details, please refer to the PDF document or feel free to contact us.

Cellufine SPA-HC is a highly reusable resin that immobilizes alkaline-resistant Protein A on porous cellulose particles, significantly improving pressure resistance through a unique crosslinking method. With proprietary particle manufacturing technology developed over 40 years of industry experience, it achieves high adsorption performance at high flow rates. Designed as a filler for actual manufacturing scales, it allows for the utilization of existing equipment while achieving reduced lead times. 【Features】 ■ Excellent dynamic binding capacity Achieves excellent dynamic adsorption even with short residence times Over 50 mg/mL excellent adsorption performance at a residence time of 2 minutes ■ High pressure resistance and liquid permeability Achieves high flow rates at low pressure through unique crosslinking technology At an operating pressure of 0.3 MPa, liquid flow is possible at a flow rate with a residence time of 2.4 minutes ■ Robust performance independent of antibody type Maintains high adsorption performance even with different monoclonal antibodies Enables robust downstream development ■ Excellent reusability Immobilizes highly alkaline-resistant recombinant Protein A Achieves excellent alkaline resistance *For more details, please refer to the PDF document or feel free to contact us.

Cellufine Sulfate is an affinity chromatography resin used for virus purification. It has a proven track record of use in vaccine manufacturing by global pharmaceutical companies worldwide. The density gradient ultracentrifugation method used for virus purification has several drawbacks, including lead time, reproducibility, and safety issues. Cellufine Sulfate can address these shortcomings due to its chromatography capabilities. 【Features】 ■ Affinity activity for live viruses, inactivated viruses, and split viruses ■ Affinity for virus-derived antigens, bacteria-derived antigens, and heparin-binding proteins ■ The chromatography process is closed, ensuring high safety and low risk of microbial contamination ■ Effective in removing endotoxins that may have contaminated the manufacturing process, as it does not adsorb endotoxins ■ Due to its negatively charged ligands, it does not adsorb positively charged impurities such as host-derived DNA ■ Physically robust and highly durable as it is based on cellulose ■ Sterilization through multiple autoclave cycles is possible *For more details, please refer to the PDF materials or feel free to contact us.

Cellufine ET Clean is a chromatography resin that immobilizes poly-ε-lysine on spherical cellulose particles. It can efficiently remove endotoxins from the target sample solution. Cellufine ET Clean can effectively remove endotoxins produced by gram-negative bacteria under conditions such as physiological saline. Since endotoxins do not elute even under high salt concentration conditions, it can be suitably used when endotoxins need to be removed from proteins. The product is available in two types: Cellufine ET Clean S: Small pores, resulting in less non-specific adsorption of proteins. Cellufine ET Clean L: Large pores, capable of adsorbing a large amount of endotoxins. 【Features】 - Adsorbs endotoxins at high salt concentrations. - At high salt concentrations where endotoxins are adsorbed, proteins pass through. - Efficiently removes endotoxins from proteins and other substances. - Stable polyamino acids do not become inactive, allowing the use of harsh cleaning solutions. *For more details, please refer to the PDF document or feel free to contact us.

"Cellufine MAX IB" is a new mixed-mode resin used for the polishing purification of monoclonal antibodies (Mab) after the purification of Protein A resin. It features surface modification with polyallylamine and the modification of some parts with butyl groups, allowing proteins to adsorb even at high salt concentrations. 【Features】 ■ Can be used for the polishing purification of antibody drugs ■ After Protein A purification, it can achieve high purity with just one purification step ■ Contributes to reduced lead times and cost savings through a two-step purification process ■ Stable resin that allows for in-place cleaning with 0.5M sodium hydroxide ■ Unique salt tolerance that retains proteins even at high salt concentrations *For more details, please refer to the PDF document or feel free to contact us.

Cellufine MAX GS is a second-generation chromatography resin that allows for use at higher flow rates. By utilizing surface modification technology, it can be used at high flow rates while also possessing high dynamic binding capacity. It can be used as a high-performance chromatography resin, which is expected to enhance downstream processing capacity. Cellufine MAX GS is a new strong cation exchange chromatography resin optimized for ligand concentration. It demonstrates excellent performance in removing aggregates from monoclonal antibodies. 【Features】 - Can be used for polishing purification of antibody drugs - Capable of separating antibody dimers and aggregates - Unique salt tolerance that allows protein adsorption even at high salt concentrations - Excellent flow rate characteristics due to advanced crosslinking technology - Contributes to the reduction of the amount of support used due to high adsorption capacity *For more details, please refer to the PDF document or feel free to contact us.

Due to advances in vaccines and clinical diagnostics, there is a growing demand for highly purified and concentrated large amounts of viral particles. "Cellufine Sulfate" is a chromatography carrier. This allows for the avoidance of cumbersome, time-consuming, and potentially dangerous conventional ultracentrifugation methods. In this document, we present an example of the purification of human coronavirus OC43 (hCoVOC43) using our product, which has extensive experience in virus purification. We encourage you to read it. [Contents (partial)] ■ Purification of hCoV OC43 viral particles ■ Evaluation of the virus by hemagglutination titer (HA titer) ■ Evaluation of host-derived DNA and protein amounts ■ Summary *For more details, please refer to the PDF document or feel free to contact us.

"Cellufine" is a chromatography resin that has various ligands (functional groups) modified on the surface of spherical porous cellulose particles. It is used in the manufacturing of biopharmaceuticals such as antibody drugs, vaccines, and protein formulations. This document reports a method to achieve high-purity purification of monoclonal antibodies through a two-step purification process using the Protein A resin Cellufine SPA-HC for the capture step and Cellufine MAX IB for the polishing step. Since it allows for high-purity purification in two steps, it can streamline the process and reduce lead times. We encourage you to read it. [Contents (partial)] ■ Overview of purification ■ Protein A chromatography ■ Mixed-mode chromatography ■ Summary *For more details, please refer to the PDF document or feel free to contact us.

In the downstream (purification process) of monoclonal antibody (mAb) manufacturing, it is common to use chromatography resin for the Protein A column capture step and the polishing step, which is called impurity removal. This document introduces a method that connects two columns used in the polishing step and employs them in flow-through mode. By using this method, we were able to efficiently remove impurities without the cumbersome operations of adjusting pH and electrical conductivity between the polishing process columns. [Contents] ■ Introduction ■ Experimental Methods ■ Analysis ■ Results ■ Conclusion ■ Acknowledgments *For more details, please refer to the PDF document or feel free to contact us.

Cellufine offers a range of ion exchange chromatography resins. Each resin has its own characteristics, and it is important to select the resin that is suitable for the purification of the target protein. This document introduces the features of each resin sold as Cellufine cation exchange chromatography. We encourage you to read it. *You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.

"Cellufine MAX IB" is a new mixed-mode resin used for the purification of monoclonal antibodies (Mab) after the purification of Protein A resin. By surface modification of polyallylamine and modifying some of it with butyl groups, it has the characteristic of protein adsorption even at high salt concentrations. This document introduces the basic features and chemical stability. Please take a moment to read it. [Contents (partial)] ■ Flow rate characteristics of Cellufine MAX IB ■ Cellufine MAX IB with excellent salt resistance ■ Recommended cleaning solution (CIP) ■ Mab purification after Protein A chromatography ■ Conclusion *For more details, please refer to the PDF document or feel free to contact us.

This document is a technical data sheet published about the ion exchange chromatography resin "Cellufine MAX GS." The base carrier uses highly cross-linked cellulose particles, allowing for operation at high flow rates. The ligand structure is designed to efficiently separate monomers and aggregates of monoclonal antibodies. We introduce topics such as "separation of monomers and aggregates of monoclonal antibodies" and "separation characteristics of model proteins." We encourage you to read it. [Contents (partial)] ■ About Cellufine ■ Cellufine MAX GS ■ Dynamic binding capacity of Cellufine MAX GS ■ Separation of monomers and aggregates of monoclonal antibodies ■ Flow rate characteristics of Cellufine MAX GS *For more details, please refer to the PDF document or feel free to contact us.

"Cellufine ET Clean" is a chromatography resin that immobilizes ε-polylysine on cellulose particles. By strongly adsorbing endotoxins present in the sample solution onto the resins, it enables the removal of endotoxins from the sample. This document provides a detailed explanation of the product features and examples of endotoxin removal using graphs. We encourage you to read it. [Contents (partial)] ■ A new chromatography resin for endotoxin removal ■ Chemical structure ■ Product features ■ Selectivity for endotoxins (LPS) from bovine serum albumin (BSA) ■ Endotoxin removal performance from various proteins *For more details, please refer to the PDF document or feel free to contact us.

"Cellufine Sulfate" is an affinity chromatography resin. It can be used for the concentration and purification of viruses, removal of pyrogens from viruses, purification of virus-like particles (VLPs), and purification of proteins with affinity activity, such as bacterial-derived antigens and serum-derived proteins. This document includes flow packing procedures and examples of packing columns with inner diameters of 3.2 cm, 30 cm, and 45 cm. We encourage you to read it. [Contents (partial)] ■ Flow packing procedures ■ Example of packing a 3.2 cm inner diameter column ■ Example of packing a 30 cm inner diameter column ■ Example of packing a 45 cm inner diameter column ■ Results and discussion *For more details, please refer to the PDF document or feel free to contact us.

"Cellufine Sulfate" is an affinity chromatography resin that has binding activity to viruses and serum proteins. It can be used for the purification of viruses, virus-derived antigen proteins, serum proteins, and heparin-binding proteins. This document presents a case study of the purification of influenza virus derived from chicken egg culture. By passing a near-neutral culture solution through it and increasing the salt concentration, the virus can be eluted, allowing for simple and efficient purification and concentration of the virus. *You can download the English version of the catalog. *For more details, please refer to the PDF document or feel free to contact us.

This document is the technical data sheet for the affinity chromatography resin "Cellufine Sulfate" handled by our company. It can be used for the purification of viruses, virus-derived antigen proteins, serum proteins, and heparin-binding proteins. It provides detailed explanations of purification examples for influenza virus, rabies virus, and endotoxin removal (depyrogenation). We encourage you to read it to assist in the process design of biopharmaceuticals. [Contents (partial)] ■ Features ■ Benefits ■ Examples of virus purification with proven adsorption and virus-derived/bacterial-derived antigens ■ Example of rabies virus purification ■ Example of influenza virus purification *For more details, please refer to the PDF document or feel free to contact us.

"Cellufine" is a chromatography resin that has various ligands (functional groups) modified on the surface of spherical porous cellulose particles. It is used in the production of biopharmaceuticals such as antibody drugs, vaccines, and protein formulations. This document presents an example of high-purity purification from CHO cell culture supernatant using Cellufine in a three-step process. We encourage you to read it. [Contents (partial)] ■ Purification procedure ■ Protein A chromatography process (Cellufine SPA-HC) ■ Virus inactivation by acid treatment ■ Anion exchange chromatography process (Cellufine MAX Q-h) ■ Cation exchange chromatography process (Cellufine MAX GS) *For more details, please refer to the PDF document or feel free to contact us.

"Cellufine SPA-HC" is a chromatography resin in which highly cross-linked spherical cellulose particles are immobilized with a highly alkaline-resistant Protein A ligand. It features excellent flow characteristics, low Protein A elution levels, and high dynamic binding capacity. Additionally, it maintains good adsorption activity even after multiple cycles of cleaning in place (CIP). This document provides a detailed explanation of "performance and features," including comparisons of dynamic binding capacity with various commercially available resin and comparisons of DBC for mAb and pAb, using diagrams and graphs. We encourage you to read it. [Contents (partial)] ■ Excellent dynamic binding capacity (DBC) ■ Comparison of adsorption amounts of monoclonal antibodies ■ Purification of monoclonal antibodies from CHO cell culture samples ■ High-throughput screening of elution pH ■ CIP stability of Cellufine SPA-HC *For more details, please refer to the PDF document or feel free to contact us.

Cellufine Phosphate is an affinity chromatography resin that has an affinity for nucleic acid-binding proteins. T7 RNA polymerase can be efficiently purified using Cellufine Phosphate due to its nature as a nucleic acid-binding protein. T7 RNA polymerase is an RNA synthesis enzyme used to produce mRNA from DNA templates. In the manufacturing process of mRNA vaccines, which became famous as a vaccine for the novel coronavirus disease (COVID-19), T7 RNA polymerase is used as the enzyme for mRNA synthesis. T7 RNA polymerase can be purified to high purity through a two-step purification process using Cellufine Phosphate and Cellufine MAX DEAE (a weak anion exchange support). *For more details, please refer to the PDF document or feel free to contact us.

Cellulose resin MLP1000 DexS is a chromatography resin that is based on the next-generation cellulose resin MLP1000, with sulfated polysaccharides immobilized as ligands. It features highly cross-linked cellulose particles with a large pore diameter and interconnected pore structure. This allows for the use of the internal adsorption sites of the carrier in the purification of large molecular weight virus-like particles, demonstrating extremely high adsorption and purification capabilities. The PDF document presents examples of the purification of inactivated influenza viruses. We provide examples of high-purity purification achieved through chromatography using the cellulose carrier MLP1000 DexS. [Handling of the Development Product] Cellulose resin MLP1000 DexS is a development product. There is a possibility that its quality and name may change. We are providing a limited number of samples of this product as a development item. Companies (in pharmaceutical manufacturing, diagnostic reagents, and other manufacturing, etc.) and researchers who wish to consider this product for manufacturing purposes are requested to agree to this and, taking into account the intended use, we will provide samples. *For more details, please refer to the PDF document or feel free to contact us.

Streptococcus pneumoniae is one of the major pathogens causing high levels of morbidity and mortality from pneumonia worldwide, particularly in children and the elderly. Pneumococcal vaccines based on the capsular polysaccharide (CPS), one of the most important virulence factors, are used to prevent these infections. By using a two-step chromatography purification process for the purification of capsular polysaccharides, it is possible to obtain high-purity vaccines. This process employs hydrophobic interaction chromatography (HIC) and anion exchange chromatography (AEX). We introduce two optimized chromatography media for this process: Cellufine MAX Butyl HS and Cellufine MAX Q-hv. This document provides a detailed explanation of the product features and practical examples related to the chromatography purification of pneumococcal vaccines. We encourage you to read it. *For more details, please refer to the PDF document or feel free to contact us.*