[Next-Generation Sequencing Analysis Case] 12S Amplicon Analysis
We present a case that suggests PCR repetition is effective in increasing the number of fish species detected! *Case study collection available.
We would like to introduce an example analyzed using MiFish, which is used for DNA metabarcoding of fish. Water samples were collected from multiple locations in the Sagami River system and pooled in a plastic tank. To prevent DNA degradation, 1 ml/L of Osban disinfectant was added immediately after sampling. The samples were brought back at room temperature, and filtration and DNA extraction were performed in the lab. The obtained data was provided to MitoFish for homology search. As a result, it was observed that the number of fish species detected increased, suggesting that PCR repetition is effective in increasing the number of detected fish species. [Case Overview] - Filtration and DNA extraction were performed in the lab. - NGS library preparation was conducted using the extracted DNA. - Library preparation was performed in 8 repetitions. - The prepared library was sequenced using Illumina's MiSeq. - The obtained data was provided to MitoFish for homology search. *For more details, please refer to the PDF document or feel free to contact us.*
- Company:ファスマック
- Price:Other